SUMO

PPM1D (1 - 605)

Long Name: 
protein phosphatase, Mg2+/Mn2+ dependent 1D
Accession: 
NM_003620.4
Catalog Number (DU Number): 
DU32201
Protein Activity: 
Expression: 
Purification Method: 
GSH Agarose
Mono-Isotopic Mass: 
94, 417.99 daltons
Average Mass: 
94, 478.37 daltons
Theoretical PI: 
8.67
Purity: 
>80 %
Storage Buffer: 
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 2 mM MnCl2, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature: 
-80 deg C
Native Sequence: 
Amino acids M1 – C605 (end) of human PPM1D. Residue M243 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage: 
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites: 
Not1 sites of pGEX6P-2
Target Family: 
Price: £125.00
Type: 
Species: 
Tag: 
Terminus and Tag: 
N-Term
GST
Uncleaved
Uncleaved
Uncleaved
Uncleaved
Extra Protein: 
No
Clone reference: 
Protein Complete: 
No
Protein Date Complete: 
Wednesday, 4 September, 2019 - 11
Auto Generated: 
Yes
Final Product: 
Received Clone: 
No
LRRK2 ASAP: 
No
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72930
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H 33-end S124E S211E
Expressed: 
6HIS SUMO PPM1H 33-end S124E S211E
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
See snapgene file
Price: £125.00
Completion Time: 
27
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72909
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H 33-end S211E
Expressed: 
6HIS SUMO PPM1H 33-end S211E
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
See snapgene file
Price: £125.00
Completion Time: 
22
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72869
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H 33-end S124E
Expressed: 
6HIS SUMO PPM1H 33-end S124E
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
See snapgene file
Price: £125.00
Completion Time: 
12
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72788
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H 33-end S124A S221A
Expressed: 
6HIS SUMO PPM1H 33-end S124A S221A
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
See snapgene file
Price: £125.00
Completion Time: 
28
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72789
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H S124A S221A
Expressed: 
6HIS SUMO PPM1H S124A S221A
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
See snapgene file
Price: £125.00
Completion Time: 
28
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72776
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H S221A linker deleted
Expressed: 
6HIS SUMO PPM1H S221A
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGAPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*
Price: £125.00
Completion Time: 
10
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72775
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H 33-end S221A linker deleted
Expressed: 
6HIS SUMO PPM1H S221A 33-end
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGAPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*AAA*
Price: £125.00
Completion Time: 
10
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72773
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H S33-end linker deleted S124A
Expressed: 
6HIS SUMO PPM1H S33-end S124A
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSALPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*
Price: £125.00
Completion Time: 
10
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

PPM1H

cDNA Synonyms: 
na
DU Number: 
DU72774
Cost of Production: 
100.00
Plasmid: 
pET15b 6HIS SUMO PPM1H linker deleted S124A
Expressed: 
6HIS SUMO PPM1H S124A
Genbank: 
Q9ULR3
Species: 
Human
Vector Type: 
Bacterial
Tag: 
Amino Acid Sequence: 
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSALPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGNEFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGSDDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*
Price: £125.00
Completion Time: 
10
Target Family: 
Wild-type or Mutant: 
New Protein: 
No
Low Temp: 
No
Hidden Project: 
No
Cost Centre: 
357.811523.R10
Extra Protein: 
No
Cleavage Site: 
Restricted Distribution: 
Not Restricted
COVID-19: 
No
Influenza: 
No
Ubiquitylation Site: 
No
Cost Centre: 
Project Code: 
118922
Project Type: 
D
Budget Group: 
C001
Funder Code: 
2585
Budget Centre: 
1046
Nominal Code: 
35907

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