cDNA Clone IL1a (mouse)

• Synonyms: IL1 alpha
• Plasmid: pGEX6P1 IL1a (mouse) 115-270
• Parent Plasmid: pGEX6P1
• Expressed: GST IL1a (mouse) 115-270
• Genbank: P01582
• Tag: GST

Sequence of Insert

GGATCCTCAGCACCTTACACCTACCAGAGTGATTTGAGATACAAACTGATGAAGCTCGTCAGGCAGAAGTTTGTCATGAATGATTCCCTCAACCAAACTATATATCAGGATGTGGACAAACACTATCTCAGCACCACTTGGTTAAATGACCTGCAACAGGAAGTAAAATTTGACATGTATGCCTACTCGTCGGGAGGAGATGACTCTAAATATCCTGTTACTCTAAAAATCTCAGATTCACAACTGTTCGTGAGCGCTCAAGGAGAAGACCAGCCCGTGTTGCTGAAGGAGTTGCCAGAAACACCAAAACTCATCACAGGTAGTGAGACCGACCTCATTTTCTTCTGGAAAAGTATCAACTCTAAGAACTACTTCACATCAGCTGCTTATCCAGAGCTGTTTATTGCCACCAAAGAACAAAGTCGGGTGCACCTGGCACGGGGACTGCCCTCTATGACAGACTTCCAGATATCATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSAPYTYQSDLRYKLMKLVR
QKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKY
PVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINS
KNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46302
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you clone residues 115-270 of mouse IL-1 alpha into a pGEX vector for bacterial expression. Thanks,; Sam