cDNA Clone DCUN1D1

• Plasmid: pTRE-Tight-SV5-DCUN1D1 D211A,A235R,D241A
• Parent Plasmid: pTRE-Tight
• Expressed: SV5-DCUN1D1 D211A,A235R,D241A
• Genbank: NM_020640*
• Tag: SV5
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACCATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTTTATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTCAAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCTGAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTTAGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTGGAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCCAGCATTAGTGTGTTGATTATTGCGTGGAAGTTCAGAGCAGCAACACAGTGCGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTGACAGCATA
GAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTGAAAGAACC
AGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGCAAAGAATC
CAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACTGGAACTTA
GTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAATTTTTGTT
GGAACATCATAAACGATCAATACCAAAAGCCACTTGGAATCTTCTTTTAG
ACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATGAAGAAGGA
AGATGGCCTGTTCTTATTGCTGACTTTGTGGAATTTGCACGCCCTCAAAT
TGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MGKPIPNPLLGLDSTMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDW
KLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDG
IQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIE
KLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLV
LNGRFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGR
WPVLIADFVEFARPQIAGTKSTTV*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20581
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries g318a mutation (silent Ala106) and the following substitutions:; a632c (confers D211A), g703a/c704g (confers A235R) and a722c (confers D241A).; Mammalian response plasmid for expression of SV5-DCUN1D1 D211A,A235R,D241A mutant (for use with Clontech Advanced Tet-ON system); ; Estimated MW of expressed protein : 31528 Daltons