cDNA Clone HHARI-HOIP chimera

• Plasmid: pGEX TEV HHARI (D183-S230) - HOIP (C744-K1072)
• Parent Plasmid: pGEX TEV
• Expressed: GST-TEV-HHARI (D183-S230) - HOIP (C744-K1072)
• Genbank: NM_017999.4 (HOIP) and NP_005735 (HHARI)
• Tag: GST
• Species: Human

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGEFDMPCQICYLNYPNSYFTGLEC
GHKFCMQCWSEYLTTKIMEEGMGQTISCPACGRPDLTDDTQLLSYFSTLD
IQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEAT
CPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQE
NGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNC
RVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPG
GGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLD
PATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQS
IPRRRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24314
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (DU20757) parental. Two rounds of insertion mutagenesis with NW2676/77 and NW2678/79 to replace HOIP 697-743 (QEC....TDMV) in DU22629 with HHARI 183-230 (DMPC...QTIS). mutations in precursor corrected with NW2730/31