Services | Custom cDNA Clone Production

st step to designing your clone is a discussion with a member of our highly experienced staff.  We can work with you to identify:

• The optimal plasmid backbone (see further below as well) for end-use expression systems (bacterial, mammalian, baculovirus/insect cells, yeast systems)
• The best source for your material for your gene of interest
• Any mutagenesis requirements for your gene of interest
• Any combination of tags that may be required for end-use.

Depending on the planned end use of the cDNA construct you have generated, we would be happy to have ensure that purification of the DNA meets those goals. Considerations of quantity and concentration will depend largely on the type of backbone and size of the construct. We can discuss all these matters with you at the outset of the project.

Information to be added

Upon completion of construction, all clones are checked by digestion and full sequencing of the expressed open reading frame.

As each investigator's needs for cDNA construct generation are different, pricing will vary depending on all the variables discussed above including:

• Size of backbone and insert
• Mutagenesis requirements
• Addition of tags
• Purification

Please contact us to discuss the requirements of your project in further detail!

Given that the nature of each customer's project is unique, turnaround times will necessarily vary from clone to clone.  We will endeavour to provide each customer with a realistic timeline for their clone however, at the initial discussion phase.