cDNA Clone DCUN1D5

• Plasmid: pEBG6P-DCUN1D5
• Parent Plasmid: pEBG6P
• Expressed: GST-DCUN1D5
• Genbank: NM_032299
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGC
AGTAGCGGAAGACGGAGGCCTCAAAAAGTGTAAAATCTCCAGCTATTGCA
GATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTCAAGC
AAGAAGTGCCTGGCTTGGTTTTATGAATATGCAGGTCCTGATGAAGTTGT
AGGGCCAGAAGGAATGGAAAAATTTTGTGAAGACATTGGTGTTGAACCTG
AAAATATTATTATGTTAGTTTTAGCGTGGAAATTGGAGGCTGAAAGCATG
GGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACTTCATTACAGTG
TGACTGCACAGAAAAGTTACAAAACAAATTTGACTTTTTGCGCTCACAGT
TGAATGATATTTCGTCATTTAAGAATATCTACAGATATGCCTTTGATTTT
GCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCTAAATCTAT
GTTAGCTCTTCTGCTTGGGAGGACATGGCCACTGTTTTCAGTATTTTACC
AGTACCTGGAGCAATCAAAGTATCGTGTTATGAACAAAGATCAATGGTAC
AATGTATTAGAATTCAGCAGAACAGTCCATGCTGATCTTAGTAACTATGA
TGAAGATGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAA
AAGTCCGTCAGACATCATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPVKKKRKSPGVA
AAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWFYEYAGPDE
VVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL
QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFARDKDQRSLDIDTAK
SMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHADLSN
YDEDGAWPVLLDEFVEWQKVRQTS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20501
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express DCUN1D5 with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 54943 Daltons