	   SnapGene      }ctgcagcctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggggcgccagtcctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatttgggggctcgtccgggatcgggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggccagcaacttatctgtgtctgtccgattgtctagtgtctatgactgattttatgcgcctgcgtcggtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagttctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtggcccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggtaggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaaccgaagccgcgcgtcttgtctgctgcagcatcgttctgtgttgtctctgtctgactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagtttgaccttagatcactggaaagatgtcgagcggctcgctcacaaccagtcggtagatgtcaagaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggccgcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacctggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttttgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcttccatccgcgccgtctctcccccttgaacctcctctttcgaccccgcctcaatcctccctttatccagccctcactccttctctaggcgccggccggatctgccaccatggactacaaggacgatgacgataagggatcctctgaattcgaggtcgactcgctcgagagtgcggccgccctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagcttgccagctggggcgccctctggtaaggttgggaagccctgcagatctgatcccggggggcaatgagatatgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaatttagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagagtagatgccgaccgggatctatcgatgcggcgggtgtggtggttacgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggaaacgcctggtatcttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcatcgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggcatggaaaaatacataactgagaatagagaagttcagatcaaggtcaggaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggggcgccagtcctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatttccgacttgtggtctcgctgccttgggagggtctcctctgagtgattgactacccgtcagcgggggtcttcacatgcagcatgtatcaaaattaatttggttttttttcttaagtatttacattaaatggccatagttgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttgcggccgtaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac  6   !               $                  !            )                                                                                    "                #         "   *                      ,   !   %      $   "                               $         "                         6                                                                  	               
                                                      #                                 #                                                   	                                    '            !                  %            1             &                                                      $   '   '   #      1                                          *   %   %                              $      '   !   %      )   .            $         !   $         -        ! K+,-*	> !klQHZ p+	
J3?FRO*  l	Y
\c]"Jm [  < Z  L.`	M


.^aI 		
)
3

&4G$ K N,5 @FO]P]:  d 2Jq		=
K}hS5+FY3  Xa+	g		

h)Y  Z]b(> # n md
	Jr#8rd I
^
{
ed^@C!\!   j		a

A #8Kn2rP M@ 	N


YN5X% J A/	x	,

/ 6  I		
G

AL;)  = [  "!6wFa			
 _r}Apbm e				*
,1H  <N5[6'*1	8+i c;
U1! *78S		
%
4
u

)5ryjf/ T]q	
 3	Q9Vug^mc  <jrA/^;MJp 	 T ;z-6,XK? C L    OFx!GPEb# H    3e(	P	X		



@TLu  e k7Qs~iT6#4 [<Q			yY,:B dKr %f	>

L;GS*XZk >bhQ,		


p s$/		Z _MYh		
im1]    P>ig

F*4%- ! l  
`c?Lp6V<?p &	Wh t2Ew[	
^Yt   R9		 :)7.u 
=o" $ o ms9tfx v| 	
Q

K$s^eC ne
 yb
|
e4\jf k_f8wA|%#Dv"   J`_j" + v +rqo	6zSd {3Aq] ea/XA		

VZv 9]5		Be3_  4@G7^c|kTyb

[$9Lo(   T8F=	t		
(U=sQ#  		'
E

WZi!6&nA1 :O4			
O)K _\w;	O

ktT" NYZA 	D	


+hjYkhb*  ( s  jF	

:



wyZjq  '$=0e	

0{lwKx9 m|)-F	-
\
| BTy		"-d Q g	U	
tBEU>lrc o
/+M  C [	
6
w

w7W^l.[, 7  #,wJ		H
W
*r{
*fm4<	H0! W7CG	Ob			B


	C+qZcn%  > \  P#"P	Hd	`s~xVw   [1B:nF<AAB$ 1 |   9hx.KW`lv}l	
"" ;   oX
-2=IP)`Y  c  `f	<
4%Eg(\XEX ]i	@q ?H			+
 4q|$  6 7t
:(00  B   \

+2
JaUlb"I G    c6?:K9RAj =Oh
o, D8	>KV;os-t <]e$ * u  j*p_nv5yRz2{i"ip Sd8Es	UT v3		&

*sh Sg@0" ' r  &l(<

!v{kvJ  P +nvT	5
v

6Tz)eG   8V-A9
 wUY  h_n3?'3p{W(D_W A  5bgn` ^mr	T4:hndh,s & q U'@
u
Rv? 4&?l22_ozC>+r6 3%=>knyABab;<=*<NKLq  
0\cvMU  _ &s!	}BcP=OQ  2		1d @ 
 U 0|	7-Y;M
NV <{4.
]  

o2NLw{ ,CW>  D M `  '			~"HQdc
R  )rQfGy"	k
&FFz$P   ? P%J?tJQz  I X  4~V)		

M\  Kf3:Q	
26e!(-  				~f 2vA  W"+Y	U


AUw C8tR.cW 1}l	R$UNO   V f 8S
Zj9 	# <7$5 \='C		^
Z7% 5;^  R=/	
zA+`-C;Eh	 |z	 og?


