Full Name
PFKFB3 phospho Ser 461
Long Name Textual
6-phosphofructo-2-kinase / fructose-2,6-biohosphatase 3
Synonyms
PFKFB3
Immuno Sequence
CPLMRRNS*VTPLAS [residues 455 - 467 of human]
Mono/Poly clonal
Polyclonal
Gel Image
In (a) the indicated ammounts of phospho-Ser 461 peptide (P) and non-phosphopeptide (N) [residues 455-467 of human ]were spotted on nitrocellulose. The antibody is phospho-specific in peptide dot blots when 10 x non-phosphopeptide is included to eliminate background binding to the unphosphorylated peptide. Thus far, only used in Western blots of recombinant, transiently expressed GFP-tagged proteins in HEK293 cells where it can recognise GFP-PFKFB3 pSer 461 in 20 μg lysate (b). In (b), transiently expressed GFP-PFKFB3 was isolated from HEK293 cells treated or not with the Ser/Thr phosphatase inhibitor Calyculin A. The GFP immunoprecipitates were dephosphorylation with lambda phosphatase in vitro where indicated in the absence and presence of EDTA as a phosphatase inhibitor then probed with pSer 461 using anti-GFP as loading control.
Sheep No
S090D
Unit Source
Aliquot
0.1mg
Price
£125.00