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)0Z|D 0;MJA3/ 3%Uv{CF/:KLHI"#{|2  ViwGk$7  _ &f$odhP}  &l~%e 
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h  D M `  'p		 
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"	

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Ol8fes d,sg$ 1}natyAT   V f 	+fvFH l \=klX Ih{T  R=zMb!) @>cW w		#
V
ap   ^my|G	


C9m:  h .~TH	-x6(	=|QZ x&1QL	

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 l	U	

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&U^q	\ =na						
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		!

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v8 *[		
<R<a G<

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LYm=]VFxm!%   }	E	?
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@x2  @paF*	f		P


";&HgP"g<H  Y {	iWr J MY	

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IQ2}P$Qr{U2B !(=~t	p4Xn[-H b\s&~

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~0{(\^| u{+'
"Cs@/B8  8$Z6& 37?Fb dz6ma  X^}+."  iq!3
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i

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:  W JVW!$N
gIF 	EG  ?a S  g w-}0	,wB5'{@9 -<k6	
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z
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]mZ( >alY AwW-\ l 2PhJ\i| jU|  ,U/> ?8E d>`]_  @KN", T1O.c>^$ S>?v{|}~STUVW`a~w]   EwXbxcn* Fx	
"A`1&=KCt3 x'u:s	N		
3oTd  
{A	K	d!/YXyo  , w  ,i?[T<c+ IbG8W


aaY>D #Sy			$
#BPbq4 x
HB f9
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
>D : \z(	"?c E_y:;

kU4);e(  i  /5y8d|.y"0)0Xzp  AHDU	L>Q _t	I	L	}1ZX
[R[  B
eg 7}Y% X94|$	


8=R\gThm^Bk    y23:	
NdPHL.' - x U'>fM	
@1U=e5$  %-*RjYT0E5	, Jk?
bgZ{+` 9#

VE  cH\1 ?x5 5  HM/4ad]i~Y		
+
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jQrj5( 
MVm`		V				=
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d

OC cH4m y^/U^Iml ` 6]68		N_e%  Y[Ai	
-DM(*S" B		:)Ka*1$" np},,Mz-o#> OgZ0JJ7&	k	
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3?HT^i '*3?F[7j;?P_woDv%  : b    d<	W u'JrA$ F    #QM	J
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,"  ) t  R)$
pX9b:;]oNy303  O   %ks4u		.
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

ovDz+PR  ^ 
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s

P'!N:K" r)
		)		


Gn"k7>&@d
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LC`o~JiY 	BDc*b <m				

I^L5& F;hA
w

V<N?- K`zE <D		O

!Kl<\rlwGf"  }OX			
3WZVqq*  R  aO9;SYr	}

s7bqv4YN$  j  06z}c7
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k

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 IV	MzR ;Yj5	_		C
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9 	 `	\YS  +=[i\ D0Nu-=v\b+ 	@ht		J	M		
!~ .2BS H"8


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" 4  jL>}

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M^$Gl3l= /X@I%	h		



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VWta5@: Mx/h   ? ]    z+4
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g A+  CV2Z!(8!R$/SfF; 3 ~ 'iq.HTl,	-	g	b
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a

5Jk<AhB6Qqrshij-  1  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        @                   	                                                                                                                                                                                                                                                                                            &                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  	                                  	                                         	         +                           6                                               	                                                             	                                     
                                           	                                                                       	         	      &         
                        <   4            
                  	                                 !                                                    =   !      &   /         7   "   "      C      "      +   )      f                     @         	                                                                                                                                    &                                                                                                                                                                                                                                                                                                                	            	               	   +         6                 	                       	             
                 	                         	   	  &   
        < 4    
      	           !                  = !  & /   7 " "  C  "  + )  f   um  w#  wC  w  |    }n	 3 4 ~   BC@?
	,h	
Ah>tCDRS#$5G<	 ) t M9_
o	pmk}w8;G( s 8?( s #w@+8A!83pTP`
V<	
@22@Ry1o	*KGKRxV|R
XV : q |

