cDNA Clone FLAG-TNF alpha

• Plasmid: pCMV5 FLAG TNF alpha 77-234
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TNF alpha 77-234
• Genbank: AAA61198.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGTCAGATCATCTTCTCGAACCCCGAGTGACAAGCCTGTAGCCCA TGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTG GTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAA GGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCC GCATCGCCGTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAG AGCCCCTGCCAGAGGGAGACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTA TGAGCCCATCTATCTGGGAGGGGTCTTCCAGCTGGAGAAGGGTGACCGAC TCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTTGCCGAGTCTGGG CAGGTCTACTTTGGGATCATTGCCCTGTGAGGATCC

Amino Acid Sequence

MDYKDDDDKGSVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQT
KVYLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPNYLDFAESGQVYFGIIAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51044
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: We would like to express a N-term. FLAG-tagged version based on DU8654 (TNF alpha)