cDNA Clone GST-HALO-ASB5

• Synonyms: ankyrin repeat and SOCS box containing 5
• Plasmid: pGEX6P HALO Thr ASB-5
• Parent Plasmid: pGEX6P Halo Thr
• Expressed: GSTHALO-ASB-5
• Genbank: NM_080874.3 Q8WWX0
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTCGGTGTTAGAAGAAAATCGGCCGTTTGCTCAACAATTATC
CAATGTCTACTTTACAATACTTTCGCTGTTCTGTTTTAAGCTTTTTGTGA
AAATCAGCCTTGCCATCCTCAGTCATTTCTACATAGTGAAAGGCAACCGC
AAGGAAGCGGCAAGGATAGCAGCTGAATTTTATGGAGTAACCCAAGGACA
AGGTTCCTGGGCAGATCGATCACCACTACATGAAGCAGCAAGTCAAGGTC
GCCTTCTTGCTCTGAGAACATTATTATCACAGGGTTATAATGTAAATGCA
GTAACCTTAGACCATGTCACCCCATTGCACGAAGCCTGCCTTGGAGATCA
CGTGGCATGTGCCAGAACTCTGCTGGAAGCAGGAGCTAATGTAAATGCAA
TCACGATAGATGGCGTGACTCCGTTATTCAACGCATGCTCCCAAGGCAGT
CCAAGCTGTGCAGAGCTGCTTCTGGAGTATGGTGCCAAAGCCCAGCTGGA
GTCATGTCTTCCCTCCCCAACGCATGAGGCCGCCAGTAAAGGTCACCATG
AATGTCTTGACATCCTGATATCCTGGGGCATAGATGTTGACCAAGAAATT
CCTCATTTGGGAACTCCTCTCTATGTAGCTTGTATGTCACAGCAATTCCA
TTGCATCTGGAAGCTTCTTTATGCTGGTGCTGACGTACAGAAAGGCAAAT
ATTGGGATACTCCATTACATGCTGCTGCTCAACAATCCAGCACAGAAATT
GTAAACTTACTGCTAGAATTTGGAGCAGATATCAATGCCAAAAATACAGA
GCTTCTGCGACCTATAGATGTAGCTACGTCTAGCAGTATGGTGGAAAGAA
TATTGCTTCAACATGAAGCTACCCCAAGCTCTCTTTACCAACTTTGCCGA
CTCTGTATCCGAAGCTACATAGGAAAACCAAGATTGCACCTTATCCCACA
ACTCCAGCTGCCAACGTTACTGAAGAATTTCTTACAGTATCGATAAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMSVLEENRPFAQQL
SNVYFTILSLFCFKLFVKISLAILSHFYIVKGNRKEAARIAAEFYGVTQG
QGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTLDHVTPLHEACLGD
HVACARTLLEAGANVNAITIDGVTPLFNACSQGSPSCAELLLEYGAKAQL
ESCLPSPTHEAASKGHHECLDILISWGIDVDQEIPHLGTPLYVACMSQQF
HCIWKLLYAGADVQKGKYWDTPLHAAAQQSSTEIVNLLLEFGADINAKNT
ELLRPIDVATSSSMVERILLQHEATPSSLYQLCRLCIRSYIGKPRLHLIP
QLQLPTLLKNFLQYR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24339
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: two silent mutations compared to NCBI ref at Pro169 (CCA instead of CCC) and Arg281 (AGA instead of AGG). Parental 24192