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                                             	               	                                             +         #   $               "               ,      $                     	                                                      !      '            "                  %            3                      !                  
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^
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YG1 	 T ;
d  C L    O$r		yS\#De H    3erk"	f	i		&Xr6  e k!		s*u [<Q*4	
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4I5Nl'n9 v|
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l~%e 
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?'7  W

l8fes u,sg$ 1}l$	tyAT   V f "+fvFH 	 \=lX 
Ih{T  R=1	

zMb!)	 +5cW oVL		ap   VeL.	C9m:  h .~Lskz		
-x6(	=|QZ x)HDS]AKj-	 ? ci(V			g
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&U^q	\ =]wo.	Mf J	_6f%' &b		J `Nt		pO_M  gsZ	v8 *SG
R<a G4	2
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)psjbg  !&DQs'.e)
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iWr J MWt}d			


(Z# " m  @	V	
Q2}P$Qr{U2B$ ! 5vx1	"
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t$8nwZ~"   k  1
vprk			




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@/B8  xB$Z6& 37?Fb{CQMt
a  X^^	}+.,  iH@	m	v	
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
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h1D  h	 Ci
 -tq muv%Tu % p am	5]m(o 2~$%_
uzBjJ!6@O	 xK}kV}U  q+I9N
:  W JVv

gIF #EG  a S  g w-}(,wB5'{@9 	
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Y AG
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 *H`J\i| jU|  ,U' ?8E r
d>`]_!  @K2D
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Xbxcn* F61&=KCt3 pF	3oTd"  
s9,		d!/YXyo , w  ,	-
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8h
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/U^Iml ` 6].f`N_e%! Y[9R'	`	-DM(*S" {{b	:)Ka*1$ =4	,,Mz-o#2 OgZ0B)	~	
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KVi' @   g^}Jk0	<	o	x		P
V
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#nE.9cgz  /{x/
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'!N:K }U
n"k7>&@d t+rf#S 
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/z#.[`{%.   / z !]ehNg	6	]	u				
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y
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 05%
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1F=Kl
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6K#Rt
7 2 }   &A9	



5Jk<AhB6Qqrshij-  0  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        @                                                                                                                                                                                                                                                                                                               &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                                                       	   
       '                           1                                                                                           #                 	                             	                                     
                                                                                      	               &                     	            2   7                     
   	               
                           
            	                                 !   3         "   *         1   "         9      ,      &         _                     @                                                                                                                                             &                                                                                                                                                                                                                                                                                                  	                           	 
   '         1                                 #       	           	             
                                	     &       	    2 7       
 	     
         
    	           ! 3   " *   1 "   9  ,  &   _   om  q  qC  q  vO  {  || 3 4 ~   BC87|		/

h>tCDRS#$5G
 ) t 9_
opm}w8;G(( s n	
87( s #G|*	8	n		L

@+8A!83pT:
+(y	kr	N	kk	@2(

2@+Ry*+(KGK%R,~
5
Vt/,RQX5
VQd:  7  G
iV
 +		z	P{{ +	,F	Te;m} MmA@W52]}ud! 5E7

Xn0-;4SM@^5c9]2	[%g*:_D_D'Hi	7XyI{Ty
 X_D! 7
Xn	*7H:GpAM@5]0
v_yV  h.M@2	5N]  MmNA@~	+W		

5GR2]}Cju	mzTN	2 }   iZT(	y


mCtByTC=_	)E
0

	UY
ET
Tv0e<S	TmQ *7XH:G_D% Ty
mC|#[xCI_	w	{	


J*<)4YTm
6=%Jq	+2Ni	y =R\gmB*: e *u	4mzS 

e	
1
laF	_ND Qn{	TX/NCO <_(yn" $  hO _[hPUXGrD
 
B Jq	
GR\, [t(/|	5



2Vt@|	f<N	S	 gj	5	\	

L
rL:{J < Z     w",2		W@Lj"33 ~ ij[		/Cyz_9c
tWqG'
ke   ,	W3 jL:Jg	5	\	

L
r{ J,	BIhH  3r&X?2 Wgj7	5	\	

L
~

(+r8L:Q{//J3 ~  ij[t(/[|			5



2Vt/Cyz@_m		
n}X rQ`z	Wa:Mx/2CJi8   H8~aq+	 

/(dL]F\_8   aHq8~a{		o3N%	9_\_d.y;1F=K'=If*c*4	s`.fh
	N_ X980O		hDX!7		AX	*d
q
j=_}9		}1
Rc	sd
j}*4W7~

(+8Q//a{		o3N&4kY[9R'	`	-DM(*S"/X8z<
2>GS]h),>Z9L(>JRn ynC0"! ~
f
*w/ne\	E
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gdKrUj
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8