QY   Ve	q
<O&M>xGy4  h .~L/!H	MpMlugH|[ x)s[&	/

3DT+l
 ?	
>J	 ci-		q/ -R`#? 	

iDXj2  E N a  (tV	#IReH.[ =]		
S %	!"ud&7f 0	
P `Nz	#	i		
'  gsZG^GR * 
n2{% G4RgHl
$j.|Q QKLu2	
G`z2&8`   S@h
;KR  !&DQs?j{	%g+9N  @pY5a&.{  Y )_	
]E J MW *			

gN9# " m  	
`
z
adIq7=c1q ! 5v		

@MW@l b4R	q)	Ti7h.  S   :y;EqW.D8tf> P$e
	'	W	
X")  ^Lg#=		f
~
l3\   k  1
v	%	
_~o95g6 u{	


n!wB  x	7c3ue 37?Fb{W\	
ZY<^'  X^:	
Vjm#  i#,ESd	9

zxw8 "Bf	Z	V
sv.Dq*q/ 0 {   Om|k	BG!;@*v >p#X  
9>/TQH Ci7DuS mu2dF % p amSt'g 2~$[`uO)P n1:  q+IPe	%IV	Ey  W JV!9
T
[ R
Q  B@_$:D  g w-}(	p
PLk%Ctf=Fx 	h=I \
 !-O%6	 f
 o$:_y`18~
%_ L(^	
_
y

fDb0k8  ]"	e
}
pC[7g= >az	
}45]& ADc	8
yl l61EG *H` &Z_(  ,U'P?B<E~w# g9^x0}	$^  @K ]	|Ba/k< T)G&0YD}'F' S>?n{.X   Eoa	
`',D]i F}p		"

aTeL|MEr p	7(rK!  
s9{b		Y`nE^4B , w  ,r	9{- ! ^j IbG0		
[M}NF ~YhqN	#


Rws p~	
&WZ fB		@

(bU\f<% 9    :6	r
8CPf'NxH` .f2
l=}0?yU T	)a4~25+ EWq2J^	s,h!L6z1  i  /5y8dtMw"Uc	
q,jmaoRho	  AH<n
N }  _l		c
\N%p5IC\ }:0|zIL	Z:MF_vS)& X94Tis~|>	u	0
J
Y


).">U_   y*	:E>m - x U	6t	4V|OUtJR  %-"G\'K		tH,k Jk*	L


I[b\jN 
G 1I		(
2

|4\2  cH	"7?~'BO 5  @Ew		F


XKo: );MZ5d			$
+0x*e  Siwp	Lo Z;dj	O		



SPrxWt 
Pr~.	
E0$ sQ		XU P

ls qa$[@ ` 6].Sx4	<	@	

'ud Y[9N	C		
D

jgi(a {E		[



,Yipc$ 
)V]k=	-Zkl3b' OgZ0BJV_kuN	A

Ec@'  : b    \WG	;

$<DfO[# F    
s

9JI/@ka! ) t  R)o7	=Jx Qy\zro1  O   %k,;u	

 Sgz(uITX @   g^fm>&V  ^ % 	
1|b1mx@:T  /{31			

M~zL	 >($>		j
y }U*	v}e5'1, B	"#b8 y&	]
*6g  nU.ZG	.
3 _	.i"1 <U
tGe F3	#-Q{~ CXrRK1	z	fM7  u			
H*  R  aO9x#Dd<p		V		k(V-Ss
%  j  06zuV$	

6Xn8b;md%   / z !]ehNl{j	ru-CF~p?)ipv  Bht=SP  p#Od0HD~ IAo
O [
_,HN `!
7
d
x

xo3C6  +]O>/] D(Fm%\	&C\W|& 	8		!
8X_mqKJ LD HM	}	%'[;$ 8   -DS11Ik|+/`gw 5}1T* x~;vK	m
+iQnR' $W3!5Sh}{J	I
X
sNG=
IT! 4  jDH)v	1
K


