cDNA Clone IRAK2 216-504

• Plasmid: pET156P1-TEV-IRAK 216-504
• Parent Plasmid: pET15b 6His 3C
• Expressed: Bacterial
• Tag: 6His

Sequence of Insert

ATCAGCCAGGGGACCTTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGAGAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATTTGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAGTTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGTCAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTCTGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAATGTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCTGTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCGTATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGCTGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGCCCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCGCTCTGCTCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAGAAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTGTGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAAGAGCGGCTCCGATGA

Amino Acid Sequence

MGSSHHHHHHNSGLQLLFQGPGSENLYFQISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSIERFFQAELQICLRCCHPNVLPVLGFCAARQFHSFIYPYMANGSLQDRLQGQGGSDPLPWPQRVSICSGLLCAVEYLHGLEIIHSNVKSSNVLLDQNLTPKLAHPMAHLCPVNKRSKYTMMKTHLLRTSAAYLPEDFIRVGQLTKRVDIFSCGIVLAEVLTGIPAMDNNRSPVYLKDLLLSDIPSSTASLCSRKTGVENVMAKEICQKYLEKGAGRLPEDCAEALATAACLCLRRRNTSLQEVCGSVAAVEERLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Previously it was cloned in GST-TEV tagged. BUt when I cleave the tag all protein precipitated. Can you please make Histev site intead of GST tag. ; Crystallisation purpose I can use it without cleave the tag, GST is too big.