7  qGiV 
P:	:	 7,
T;m} MmIH5E52]}u d!(=Mtdi	yXn0-;;SY	4SMH55c9]l[%g*
:_D_D'Hil,7XyI{T" F	_D!(tXn
*7H:GpAMH55]	0v
V  hp .MlH55N]R  MmNIH 05E_

5GR2]}Cju	
V<2 }   ib4m	->m			UmCtBy
TK=)g1y0U2
yTv0e	DZ	m *7F	
H:G_D
C% 4	mC|#[K0
7
J*<)4Y	m6=%R

+2Niy$	

=R\gmB*: e *u;SY		
4S G
eh

1l9d	
_ND x


BaTX8N7KO _(yn"l	 $
  hp O _[#PU`
Gr	
C 
B 

%

R\, c|N;			V2Vt@f	DVZ gjor	-
4
g
j
H{rL:{J < Z      _

W@Lj"33 ~ ij>
Z/Cyz_Z$q		

]st_y;ke   
W3 jr	-
4
H{L:Jgog
j
r{ wJF	T
BIhH  ;9&XG.3`\hX		 
*
]
 2 Wgjor?c	-
4
g
j

H{x{(+r8L:Q{//J3 ~  ijc|>N;			
ZV2Vt/Cyz@_u		
Kn}X rQ
Wa:Mx/:$dPK	LCJi8   "H8~ar}

q /(L]F\_8   a"r}

Hq8~po3Nn9_\
d.Hg	1F=K'=If*c
=s`6m`V	[
	N_ X988
8hDX!?
g A=[0		*
j=_}9		16	I	L	}1
Rcs