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8



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t m9x`8QE<0
yHLWV CV2R
 05%
$/SfF;	s*H`J\i| jU|P  +nv!&-DQ]is'.L*<emx		)
b
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q `;&2ET@Uxe ;h7M	Pbq4  #               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=Ae5/N
Ѓʒ581:-7+`*S}!sO1 |q*m,W|с_x;+
b8sHӜy@Їʸ0kD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  v    >       -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="AmpR" directionality="2" translationMW="31543.33" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5415" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4624-5415" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5416-5484" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21531.55" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2195-2794" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2893-3486" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3858-4446" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5485-5589" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1930" maxFusedRunOn="1930" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2036-2171" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1856-2185" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="aGFP16" directionality="1" translationMW="14125.82" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1930" maxFusedRunOn="1930" prioritize="1"><Segment range="1400-1786" color="#339966" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" visible="0"><BindingSite location="1921-1946" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1921-1946" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1993-2011" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1993-2011" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1921-1946" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1921-1946" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1993-2011" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1993-2011" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" visible="0"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>9703d96c-b4bd-4f73-b8a1-dd175da16779</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="10:10:4">2020.7.10</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=Ae5/N
Ѓʒ581:-7+`*S}!sO1 |q*m,W|с_x;+
b8sHӜy@Їʸ0kD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  v    >      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="LAG16" directionality="1" translationMW="14126.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1930" maxFusedRunOn="1930" prioritize="1"><Segment range="1400-1786" color="#00ff00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1856-2185" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2036-2171" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1930" maxFusedRunOn="1930" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5485-5589" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3858-4446" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2893-3486" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2195-2794" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5415" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4624-5415" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5416-5484" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1921-1946" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1921-1946" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1993-2011" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1993-2011" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1921-1946" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1921-1946" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1993-2011" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1993-2011" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>919a0090-72fb-42c4-9571-6d529da71f72</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="12:54:7">2017.10.3</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=Ae5/N
Ѓʒ581:-7+`*S}!sO1 |q*m,W|с_x;+
b8sHӜy@Їʸ0HJ@/;44RcT۵Ј/;44RcT۵/{^?k`媪jcD<CϰCC:UNa'Sϳq<s=,ovQ{3,l53 }LL +<F6¿3Mt`P7+?}P.LH5ð|#A1#a#3PLVUU G59:R\6Vv^dE+F-сtff	M+;@FӅWA%4]/cM*Eъ?a#^_[Jλ:3;NU_{ KțWJ6⾀@-0ڼz 8HhybmVIVdial=s0C
;+0m;{T0pE4bUMT=UC̣*d;m[KD QC*S3:|S ή;s	J$,S#q?8Ϧ.	Gq4]D&$0tJR>^py&8SҟQ($	0SMs4UTon^ܥamU~FiU*0VI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
`xkx=cI}KkY"c|J;]e3ݓEU#\U(Fb_׆M}2Ut5XUPP1{gY6)Y]Y 3vT        F  v    D      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="LAG16" directionality="1" translationMW="14126.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1936" maxFusedRunOn="1936" prioritize="1"><Segment range="1400-1786" color="#00ff00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1862-2191" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2042-2177" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1936" maxFusedRunOn="1936" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5491-5595" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3864-4452" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2899-3492" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2201-2800" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5421" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4630-5421" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5422-5490" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1927-1952" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1927-1952" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1999-2017" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1999-2017" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1927-1952" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1927-1952" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1999-2017" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1999-2017" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>919a0090-72fb-42c4-9571-6d529da71f72</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="12:52:55">2017.10.3</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  #               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AeԽ8(KB*HƿN#LܮǓ5=M,J<Ɯ]ƸkT}#]F[|gL"i)"GNq{B*0BoZ,{;#?"-) TIScn֒_B TIScn֒_d??o{Ҽ>RHAU:}ĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<rďS??4A3qcleYTjET BIsڣPYxEPFӅWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj! .cMZZ&iX=*2h3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>^l%Ǧx4	tM9+H yÝPtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥV[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
N-H)MeQx!'-d1(-w|vMThr1WPn8ұ{#^6ꞭʸMUPcU@Y@pV(Yfڛ@vj&UeudڿR=        F  y    G      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="LAG16" directionality="1" translationMW="14126.27" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1939" maxFusedRunOn="1939" prioritize="1"><Segment range="1403-1789" color="#00ff00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1865-2194" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2045-2180" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1939" maxFusedRunOn="1939" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5494-5598" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3867-4455" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2902-3495" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2204-2803" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5424" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4633-5424" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5425-5493" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1930-1955" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1930-1955" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="2002-2020" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="2002-2020" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1930-1955" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1930-1955" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="2002-2020" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="2002-2020" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>919a0090-72fb-42c4-9571-6d529da71f72</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="12:52:50">2017.10.3</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  !     ?  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F      S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>919a0090-72fb-42c4-9571-6d529da71f72</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="8:41:14">2017.8.15</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       7  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="8:38:19">2017.8.15</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        /  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RFF,L;;uQDrE\`~ХP?6GпlwO<ﺱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH      S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  O<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ^<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="23:0:0">2016.10.4</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"/></Q></Feature><Feature name="LTR" type="LTR" allowSegmentOverlaps="0"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="LTR" type="LTR" allowSegmentOverlaps="0"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1447..1776" colors="white"/></TopStrand><BottomStrand><ColorRange range="1447..1776" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="933..1349" colors="red"/></TopStrand><BottomStrand><ColorRange range="933..1349" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="933" val2="1349"/><Node name="pBABED P FLAG.dna" type="DNA" seqLen="5204" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0"><Segment range="1787-2386" color="#ccffcc" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; 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   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  `<Notes>
<UUID>9703d96c-b4bd-4f73-b8a1-dd175da16779</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="10:10:32">2020.7.10</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         DSTT                                                                                                                  HO  d              E             Y?                                                                                                                                                               <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="0" name="DSTT" enzymeNames="AgeI ApaI AscI BamHI BbsI BglII BsmBI BssHII EcoRI EcoRV HindIII KpnI MfeI NcoI NdeI NheI NotI NsiI PvuI SacI SacII SalI SmaI SpeI XbaI XhoI XmaI"/></CustomEnzymeSets>
