Full Name
CRTC3 (142 - 151)
Long Name Textual
CREB regulated transcription coactivator 3
Synonyms
CRTC3
Immuno Sequence
CWKEEKHPGFR [residues 142 - 151 of human]
Mono/Poly clonal
Polyclonal
Gel Image
Flag-CRTC3 was expressed in HEK293 cells. CRTC3 was immunoprecipitated from 0.25 mg cell extract protein with anti-FLAG or different antibodies against CRTC3 (2.5 ug). Immunoprecipitation of overexpressed CRTC3 was monitored by immunoblotting using anti-FLAG. CRTC3 antibodies raised in sheep S276D anti-CRTC3 (3 – 13), S277D anti-CRTC3 (142 – 155), and S279D anti-CRTC (pan isoform), but not S278D anti-CRTC3 (598 – 608), immunoprecipitate overexpressed CRTC3.
Cell extract protein from RAW264.7 cells (0.75 mg) was incubated with increasing amounts of S277D anti-CRTC3 (142 – 151) (bleed 2) and proteins precipitated using protein G-Sepharose. Immunoprecipitated CRCT3 was detected by immunoblotting using anti-CRTC3 (Abcam cat. Ab91654). Depletion of CRTC3 from extracts was also measured by blotting cell extracts after immunoprecipitation. Protein amounts on gel were verified by immunoblotting for tubulin. The antibody against CRTC3 S277D can immunoprecipitate endogenous CRTC3 and can completely deplete CRTC3 from the extract.
PDF Datasheet
CRTC3132.98 KB
Sheep No
S277D
Unit Source
Aliquot
0.1mg
Price
£150.00