j}=W?c
x{(+8Q//po3N	4f&'4kY[Ai	
-DM(*S"/X@I%	h		



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	s2PhJ\i| jU|P  +nv!.5LYeq{=aWu5Vb7	p					
6
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}]q `;&2ET@Uxe ;#Sy			$
#BPbq4  'J               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2LM.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n`A]mA+c:#vq+1=N!?oҶ'7yS@R3!p AￍJ۝B0^?cE}'A?4C8Ppq~uQOӏ>u](qI{6&M@ ?op%pRȼ11~p|58}24aM7Լ?9LnpםG<B@ͱ8P6ǭAvu8u]=.f<u4QpPQ]24M?_yM/?S1`8<{N'R{T'Ĺ?*WxJ==P{eXAZ6͢$!@ŬBǳ0"-) TIScn֒_B TIScn֒_d??o{Ҽ>RHAU:}ĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S??4A3qcleYTjET BIsڣPYxEPFӅWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj! .cMZZ&iX=*2h3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>^l%Ǧx4	tM9+H yÝPtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥV[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
N-H)MeQx!'-d1(-w|vMThr1WPn8ұ{#^6ꞭʸMUPcU@Y@pV(Yfڛ@vj&UeudڿR=  R  !  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5075-5663" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4110-4703" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3412-4011" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; 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  B<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pBabe Rev" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3138-3163" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3138-3163" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="3210-3228" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3210-3228" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3138-3163" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3138-3163" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3210-3228" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3210-3228" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="pBabe Fwd" sequence="CCTCCTCTTCTTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite><BindingSite simplified="1" location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>2f363923-c680-4757-ae7f-cab164944e27</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBabeD puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="23:0:0">2014.4.3</Created>
<LastModified UTC="12:6:58">2021.5.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  "               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2LM.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n`A]mA+c:#vq+1=N!?oҶ'7yS@R3!p AￍJ۝B0^?cE}'A?4C8Ppq~uQOӏ>u](qI{6&M@ ?op%pRȼ11~p|58}24aM7Լ?9LnpםG<B@ͱ8P6ǭAvu8u]=.f<u4QpPQ]24M?_yM/?S1`8<{N'R{T'Ĺ?*WxJ==P{eXAZ6͢$!@ŬBǳ0"-) TIScn֒_B TIScn֒_d??o{Ҽ>RHAU:}ĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S??4A3qcleYTjET BIsڣPYxEPFӅWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj! .cMZZ&iX=*2h3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>^l%Ǧx4	tM9+H yÝPtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥV[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
N-H)MeQx!'-d1(-w|vMThr1WPn8ұ{#^6ꞭʸMUPcU@Y@pV(Yfڛ@vj&UeudڿR=  R  !  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="mCherry" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1363-2082" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6632" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5841-6632" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6633-6701" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="8" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3412-4011" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4110-4703" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5075-5663" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="942-1358" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3073-3402" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3253-3388" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6702-6806" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  B<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pBabe Rev" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3138-3163" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3138-3163" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="3210-3228" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3210-3228" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3138-3163" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3138-3163" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3210-3228" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3210-3228" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="pBabe Fwd" sequence="CCTCCTCTTCTTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite><BindingSite simplified="1" location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>9c128e4c-85f0-490f-a6a5-155c96dcc7bf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBabeD puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="23:0:0">2014.4.3</Created>
<LastModified UTC="12:6:27">2021.5.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  "               OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2LM.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{n`A]mA+c:#vq+1=N!?oҶ'7yS@R3!p AￍJ۝B0^?cE}'A?4C8Ppq~uQOӏ>u](qI{6 oZ,{;#:b"Ґ"KM66i-5"KM66i-5K^+?8yj>9E$S|I3r@s2ǟwݱ0-8^B ,0s,S 0̓ġ0Ã]>3A?2
=.@<;03<?>~?~q0:-,B?3~5?sL|T76UF.U@aQ~$*NN=5UYJѵAt`]8YtSJ=Eсtff	M+;@FJ}b@XjҳθD3Ƈ'&ҍz 8.L .>@-0v4؛U*Rjv(X[\£&;:;x{8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqW)@jFUAߑZUaJ<Rfa%zJ}c71+odȑgۃ#a1FY_OOש斊<9RJJY*3\hCl-*	;¿\mCآ5U0/lIJ4m킘46Z-o%긇{_RH2pgdzHW+{+7nL]V<TwbiM
ha0gfbjEVWV@+ݫU#7  R  '  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="mCherry" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1363-2088" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5702" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4911-5702" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5703-5771" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="8" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2482-3081" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3180-3773" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4145-4733" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="5" name="LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="942-1358" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2143-2472" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2323-2458" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5772-5876" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  B<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pBabe Rev" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2208-2233" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="2208-2233" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="2280-2298" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="2280-2298" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="2208-2233" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="2208-2233" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="2280-2298" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="2280-2298" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="pBabe Fwd" sequence="CCTCCTCTTCTTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite><BindingSite simplified="1" location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>9c128e4c-85f0-490f-a6a5-155c96dcc7bf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBabeD puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="23:0:0">2014.4.3</Created>
