Full Name
PINK (125 - 539)
Long Name Textual
PTEN induced putative kinase 1
Immuno Sequence
PINK (125 - 539) MBP cleaved [DU 13946]
Mono/Poly clonal
Polyclonal
Gel Image
HEK293 cells were either untransfected (lane one) or transfected with full length (1-581) PINK1-myc cDNA (lane two). 25μg of protein was subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 (125 – 539) at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.
PINK1 was immunoprecipitated from 1mg HEK 293 cellular extracts over-expressing humanwildtypePINK1. Immunoprecipitateswereperformedusing5μgofpre-immune serum covalently coupled to protein G sepharose (lane 1)
Sheep No
S460C
Unit Source
Aliquot
0.1mg
Price
£150.00