cDNA Clone CDK5RAP3

• Plasmid: pEX 8His mMBP C3 CDK5RAP3
• Parent Plasmid: pEX mMBP
• Expressed: 8His mMBP C3 CDK5RAP3
• Genbank: NP_788276.1
• Tag: MBP

Sequence of Insert

catATGGGCCACCACCACCACCACCACCACCACAAGACCGAGGAGGGCAAGCTCGTCATCTGGATCAACGGCGACAAGGGCTACAACGGCCTCGCCGAGGTCGGCAAGAAGTTCGAGAAGGACACCGGCATCAAGGTCACCGTCGAGCACCCCGACAAGCTCGAGGAGAAGTTCCCCCAGGTCGCCGCCACCGGCGACGGCCCCGACATCATCTTCTGGGCCCACGACCGCTTCGGCGGCTACGCCCAGTCCGGCCTCCTCGCCGAGATCACCCCCGCCGCCGCCTTCCAGGACAAGCTCTACCCCTTCACCTGGGACGCCGTCCGCTACAACGGCAAGCTCATCGCCTACCCCATCGCCGTCGAGGCCCTCTCCCTCATCTACAACAAGGACCTCCTCCCCAACCCCCCCAAGACCTGGGAGGAGATCCCCGCCCTCGACAAGGAGCTCAAGGCCAAGGGCAAGTCCGCCCTCATGTTCAACCTCCAGGAGCCCTACTTCACCTGGCCCCTCATCGCCGCCGACGGCGGCTACGCCTTCAAGTACGCCGCCGGCAAGTACGACATCAAGGACGTCGGCGTCGACAACGCCGGCGCCAAGGCCGGCCTCACCTTCCTCGTCGACCTCATCAAGAACAAGCACATGAACGCCGACACCGACTACTCCATCGCCGAGCACGCCTTCAACCACGGCGAGACCGCCATGACCATCAACGGCCCCTGGGCCTGGTCCAACATCGACACCTCCGCCGTCAACTACGGCGTCACCGTCCTCCCCACCTTCAAGGGCCAGCCCTCCAAGCCCTTCGTCGGCGTCCTCTCCGCCGGCATCAACGCCGCCTCCCCCAACAAGGAGCTCGCCAAGGAGTTCCTCGAGAACTACCTCCTCACCGACGAGGGCCTCGAGGCCGTCAACAAGGACAAGCCCCTCGGCGCCGTCGCCCTCAAGTCCTACGAGGAGGAGCTCGTCAAGGACCCCCGCGTCGCCGCCACCATGGAGAACGCCCAGAAGGGCGAGATCATGCCCAACATCCCCCAGATGTCCGCCTTCTGGTACGCCGTCCGCACCGCCGTCATCAACGCCGCCTCCGGCCGCCAGACCGTCGACGCCGCCCTCGCCGCCGCCCAGACCAACGCCGCCGCCCTGGAAGTTCTGTTCCAGGGGCCCCTGGTCGACATGGAGGACCATCAGCACGTGCCCATCGACATCCAGACCAGCAAGCTGCTCGATTGGCTGGTGGACAGAAGGCACTGCAGCCTGAAATGGCAGAGTCTGGTGCTGACGATCCGCGAGAAGATCAATGCTGCCATCCAGGACATGCCAGAGAGCGAAGAGATCGCCCAGCTGCTGTCTGGGTCCTACATTCACTACTTTCACTGCCTAAGAATCCTGGACCTTCTCAAAGGCACAGAGGCCTCCACGAAGAATATTTTTGGCCGATACTCTTCACAGCGGATGAAGGATTGGCAGGAGATTATAGCTCTGTATGAGAAGGACAACACCTACTTAGTGGAACTCTCTAGCCTCCTGGTTCGGAATGTCAACTATGAGATCCCCTCACTGAAGAAGCAGATTGCCAAGTGCCAGCAGCTGCAGCAAGAATACAGCCGCAAGGAGGAGGAGTGCCAGGCAGGGGCTGCCGAGATGCGGGAGCAGTTCTACCACTCCTGCAAGCAGTATGGCATCACGGGCGAAAATGTCCGAGGAGAACTGCTGGCCCTGGTGAAGGACCTGCCGAGTCAGCTGGCTGAGATTGGGGCAGCGGCTCAGCAGTCCCTGGGGGAAGCCATTGACGTGTACCAGGCGTCTGTGGGGTTTGTGTGTGAGAGCCCCACAGAGCAGGTGTTGCCAATGCTGCGGTTCGTGCAGAAGCGGGGAAACTCAACGGTGTACGAGTGGAGGACAGGGACAGAGCCCTCTGTGGTGGAACGACCCCACCTCGAGGAGCTTCCTGAGCAGGTGGCAGAAGATGCGATTGACTGGGGCGACTTTGGGGTAGAGGCAGTGTCTGAGGGGACTGACTCTGGCATCTCTGCCGAGGCTGCTGGAATCGACTGGGGCATCTTCCCGGAATCAGATTCAAAGGATCCTGGAGGTGATGGGATAGACTGGGGAGACGATGCTGTTGCTTTGCAGATCACAGTGCTGGAAGCAGGAACCCAGGCTCCAGAAGGTGTTGCCAGGGGCCCAGATGCCCTGACACTGCTTGAATACACTGAGACCCGGAATCAGTTCCTTGATGAGCTCATGGAGCTTGAGATCTTCTTAGCCCAGAGAGCAGTGGAGTTGAGTGAGGAGGCAGATGTCCTGTCTGTGAGCCAGTTCCAGCTGGCTCCAGCCATCCTGCAGGGCCAGACCAAAGAGAAGATGGTTACCATGGTGTCAGTGCTGGAGGATCTGATTGGCAAGCTTACCAGTCTTCAGCTGCAACACCTGTTTATGATCCTGGCCTCACCAAGGTATGTGGACCGAGTGACTGAATTCCTCCAGCAAAAGCTGAAGCAGTCCCAGCTGCTGGCTTTGAAGAAAGAGCTGATGGTGCAGAAGCAGCAGGAGGCACTTGAGGAGCAGGCGGCTCTGGAGCCTAAGCTGGACCTGCTACTGGAGAAGACCAAGGAGCTGCAGAAGCTGATTGAAGCTGACATCTCCAAGAGGTACAGCGGGCGCCCTGTGAACCTGATGGGAACCTCTCTGTGAggtacc

Amino Acid Sequence

MGHHHHHHHHKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAALEVLFQGPLVDMEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVLTIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLRILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYEKDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQQEYSRKEEECQAGAAEMREQFYHSCKQYGITGENVRGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQASVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGTEPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEGTDSGISAEAAGIDWGIFPESDSKDPGGDGIDWGDDAVALQITVLEAGTQAPEGVARGPDALTLLEYTETRNQFLDELMELEIFLAQRAVELSEEADVLSVSQFQLAPAILQGQTKEKMVTMVSVLEDLIGKLTSLQLQHLFMILASPRYVDRVTEFLQQKLKQSQLLALKKELMVQKQQEALEEQAALEPKLDLLLEKTKELQKLIEADISKRYSGRPVNLMGTSL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pEX 8His mMBP C3 CDK5RAP362.02 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU59674
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: backbone 59582 pEX mMBP c-GFP, precursor to this plasmid is 59629
• Low Temp: No