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"'+| D		(
-Ev_&tI	{ 7
8&DH`+ * u  j*C)		
Jzgp2^tN'7uKSZ
 Sd/	b<Mlr(l %#<>`'8OZC& ' r  &l	
S[Ueh%0Q! P +nv`J		
/~J9-.>   8V-nZ		

n"!K(  h_n	R	i
PvT4n
'}]px  A  5b		T


&"PV{*u$I 	'
D~7kGk# & q UmY		
RZ,d=# 4 	}3Umz	&#|o8 3%D|		OTkl$%]^!"xyz{TUblmn  	
hP D_c  _ &5E}	>
RHhz=A)V  +?		
iqAfvEW>d 
 U 0|	
Q>eWxu <!n
R!%0/  
[`E ,Fc	Y	%acc  D M `  '		$
?

A-6Ix>W  )r~
S(Se*%   ? P%J69u(i{?wBg   I X  4~V,@NW
BH 2A}F   KfG	&	=	V			
3wX#  	zSx

p



g   WU"		Yjr`E?>Le B
L@sXy 1}l	
R

MRf-v   V f 
?O!  \=C		3
9E}1o u"ATm-  R=	
	:
S\Y^&;f "go
-
<0p ou
.:pI\ai{    Ve^	@	!

nXF  h .~LX	QU^*3 x)s,az^6o$C ?Z	
4} cidO	
&3=R< -$0xd G	,b"^dep$  E N a  (O	

 X-@.7Jy5uX =]W%


$B&[? e		@
8n?    
# `Ni
TI(8Y&  gsZ96Of *+: G4gp)G)T0~c" QKz)A~P%2F6/QF$   S		
>zVILC;h@  !&DQs:m	
^Q.  @pY7vw	

6D!@~)Yj|@x!  Y M	a
@;|[B0K2 J MW2Ab.Rt				
*
K

G`{czv3) " m  OX
[

H*V~)*KT.[) ! 5v-E	b			e
CXI1G4u!' b=H	'	3	}				5
x|Kp$K^tO  S   :ksW		N_x<Y  P~E>	
s
 4(&  ^LgM'k		
4VN\MGP3|W(   k  1
v\^`Z`	q


;ahjxz	T57U u{
k(^}  x{98	
^
/]3h 37?Fb{T y

tla:  X^;I	axV^'  i#	L		
ks aMFqwQ9a* "Bf5k
8=j1@qM" 0 {   OvV\p	Zhdx6k?f >C
sA

  *A CiaMtJYz mu;m-N % p am	S

Y6~FHw 2~$=
gNSgC#(  
Q$VD/V.  q+I8	'd  W JVwo

2_@" 
   9
8[]:|e,  g w-}(-P`]h T #
w!2o \ 	

>t
 fo7b9 oX: LD	
`
:(~)-   ]}r<	4

MFo3 >az[_	
j\2g A	
pvPw0`w5]jp lr` *H`<
#5BUC.U  ,U'1,Z^\\ "n
v =n968&  @K
;^		
~

Li'v[_\ T)G&!;
Y][_<7% S>?nTUVWXZ,-./09:WXYZP6   Eo	
|KT1;gQ<G F	
L
$hM p&h:	.

Hl-='  
s9#p
3U=21RqH# , w  ,?Ec+	
:}4X-`< IbG0	v

teq:2] ~
6);kJb p\g!ji| f/vMB O%N2 9    'i4_	/


+'In"m nIGX. .f"

oie TV	;	A	m<n EWq2g
D\.Y>5  i  /5y8dtMKi1	VzYR		1_SrI  AH<	m
c* _l$Y=
Vn
3m14+4! }:q	4>@dV2! X94tQ~+5@-A{F_7D   y*"@,	
;fb=)tp!%& - x U	6s+Fd{

eY
.a>!  %-"r		m Jk'm@f3Ti9 1IK	w
/q  cH[	5
sa{Q# 5  @ESH	
T~ ur;|i^9	  );M+'Hej<HS S=P	1x=	 Z;dj
s*KC\ 
e
74lG->}cO} s~%C	^r]y(h\} z!F q1R.7"FjeE ` 6].c%'c8>ek# Y[9|cq_a&eg,q {		
w->#$:s`
i' H	H
f&SH6 OgZ0Bv	0N!-7Bp 4Cb)8PHXO2  : b    \j5		(XAJN #KnvY1 F    (Py`e		0


!OYch/zoju% ) t  R)B.	
,u%o1];s6H'R	7  O   %k,$_	


"._&8JgY$/B& @   g^X		&
h
%CQOuS)tj+cj  ^ 	G\<@Sak  /{ 	
p@d= >($#


@  'bvbf$ }Uy%	<			

XGD= A
Q

K?r, f		B		;@Sno"  nUW]	m9oHyWn#zB2 N	3|/]<; <V

"7%Z F3hF/'+ CXrRy	

=yU$E5KEP ?1  u1-Ls			J

?GW0Z3/JZJ*  R  aO9Lj2	|	U[L{;JO2{s'$  j  06zuY
:`S4`9T+   / z !]ehN:u4HjK	o	
gLc
U
)  Bht=:@  p#7	f&P Ivn
7gSd+ [	
	1s `Zq
on2,  +m
4[B5 D(Fm% }


