Full Name
MSK1 (384 - 402)
Long Name Textual
mitogen and stress-activated protein kinase-1
Synonyms
RPS6KA5
Immuno Sequence
FKRNAAVIDPLQFHMGVER [residues 384 - 402 of human]
Mono/Poly clonal
Polyclonal
Gel Image
2 mg of cellular extract from Hek 293 cells were pre-cleared with 50 μl of protein G sepharose for 30 minutes at 4 °C before the supernatant was incubated with 5 μg of anti- MSK1 (384 – 402) and 50 μl of protein G sepharose for 1 hour at 4 °C. The supernatant was removed and the pellets washed with lysis buffer before being subjected to SDS/PAGE and transferred to nitrocellulose membrane. The membrane was then immunoblotted with anti- MSK1 (384 – 402) at a concentration of 1 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Sheep No
S693B
Unit Source
Aliquot
0.1mg
Price
£150.00