cDNA Clone ZNRF2 (mouse)

• Plasmid: pGEX6P-3-(mouse) ZNRF2
• Parent Plasmid: pGEX6P3
• Genbank: NP_954594.1 GI:40255287
• Species: Mouse

Sequence of Insert

GGATCCATGGGCGCAAAACAGAGCGGACCAGCAGCTAATGGACGCACACG
CGCATATAGCGGCAGCGATCTGCCGAGCGGCACAGGTTCCGGAGGAGGCG
GAGCAGATGGAGCTCGCGCAGCTCGCTTTGCTGCTCCAGTGAGCGGCGCA
CAGCAGCCGTCCGCAAGCGCTGGAGCAGCCGCTGCTGCAGCCGCAGCTGC
TAGCGCACCAGCTGCACCACGCTCCCGCAGCCTGGGCGGAGCAGTGGGAA
GCGCTTCCGGAGGCCGCGCAGCCCAGTCCGCATTCAGCATTCCGTCCGCA
GGCGGAGGCGGAGGACCATATGGCAGCCAGGATAGCGTGCACAGCAGCCC
AGAAGATAGCGTGGGAGCTCGCGATCGCGATCGCCCGGCTGGCGGAGGCC
CAGGAGGACCACGCCTGGTGATTGGCAGCCTGCCGGCACACCTGAGCCCG
CATCTGTTTGGCGGATTCAAATGCCCAGTGTGCAGCAAATTTGTGCCGAG
CGATGAAATGGATCTGCACCTGGTGATGTGCCTGACCAAACCACGCATCA
CCTACAACGAAGATGTGCTGAGCAAAGATACCGGAGAGTGCGCCATCTGC
CTGGAAGAGCTGCAGCAGGGCGATACCATTGCCCGCCTGCCGTGCCTGTG
CATCTATCACAAAGGATGCATCGATGAATGGTTTGAAGTGAACCGCAGCT
GCCCAGAACATCCAAGCGATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGAKQSGPAANGRTRAYSG
SDLPSGTGSGGGGADGARAARFAAPVSGAQQPSASAGAAAAAAAAASAPA
APRSRSLGGAVGSASGGRAAQSAFSIPSAGGGGGPYGSQDSVHSSPEDSV
GARDRDRPAGGGPGGPRLVIGSLPAHLSPHLFGGFKCPVCSKFVPSDEMD
LHLVMCLTKPRITYNEDVLSKDTGECAICLEELQQGDTIARLPCLCIYHK
GCIDEWFEVNRSCPEHPSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36367
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DNA sequence optimised to reduce GC-content.; Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 50526 Daltons; ; All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.