Ha{39[% (Y	:RL}vc	o	


*Dw/#dVn r5P3	?		
cr) /X8zIU^jtM
M	C
Z

,?'oa`.    Q$[+
n<I;ty?SWn  ? ]    z#			i
a%L  TAYx	
0 ;QeF0}x$  . y   \tC;ko	5GW},7(:u "Nc@CO  'E $7=BBJVK!  CV2R
 0u|	&hPS$ 3 ~ 'iq.yH](L~n	
vbm   :Z	Xmw		$//|   B#/Mb6'I- 2 }   &ALa	m	#u-.l  .
  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                         8                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            )                                                                                     
                          
                                                                                                            
                                     	   	                                       	         -   &                     	   
                                    
   '                                                   )   #          "         )   !      |   ;      #      !         R                      8                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                )                                 
          
                                      
             	 	             	   - &       	 
            
 '                 ) #    "   ) !  | ;  #  !   R   m*  n  n  n  sB  w  },	
mUz	 3 4 ~   BC87Po		
/Bbct,C1]	 ) t =x_s/	;pmZzQt( s $ey	w7( s #G$WFe;	y	
-

kwr!Zks		1]-Nk	k	 
	i
t
y  7  
 ( "c
8UG (Q0

jzm MmA@ x	%
/>! 5E	
ol;z-sM@ Q idg*R	y.R	'Hi(v R	 ! q*7yQ.}	M@ o)v
53m  hm,M@ X  MmNA@q x		G

;\		=okTW 8KN+2 }   iZT	()c		C
*;%B0

z20C8hNE
oE
EoWx<T *7R	 y.4+d T%B#[xC)}F		6

whsVT	C
)u|dJqKFRYj	qi(C
Y'Tis~>*y e i		s= 
erCpz+|
R	|

 YL			0
c



lCM54>+i|	."/eCO 	C
Sgab	c  hO SkoT
f
XN Id Jq=
KFYr%	
S*k [t
;G|y9L(<N+ gj

tBE>y < Z     w`	
^)arP3 ~ ij()K

HaWqJL6   
)rP j

ygtBE> YH  3(	
L?
JO	
q Wgj7		
B
E


WtBE>y+nn3 ~  ij[t
()K

Ha;Gm-F-
 X	

@T		Qn<Iyn2Z.
8   wa(S
 F(#	2	
>[8   a(Sw7	=Jr	

_\w;	t.yH]L~
Zic	i K`.P@	
u X980w"c|r

)j8.R"	U`7JKV^]f8w|#x		pICci ]	KW7		B
E

W+nn	7	=Jr:e(	4>	Y[9N	C		
D

jgi(a/X8zIU^jtM
M	C
Z

,?'oa` 		'
E

WZi!6&nA1ne
P +nvT	5
v

6Tz)eGNYZA 	D	


+hjYkhb Bht=SPdKr %f	>

L;GS*XZk = [  "!6wFa			
 _r}Apbm4  jDH)v	1
K


Ha{39[}U*	v}e5'1,  6 = P [    +8"nvV!6w-FA9I	T	a				
5
G
v




 A6T_z)r}e ALwp;UYbGm W JV}U!*B9		
R
T
"Q#[v}eb5'1,8-R`#? i#,ESd	9

zxw8<{4.
] CV2R
 0u|	&hPSe				*
,1H*H` &Z_(P  +nv!&-DQ]isR?`jT	{		5
v

%6g@T+z)9eq#NG?z~YhqN	#


Rws   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  g<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="HygR" directionality="1" translationMW="38032.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1851-2876" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31543.33" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6071" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5280-6071" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6072-6140" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="9" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3549-4142" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4514-5102" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6141-6245" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1361-1370" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ^<Notes>
<UUID>312c79f4-56e1-4c54-bf01-9aeeeed3a44b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="9:24:15">2020.3.3</Created>
<LastModified UTC="9:24:15">2020.3.3</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         DSTT                                                                                                                  HO  d              E             Y?                                                                                                                                                               <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="0" name="DSTT" enzymeNames="AgeI ApaI AscI BamHI BbsI BglII BsmBI BssHII EcoRI EcoRV HindIII KpnI MfeI NcoI NdeI NheI NotI NsiI PvuI SacI SacII SalI SmaI SpeI SphI XbaI XhoI XmaI"/></CustomEnzymeSets>
