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zO2I <T
S  	 	 ,EWT}  D M `  'PIE  )rK


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]5c   ? P%JO	%
^SK   I X  4~V	,	w			{W";  Kf@Iy

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T_q,  Ww2
AJ-%

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KjlWz
 g		
'C oo0    Ve 	
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N&9M x)sgp


1j ?0	s.}B>H& ci+`)X9U -.
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aeZv Qk	m			'F$ `Ng#	3
L

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R+6 *.$q,d G4o@m^#5; QKSdqwqew-R   S &

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@
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N
v
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mw0Q} P	
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v$*Riu%	
ex'tu u{M	+`."  x
dU2^r- 37?Fb{b		{'f  X^
w.FXT&'  imw		
BKRO\w "Bf]	X		


iY4n0 0 {   Ox3bTz 6b			
3	`zi: >J-b	  nF0 CiV1	 mu;Q] % p amqr	@#f 2~$#
/h
 yN  q+IODd	H4  W JV1?P+	v	
@^:$ -  Y &!
KNcy  g w-}(f
d/|Z	 _|A8= \S8	j	|		j`ldu ff4: o=]T5pwIdQ L[#	
A28#o6w  ]S			
Ov6/P| >az#&ste A	QN39
 lUP *H`?eg  ,U'
~.{MYb {	\oHc  @K"		
@P1M 5n5{ T)G& fX% S>?n!=>?OL<mCD   EoV
BWfk>=E F	
fR p0h[	

nvg@DF  
s9g	 

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W
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piKS 
U p	OQ+!/2 f}%g.{6B1' 9    1	\	

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CG T	

hsAqt7j EWq2		1
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@FHN  AH<;dh	]D? _l
d*t:7= }:		


O	,AyY'h X94hq
G m-a}J   y*tq=
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<5C3[2@!  %-"
r


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(Ls'q  cHt7=a0Lav~ fL' 5  @Es1	4	
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VY Z;dj:V 

D
,|"o$c*+" s			g

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3 ` 6]./
S
sX)0A Y[9&5v)I
gT {~xP j/|'/7CHVlMr,3 OgZ0BU	y~2Ih+7KyG2V,  : b    \Z9:		a

*6ar~['8K]! F    A
Z
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?QV/  O   %k,"I'	]	b	*

3Z{5<[g;>F( @   g^6UyB	H	~	
l6Q}e*Z  ^ jI
DTpy  /{			R	^H >($N	]RSD }Uv HI`zVtfpk p	b[8w f		
JiBuV  nUo>	

MNLr -
e
rG(ap*G% <s}l	n			(
Y F3"	2
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

M
u

A>E7DO%  R  aO9A4

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*w1]z5   / z !]ehNal
5av			


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<Ne4;eB# 	8%/Z	f		;

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q

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ej${!n#b. r5	
	r W(6 /X8zi}T	 4u6HOx.k~fS3    Q$[+!@Y8a	Y
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A	G	M	H

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>

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9Z|63A*  CV2R
 0 (		
{EU9D4O2 3 ~ 'iq.yE|	B

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s
Y8uCNf: 2 }   &AVWA

'0oxMX$'bAB&Slm  0  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                          =                                                                                                                                                                                                                                                                                                                *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
      %                           2                                              
                                            &                 
                             	                                                                                    
                                        	   !      	      &         	            	            ;   5   
               
                        	            	            
            
         	                           6   %   
   "   '         3            ?      '      *         ^                       =                                                                                                                                              *                                                                                                                                                                                                                                                                                                                                         
  %         2                
                &       
           	                                
              	 !  	  &   	    	    ; 5 
     
        	    	    
    
   	         6 % 
 " '   3    ?  '  *   ^   p  r}  r  s  w  }  		 3 4 ~   BC87?Er=
 t&(!6VWnk#Qp"	9 ) t >_P
zpm+3( s C7( s #G	Co3`#``
	k#Qp"lNkkp		%	)qV
t	 
9
 
97  (
_ 0g=
w
-
<< 0g:E}c3	m MmA@x	01^
b^n}! 5E
|!;S
A/Z	e
M@0}?>g-
*\	m'Hi*H
9 Sy	2_! q*7n
PM@0
HvtrT  h7		*kM@0%8  MmNA@qx	o
,t01$^QzM>
|R		(*?wN6
Aje
2 }   iZTXWA	

s

(pHq4-Zd6C	qJ	]Cw(y5BE
!`	1yE
!L+<T *7Sy\		n
2_m	sj TA	Hd6)<#[xC_{x		
5H}s(459\Tq4HJq8
$/		i4f}* e A>/Z	
| 3{<;e