<LastModified UTC="11:57:2">2021.5.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
         !  ;         ;OWA%4]N`=]n Ҿ?ôQt`ь/O4F*P~y}m+:θ;YTO=/hxK?"oY_(ǢHhyj ;/|}צ./.H	&Į5@z*,bA$_tϼZ _2LM.*j.i{RBF,L;uQDrE\`~ХP?6GпlwOﲱ>{oZ,{;#:b"Ґ"KM66i-5"KM66i-5K^+?8yj>9E$S|I3r@s2ǟwݱ0-8^B ,0s,S 0̓ġ0Ã]>3A?2
=.@<;03<?>~?~q0:-,B?3~5?sL|T76UF.U@aQ~$*NN=5UYJѵAt`]8YtSJ=Eсtff	M+;@FJ}b@XjҳθD3Ƈ'&ҍz 8.L .>@-0v4؛U*Rjv(X[\£&;:;x{8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqW)@jFUAߑZUaJ<Rfa%zJ}c71+odȑgۃ#a1FY_OOש斊<9RJJY*3\hCl-*	;¿\mCآ5U0/lIJ4m킘46Z-o%긇{_RH2pgdzHW+{+7nL]V<TwbiM
ha0gfbjEVWV@+ݫU#7  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  <Notes>
<UUID>9c128e4c-85f0-490f-a6a5-155c96dcc7bf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBabeD puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="23:0:0">2014.4.3</Created>
<LastModified UTC="23:0:0">2014.4.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="2143-2472" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5772-5876" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="2082..2090" colors="white"/></TopStrand><BottomStrand><ColorRange range="2082..2090" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="1362..2087" colors="red"/></TopStrand><BottomStrand><ColorRange range="1362..2087" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="replace" val1="1362" val2="1367"/><Node name="33769.dna" type="DNA" seqLen="5179" ID="0" circular="1" useCustomMapLabel="1" customMapLabel="pBabeD puro" resurrectable="1" operation="invalid"><Features><Feature name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4982" detectionMode="exactProteinMatch"><Segment range="4191-4982" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="4983-5051" color="#ccffcc" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0"><Segment range="1762-2361" color="#ccffcc" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature name="LTR" type="LTR" allowSegmentOverlaps="0"><Segment range="2460-3053" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3425-4013" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="LTR" type="LTR" allowSegmentOverlaps="0"><Segment range="8-477" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;long terminal repeat from Moloney murine leukemia virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="942-1358" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="1423-1752" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5052-5156" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1362..1367" colors="white"/></TopStrand><BottomStrand><ColorRange range="1362..1367" colors="white"/></BottomStrand></StrandColors></HistoryColors></Node></Node></Node></Node></Node></HistoryTree>
  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="13"><Feature recentID="1" name="LC3" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2815-3192" color="#a6acb3" type="standard"/></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="6846-6950" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3397-3532" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="MMLV Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="542-741" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of Moloney murine leukemia virus (MMLV)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3217-3546" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="942-1358" color="#993366" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated MMLV&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; 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from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="3" name="AmpR" directionality="2" translationMW="31544.14" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6776" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5985-6776" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6777-6845" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="mCherry" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1363-2082" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3192" maxFusedRunOn="3192"><Segment range="2083-2085" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2086-2088" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2089-2799" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature></Features>
  B<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pBabe Rev" sequence="CCACACCTGGTTGCTGACTAATTGAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3282-3307" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3282-3307" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite location="3354-3372" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3354-3372" bases="TGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3282-3307" boundStrand="1" annealedBases="CCACACCTGGTTGCTGACTAATTGAG" meltingTemperature="62"><Component hybridizedRange="3282-3307" bases="CCACACCTGGTTGCTGACTAATTGAG"/></BindingSite><BindingSite simplified="1" location="3354-3372" boundStrand="1" annealedBases="TGGTTGCTGACTAATTGAG" meltingTemperature="50"><Component bases="CCACACC"/><Component hybridizedRange="3354-3372" bases="TGGTTGCTGACTAATTGAG"/></BindingSite></Primer><Primer recentID="1" name="pBabe Fwd" sequence="CCTCCTCTTCTTCCATCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite><BindingSite simplified="1" location="1256-1273" boundStrand="0" annealedBases="CCTCCTCTTCTCCATCC" meltingTemperature="46"><Component hybridizedRange="1256-1265" bases="CCTCCTCTTC"/><Component bases="T"/><Component hybridizedRange="1267-1273" bases="TCCATCC"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>43936b29-fe54-4c85-85f3-83e94ce0271a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBabeD puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="23:0:0">2014.4.3</Created>
<LastModified UTC="12:16:32">2021.5.6</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                   HF  d               E                                                                                                                                                                                          j<?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="AarI AccI AfeI AflIII ApaI AvrII BamHI BclI BfuAI BglII BmtI BsaAI BsiWI BspMI BspQI BstXI DraIII EcoRI FspI HindIII NaeI NgoMIV NheI NotI PciI PflMI PmeI PspOMI PspXI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgrAI SpeI SspI XcmI XmnI"/></CustomEnzymeSets>
