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         #   '                  !            .      $      "   '   #                              +   &                     	            #         !                (   2            '   
      )   $   *      3      #            )   $            %         *         '   &      #   (   <                     	                                                                  
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            #      ,                               $            -                   %   "                                        !      "   4   &   ,      3   +                                    &   )   !   +         	               #      %      )      (      7   1            .   
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TZ%>+j!%0# & q U4		


(2}O3`( 4BGT0a
>SVx@,7IF =/< 3%xF !`}-rw?B+6GHDEwx	.  yuNTfp~.sCg 3
  _ &"! k`dLy  zgt	/hz!a 
 U 0|vqp

ta<
z <OUIuDHSR  ;x~	48h	 ,3
  D M `  '#7	
!PYl az  )r("6KvM7H   ? P%J	$KAbe7  I X  4~Vw[			?

0k#
Udi=C  KfuY	s

B{"F  	{/C
;#3  Wha	f
v


h4bao Y(oc { 1}lr
pu=P   V f Aq
 79'brBD w,E` \=PVhT ;PEdwP  R=
Jv|I^ % 	
_S o]l   Ve<c

?
{5i6  h .~L	i)t2$9xMV x)sys	r@OY=Gf) ?4
:
 cin	A`u_
 -d

 K
?6'  E N a  (:		
"QZmX{ =]$.9^			GI~b q		5[2b!# 8	

F `N=	

#lK[|I  gsZ	r4 *)
O
bN8] G4
7LwS9<  QK:Gy	
		HUi9YRBti   Sa\	%lof^c  !&DQs X	.
<t'  @pY	
 7"DcL|c8D  Y B	@

1~eSn% J MWxg		D					k


]$V! " m  #
M.yL MnwQ.>~! ! 5v\/	


l0TjW)D bhOXP	e	wn&Gn%r   S   :#LRv1

_|1# Pt

CWK  ^Lg;	Z	
p 4jsVz%   k  1
v)@02i	
!
,w$XZx u{|rD<+>4  x	X
% V2" 37?Fb{	R
_
]  X^O4[	g
y'**  i+X
?6k	
pi5t\4MA "Bf&eiA	b		|

.T.cp 0 {   O#
w
l0<Yb3 >d-@  *d CiZ)pm| muFz	!Pq % p am

1|Yi$k 2~$t=Rqv>fF2<K stGygRyQ  q+I5J6  W JVBr
cEB 
8AC  :O~]O  g w-}((s> 1#w<5  \~-			
Fa f
aZ\ ox-
6{;] L


KLP(  ]"NKQW iV$ >azQ
^
U A5sS)X
 l *H`
<QFXexfQx  ,U'N};4A 	
`:\Y[#  @K!4J
]

J~( T)G&	
M|~_:Z, S>?n Z
[
\
KLwxyz{}OPQRS\]z{|}sY   Eo
T^t_j& Fl	d-"	9G?p
/ po

/kP`  
s9L
'`+UTuk  , w  ,+	
W{
P8_' IbG03	e]U
:@ }m
L^m0 pD> f	T
".e#rHq$ 9    =
 E#5lj{Q .fp

:@6 T		a
d


W;_ EWq2gQ0|%	7a-  i  /5y8dtMM	j|*u,%,Tvl  AH<
+
(:M _lmLy-VTW NW }:
ac
3yU  X94t	9NXcPdiZ>g   y*tu,z	`LDH*% - x U	68	

|-Q9a1"  %-"I		s,A1( Jk{&	5
;
cVw'\ 1I4		fRA  cH-;t  5  @E_U		{;^A\, );M~<Q]	_k 5v" S2nT`	, Z;djpMnf1 
o	~		

2jBPar s ep		i
K? D0i q
+QZEih ` 6].6	
J[a!! Y[9	$		)@I$&O {L
6%G]&- " I	U
((Iv)k4 OgZ0Bv		/;DPZe#&/;BW3f7;L[sk{@r&  : b    \;			&

q#Fn=|, F    7>^	
  R( ) t  R)T5^67YkJu/,3  O   %k,-L				


 I[$72m|GRe+ @   g^	q%	+		

r@v'LN  ^ wA/_| 
jA*5_cv  /{:	H
;7`	 >($
#J6G }UvZ.X			e

jg3:"<` +6X'nbO 

^cv$  nU r		b
?\kzFeU cm	>@_&^ <89	EZH1}" F3
N


R8J; CXrRFGh8Xnh
sCb&  u"	C			


	/S}VRm}m)  R  aO9g:O	d	0

vo3^mr0UJ!  j  06zu(	W
$+v*W\w!+   / z !]ehNNWd>Z@		{
ko;-x&-3L  Bht=y	)c  p#4Is;	 I
vN [		
,
5 `MP