O5;  	8%>b
9W, H}5	sjA1) 8   -DS1f3	*H[!l-hWd x~;J<hyUm03. $W3!5rq	
*?	le@E( # 4  jDHRl.p	hS`z88" (Y	:RL*GiGF,0B|~N r5B]Q&7d[ EE= /X8zu{		G

p ,6Ao3%#+GRG	h:    Q$[+#A^x	-	
NW0Mg:&QA  ? ]    z#		?P(Fsca*u<i#  Tx\

W:FCJ>q"mA# ;Qx

<2{!64J$D0  . y   \t09t,GXn	r	{		fF_/bNz "u
%R"  'E $7p


8Z)  CV2R
 0;e|
rxZ,?l6 3 ~ 'iq.y	\Fk
$Eo "+gRZ}DMy'   :Zw	M/9EB#"&   B#/8tz	

7$+kM: 2 }   &Asx	y	

Ej#DnAf*JKLABC  2  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        @                                                 	                                                                                                                                                                                                                                                             "                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                            
                                        	         /                           6                                                                                          $                 
                             
                                        "                                          	                                     	   	   "            /                     
            5   8                                       	         	               &   	   	                     	                     #   =   '      !   ,      "   6   #         G      '      )   %      g                     @                     	                                                                                                                       "                                                                                                                                                                                                                                                                                                    
          
              	   /         6                                $       
           
              "                	             	 	 "    /       
    5 8             	   	     & 	 	       	       # = '  ! ,  " 6 #   G  '  ) %  g   z  |  |  }h      dx?y 3 4 ~   BC879xG	-			?fAMnggh+,|a ~}{F w) t BO]_HpYmV^MPnNk l( s \7( s #G		|
hn`~\I-ixW(k~}N(	kkyXFzr	(+R(m$ $+r	);	/tr	)+;	1/{	  7  2 k
i	LB/V (
A,	t
A)+~+ (A	)-^m	mV` MmA@6VN=c! 5E	T1|G^	;mj%,M@<64g*i88'Hi}Fk	1R"T- C8! 	1Gq*7g! IM@6		v_>	/  hsM@`'6  MmNA@q(Nu	AB +6VCN-		J
enhN>
#w2 }   iZT*	]2Gjkl{FirMnfR-_?nC,[E|	K>.K	E|-O	><TF *7Cg!i 8;	 TF<U#[xC]	#2bT]2{F	Jqt$'ikR;
t+5@F* e N-j		J
%e, ge

m"	jEa	^z)8' i~;	'4\-fm1{t/CO $G
8RGh~s}  hO 8}4hn
)(.X 	a
K`  Jqr+5 [t0,	r		FW/M|?c<N>
 gj3J		
!K^%Tf# < Z     w)Ou	BK|0%Cp3 ~ ijklogRSy8i	L\MWqD>   )Ou	B|0 j	
!^%f#g3J	KT \"AH  3MV
1|?
}c Wgj73G	J			F


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6);kJb  !     ?  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F      S   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="15:54:39">2022.1.7</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       7  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="8:38:19">2017.8.15</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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7jH      S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  O<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ^<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="23:0:0">2016.10.4</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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fG'כx^uUDf,bˮI4;f\iVaǺts>1s\2L0ht0vGFi<&;b%?7'CLIP       	           <<?xml version="1.0"?><HistoryTree><Node name="pBABED P FLAG.dna" type="DNA" seqLen="7446" ID="3" circular="1" operation="replace"><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="1366..3678" colors="red"/></TopStrand><BottomStrand><ColorRange range="1366..3678" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="1366" val2="1436"/><Node name="pBABED P FLAG.dna" type="DNA" seqLen="5204" ID="2" circular="1" resurrectable="1" operation="replace"><Features><Feature name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; 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  <AlignableSequences trimStringency="Medium"><Sequence name="194_44-pBABE Fwd-291118-10-43" ID="0" isTrace="1" sortOrder="0" trimmedRange="0..1059"/><Sequence name="194_44-pBABE Rev-291118-10-44" ID="1" isTrace="1" sortOrder="1" trimmedRange="0..985"/></AlignableSequences>  -      BC N sre```pp23*+/*IMbqqQjZjQj^r*, >6         BC ;,E% JЖ $l90{`3m9-..&d5Rgگ}߯߼>^/?~,~x#$&׌"4{dgCy$xOT3ndt<}V:uGִ&,ז5v Cqߪ}t[DH6YH^}y91Am]{sTL진4ےblI_6|*OXǕ޳f	Q9#Zng9q^zOeN2n_G'9l%㜋nOc5
ntc"ϴa:jE]|P+A}፠AYԢ9&OmLO÷s)
!L=T==%C~|^70vx$ ns]D
!Sޙ&i7+,l YÍOṤK;LOXDjJs9f|:B %iK*R[֊C~PBLU Nb9n+@=CߺNo5UJ]&K=	`#A
)5O'U^C,T:K<z`[XP}X>ɽz|٤U.*e=У\Epv&3pB̀2[{R?Q        BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3870-4005" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3690-4019" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3755-3780" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3755-3780" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="3827-3845" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="3827-3845" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3755-3780" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3755-3780" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3827-3845" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="3827-3845" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="15:59:41">2022.1.7</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         DSTT                                                                                                                  HO  d             E             Y?                                                                                                                                                               <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="0" name="DSTT" enzymeNames="AgeI ApaI AscI BamHI BbsI BglII BmgBI BsmBI BssHII EcoRI EcoRV HindIII KpnI MfeI NcoI NdeI NheI NotI NsiI PvuI SacI SacII SalI SmaI SpeI SphI XbaI XhoI XmaI"/></CustomEnzymeSets>