JBXcB *+	X
)@Dt)s}j ma/CO iI
4[i~#h  h	O I
R		>(Ic,XKQ	h x	

 DJq
$r
0i [tzY8X	
`
t

)`q=Z7D|+@xg<N6
Aj gjku&			
}DV:=Z < Z     w]1	Cr$1hQ3 ~ ijE
-<	Wq	ouu   1C$1h j&	:=gku		
}DVZ =MKH  3	!?0	.	 Wgj7ku&	3			


3 d}DVYj:=Z'3 ~  ij[tzEY8X	
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t


)`q-=Z7D<mnL 
,L
6CQU2w

o
8   
:Oa4

 gG %m%(tg#	sq}8   a4


 g:GOu2				QV"	2
;5B	K_\R@.y|	t5Nk

Z]cn?"`.,S
|)0 X980@
wP FmaH7e		:
9ZC+Nz		0
tCG(b	P

-
*=cn?"+	0
t(W73	

3 dYj'u2"					2
;5BQV

g@&4
Y[9&5v)I
gT/X8zi}T	 4u6HOx.k~fS 3	k


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U  !     ?  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F      S   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1513-1538" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="1513-1538" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="1585-1603" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="1585-1603" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="10:1:22">2019.10.17</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
       7  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
`J63 D0ɠξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH        F  S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="5" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="10" name="FLAG" directionality="1" translationMW="1012.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1522" maxFusedRunOn="1522" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5077-5181" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1628-1763" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1777" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3450-4038" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2485-3078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="PuroR" directionality="1" translationMW="21532.30" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1787-2386" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5007" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4216-5007" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5008-5076" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  _<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="8:38:19">2017.8.15</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        /  T        TOWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2M.*j.i{RFF,L;;uQDrE\`~ХP?6GпlwO<ﺱ>{n=AekD< XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5Tu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|?36O>q?eQjKP XT߉
S%jC`UgeDRmB]N`=]n Ҿ?ôQt`]8YtSJ=EтF0?ҮD_m#AV>볮+3:eQ?u,	e4o.+! .Ǣ.L ݍ6&ij
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ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2퓷8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR%mnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH      S  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  ^<Notes>
<UUID>73a0567b-5391-4dbc-85d2-b5f6eff47d8c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="23:0:0">2016.10.4</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="TRAF4" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1400-2812" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKPGTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS*"/></Q></Feature><Feature recentID="10" name="AmpR" directionality="2" translationMW="31543.33" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6390" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5599-6390" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6391-6459" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="9" name="PuroR" directionality="1" translationMW="21531.55" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3170-3769" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin N-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA*"/></Q></Feature><Feature recentID="8" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3868-4461" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4833-5421" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6460-6564" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2812" maxFusedRunOn="2812" detectionMode="exactProteinMatch"><Segment range="1370-1393" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="2" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="934-1350" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3011-3146" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2831-3160" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RT8289" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2896-2921" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="2896-2921" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="2968-2986" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="2968-2986" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="2896-2921" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="2896-2921" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="2968-2986" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="51"><Component bases="CCACACC"/><Component hybridizedRange="2968-2986" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="2452" sequence="CCTCCTCTTCCTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite><BindingSite simplified="1" location="1248-1265" boundStrand="0" annealedBases="CCTCCTCTTCCTCCATCC" meltingTemperature="54"><Component hybridizedRange="1248-1265" bases="CCTCCTCTTCCTCCATCC"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>6a58cf29-5dae-4622-a877-8b7009d50d21</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="23:0:0">2016.10.4</Created>
<LastModified UTC="10:1:35">2019.10.17</LastModified>
<CreatedBy>MRC PPU</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         DSTT                                                                                                                  HO  d              E             Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="0" name="DSTT" enzymeNames="AgeI ApaI BamHI BbsI BglII BsmBI BssHII EcoRI EcoRV HindIII KpnI MfeI NcoI NdeI NheI NotI NsiI PvuI SacI SacII SalI SmaI SpeI XbaI XhoI XmaI"/></CustomEnzymeSets>