 UO  +W~eX D(Fm%I	

 9rX^# 	8Kn*k	~

cz*.>	O H2	
!dT=% 8   -DS1	
~D9P$4z: x~;l
x+SV) $W3!58Mb,0chKC 4  jDHzT	Q:,@v[[( (Y	:RL1;	}	
o1jiOS4eq!+ r5	
IZ~ Ch/h7 /X8zH	u		~.:COYd%(:V5H$:FNjuj?,1    Q$[+K	]				%
Sp]1<6It+d  ? ]    z#v{
?Ki	M_F  Tu-&>]ifmaEd ;Qb
=DYWm?Gg:5.  . y   \t!c9Y?				/
z
u.R2:q2
 "x	3H u  'E $7	 

}='  CV2R
 0
 }+ObB1 3 ~ 'iq.y0
n-B9Gh#+9EN?3u}gp*$   :ZJ	

=R\h>>eF9E'   B#/b		y
t2GNp3 2 }   &A
1Fg8=d>2Mmnodef)  0F  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                     :                                                                                                                                                                                                                                                                                                            (                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            	                                                                           	   	      #                           -                                              
                                                                                         
                                                                                                                         	   
               %                        
         4   .   
                  	                     	                     !   	   	         
                                 !   7       	      (         .            @   
   *      '         U                  :                                                                                                                                          (                                                                                                                                                                                                                                                                                                          	                           	 	  #         -                
                                 
                                             	 
     %        
   4 . 
      	       	       ! 	 	   
           ! 7   	  (   .    @ 
 *  '   U   ra  t!  t?  t  y]  ~  J
				 3 4 ~   BC87;		/
~d:p?@NO 1C  ) t ^		5		_	k$e	p|my*	s47	
hC( s 	47( s #GPN	c		M
}

	<'4=4/lP		k
N	
kk

<..<Nu&			GhCGN		zRt		NTR6 fN	 u7  Ck
eR  	
	eL		 	
<	(
	*
PX	7my MmA@D1.Yyqx`! 5E
{Tj,);0OcM@
	1_5YW!g*ij		6[@j	[@'Hi3TuEwPl Cj	[@! Tjq*7iD6Cl=M@1Y	,v^R  h*M@1JY  MmNA@qD
1CN.Yy?fq	C		
MN^	c2 }   iZTu%

i?p>u
PCS	9%E{,J	Q*E{Pr,a<Tui	 *7Cij		D6C[@|! Tu
i?lx#[xC>	S			F&8%0UTui29!Jqg'.Jiu	t	9NXci>*i6 e &q	C		
0O 

ed@	-
h	j	*
[J@ 8L		

(P 
T/CS	O L	[$uj   hO L	[MWL9		QXCn| 

> (Jq
grqNX( [t!		%
.Rp<|b	<N^[	 gj,V

nH6wF < Z     w`		
S<Hf/3 ~ ijz+?uv[pWqg	a   		
S/ j,

H6FgVnw >EdH  3>

"	T?	
. Wgj7,V^	

z}$'n4H6Mw++F3 ~  ij[t!z		%
.Rp+?uv<[mjyT `	u

n	

|QS]6It+2=
r	?Fe8   D4zagO	d	vm7
H
2 H		+(#HYBX[8   agO	d	vDm4zk/

JJ
5_\5`.y-B9G#9E%	b&czwF		o}`.
J[ X980=3	ed@T7=kD
&		

f9[y5$		my- NczF	o	
fyw	}W7^	z}$'4M++

k/Ju
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L^m0  !     ?  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F      S   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="11:25:21">2022.12.8</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       7  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="8:38:19">2017.8.15</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        /  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RFF,L;;uQDrE\`~ХP?6GпlwO<ﺱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH      S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  ^<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="23:0:0">2016.10.4</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"/></Q></Feature><Feature recentID="8" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3994-4587" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4959-5547" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6586-6690" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2938" maxFusedRunOn="2938" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="2" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3137-3272" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2957-3286" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3022-3047" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3022-3047" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="3094-3112" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="3094-3112" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3022-3047" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3022-3047" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3094-3112" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="3094-3112" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>01e2ac08-5ca7-472d-824b-326f9be0452b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="11:25:21">2022.12.8</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         DSTT                                                                                                                  HO  d              E             Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="0" name="DSTT" enzymeNames="ApaI BamHI BbsI BglII BsmBI EcoRI HindIII KpnI MfeI NcoI NheI NotI PvuI SalI XhoI"/></CustomEnzymeSets>
