cDNA Clone FANCI (Gallus gallus)

• Plasmid: pGEX6P1 Halo chicken FancI
• Parent Plasmid: pGEX6P1 Halo
• Expressed: GST-3C-HALO-FANCI
• Genbank: NM_001114851.1 GI:168803993 with killed internal BamHI
• Tag: GST
• Tag: HALO
• Species: Chicken

Sequence of Insert

GGATCCATGGCGCAGCGCATCCTGCAGCTGGCGGCCGAGGGGAGCCCCGAGCGGCTCCAGGAGGCTCTTCAGGGCCTGACGGAGGGCGAGCTGGGTGACATGGTGACGAGGCAGGCCCTGAGGGGCAGGGAGACGGCAGCGCTGCTTAAGGGGATCTTCAAAGGCTCCCCATGTTCCCAGCAAAGCGGTGTTCTCAGGAGGCTGCAGGTTTACAAGCACTGTGTCTCACTGGTGGAATCAGGAGATCTGCATGTGGGCAAAGTGTCCGAAATCATAGGGCTGCTGATGCTGGAGGCTCGCCAGCTGCCAGGCCATGCGTTGGCTGAACTTGCCACCTTGTTTGTTGAAGTTATTAAGAGAGGCAGCCTGTCTAATGGGAAATCCTTGGAGTTGTTTTCCACCGTTCTTACTGCGTTGTCCAACTCTAAGGAGAGCCTGGCTTATGGGAAAGGTGAACTGAACGGGGAAGAATTTAAGAAACAGCTGATAAATACCCTCTGCTCCAGCAAGTGGGACCCTCAGTGTGTAATACATCTTGCCAACATGTTCAGGGATATTCCACTATCAGGAGAGGAGCTGCAGTTTGTGGTGGAAAAGGTCCTTAGGATGTTCTCCAAATTAGACCTGCAGGAAATACCCCCCCTGGTCTATCAGCTGCTGCTGCTTTCTGCAAAGGGCAGTAAGAAAACTGTTTTAGAAGGAATCATCAGTTTTTTCAATCAGTTGGACAAGAGACAAAAGGAAGAGCAGAGGGTTCCTCAATCAGCAGACCTTGAGGTAGCCACAGTGCCCCTAGACCAGCTCCGCCACGTGGAAGGTACCGTTATCCTCCACATTGTTTCTGCTATCAACCTGGATCAGGACATTGGTGAGGAGCTCATCAAACATCTGAAGACTGAGCAACAGAAGGACCCTGGTAAAGCCCTGTGTCCCTTCAGTGTGTCCCTTTTGCTTTCGACTGCAGTGAAACACAGACTGCAAGAGCAGATATTTGATTTCTTGAAAACATCAATCACGAGAAGCTGCAAGGACCTGCAGATCCTACAGGCCTCCAAGTTTCTGCAGGATTTGTGTCCTCAGCAGTATGATGTAACAGCTGTAATTCTGGAAGTGGTGAAAAATAGTGCTTTTGGCTGGGACCATGTTACCCAGGGACTGGTAGATCTTGGCTTCAGCCTAATGGAATCGTATGAACCAAAAAAGTCCTTTGGAGGGAAAGCTGCTGAGACCAATTTGGGCCTTTCAAAAATGCCAGCACAGCAGGCTTGCAAACTGGGAGCGAGTATCCTGCTGGAAACCTTTAAGGTCCATGAGCCCATCAGAAGTGATATTCTTGAGCAAGTCCTGAACAGGGTCCTCACAAAAGCAGCATCTCCTGTCAGCCACTTCATAGACTTGCTGTCCAATATTGTTGTGTCTGCTCCTCTTGTGCTTCAGAACTCATCCTCCAGAGTCACAGAGACCTTTGACAATTTGTCCTTTCTGCCCATCGACACAGTGCAAGGGCTCCTCCGGGCAGTACAGCCCCTGCTCAAAGTCAGCATGTCGGTGAGGGACTCCCTGATACTTGTTCTCCAGAAAGCTATCTTTTCCAGGCAGCTCGATGCTCGTAAAGCTGCAGTTGCTGGCTTTTTGCTTCTGTTAAGAAATTTTAAGATTCTGGGCAGCTTGACTTCTTCCCAGTGCAGCCAGGCCATTGGTGCCACTCAGGTCCAGGCAGATGTTCATGCCTGCTATAATTCTGCAGCTAATGAGGCCTTCTGCCTTGAAATCCTGGGCAGCCTGAGGCGATGTCTGAGCCAGCAAGCAGATGTCCGACTCATGTTATATGAGGGTTTTTATGATGTCCTACGCAGGAACTCCCAGCTGGCCAGCTCTATAATGGAAACACTTTTGTCCCAGATAAAGCAATACTACTTGCCCCAGCAAGATCTCCTGCCTCCACTGAAACTTGAGGGATGTATAATGGCTCAAGGAGATCAAATCTTTTTGCAAGAACCACTGGCCCATCTGCTCTGCTGCATCCAGCACTGTCTAGCCTGGTATAAGAGCACTGTGCATCTATGCAAAGGAGCTGAAGATGAGGAGGAGGAGGAGGATGTGGGATTTGAGCAAAACTTTGAAGAGATGTTAGAATCTGTCACACGACGGATGATCAAGAGTGAGCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCCATCTTCTGGTGTTGGTGTAAAGAATAACATCTATGCCATCCAGGTGATGGGAATTTGCGAGGTCTTGATTGAGTACAATTTCAAAATAGGGAATTTCAGTAAGAACAAATTTGAAGATGTCCTAGGCCTGTTTACATGTTACAACAAGCTCTCAGAAATCTTGAAGGAGAAAGCTGGAAAGAATAAATCTACGTTGGGCAACAGAATTGCACGGAGCTTCCTCTCTATGGGTTTTGTGTCTACGCTGCTCACAGCTCTGTTCAGGGACAATGCTCAAAGCCACGAGGAGAGCCTGGCAGTGCTGCGCTCCAGCACGGAGTTCATGCGCTACGCTGTCAGTGTGGCACTACAGAAGGTGCAGCAGCTGGAGGAGATGGGACAAACCGACGGCCCGGATGGACAAAACCCTGAGAAGATGTTTCAGAACCTCTGCAAAATCACACGGGTTCTGCTTTGGAGATACACTTCAATTCCCACTGCTGTTGAAGAGTCAGGGAAGAAGAAGGGCAAAAGCATTTCCCTGCTGTGCTTGGAAGGTTTGCTGCGGATCTTCAACACCATGCAGCAGCTGTACGCTGCTAGGATTCCCCAGTTTCTACAGGCCCTGGATATTACTGATGGTGACGCAGAAGAAGCGGATATTAATGTCACAGAGAAAGCTGCCTTCCAGATCCGACAGTTTCAGAGGTCTCTGGTGAACCAGCTCAGTAGTGCTGAAGATGACTTCAACTCCAAGGAAACACAGTTACTCATCACAATCCTCTCCACTTTGTCCAAACTCCTGGACCCAGGCTCTCAGCAGTTCCTTCAGTTCCTTACTTGGACAGTCAAAATTTGCAAAGAAAATGCTTTAGAGGATCTCTCTTGCTGTAAGGGTTTGCTGACTTTGCTTTTTAGTCTCCATGTTCTGTACAAGAGTCCTGTCAGTCTGCTGCGTGAACTTGCTCAAGATATCCATGCTTGCCTGGGAGACATAGATCAGGATGTAGAAATAGAGAGTCGATCCCATTTTGCCATAGTGAATGTGAAGACTGCAGCCCCTACTGTCTGCCTGCTGGTTCTGGGGCAGGCAGATAAGGTCCTTGAAGAGGTGGATTGGCTTATCAAGAGGCTTACCATCCTGGGGTCAGACACATCAGAAGACTCGACTCAGGCATCAAACCAAACCCAGGCTCTGGAGAAAGGTGTGATTCTGCAGTTGGGAACTCTGCTGACAGTTTTTCATGAGCTGGTGCAGACAGCACTTCCTGCAGGGAGCTGTGTAGACTCGCTGCTGAGAAGCCTCAGCAAGACGTATGCAATCCTCACCTCCCTCATCAAGCATTATATCCAAGCTTGCCGCAGCACCTCAAATACGGTTCCAGGGAGGCTTGAAAAGCTGGTGAAGCTCTCGGGTTCCCATTTGACCCCACAGTGTTACTCATTCATTACTTATGTACAGAACATCCATAGTGAGAGCTTAAGCTTTGCAGAAGAGAAGAAAAAGAAGAAGAAAGAAGACGAAACTGCTGTAGTCTCCACAGTCATGGCTAAGGTGCTTCGGGACACCAAGCCAATTCCAAACCTGATTTTTGCTATAGAGCAATACGAGAAGTTCCTTATCCATCTTTCGAAGAAATCAAAGGTGAACTTGATGCAATATATGAAACTAAGCACCTCCCGGGACTTCCGCATCAACGCATCCATGCTAGACAGTGTGCTGCAAGAGCAGAACACAGAGGATGCTGAAAATGAACCAGACAACAACCAGTCCGGCACAGCAGAGCAGCCAGATGAAAACCAGGAACCCCAAAAGAAAAGAAGGCGAAAAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGGSMAQRILQLAAEGSPERLQEALQGLTEGELGDMVTRQALRGRETAALLKGIFKGSPCSQQSGVLRRLQVYK
HCVSLVESGDLHVGKVSEIIGLLMLEARQLPGHALAELATLFVEVIKRGSLSNGKSLELFSTVLTALSNSKESLAYGKGELNGEEFKKQLINTLCSSKWD
PQCVIHLANMFRDIPLSGEELQFVVEKVLRMFSKLDLQEIPPLVYQLLLLSAKGSKKTVLEGIISFFNQLDKRQKEEQRVPQSADLEVATVPLDQLRHVE
GTVILHIVSAINLDQDIGEELIKHLKTEQQKDPGKALCPFSVSLLLSTAVKHRLQEQIFDFLKTSITRSCKDLQILQASKFLQDLCPQQYDVTAVILEVV
KNSAFGWDHVTQGLVDLGFSLMESYEPKKSFGGKAAETNLGLSKMPAQQACKLGASILLETFKVHEPIRSDILEQVLNRVLTKAASPVSHFIDLLSNIVV
SAPLVLQNSSSRVTETFDNLSFLPIDTVQGLLRAVQPLLKVSMSVRDSLILVLQKAIFSRQLDARKAAVAGFLLLLRNFKILGSLTSSQCSQAIGATQVQ
ADVHACYNSAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSIMETLLSQIKQYYLPQQDLLPPLKLEGCIMAQGDQIFLQEPLAHLL
CCIQHCLAWYKSTVHLCKGAEDEEEEEDVGFEQNFEEMLESVTRRMIKSELEDFELDKSADFSPSSGVGVKNNIYAIQVMGICEVLIEYNFKIGNFSKNK
FEDVLGLFTCYNKLSEILKEKAGKNKSTLGNRIARSFLSMGFVSTLLTALFRDNAQSHEESLAVLRSSTEFMRYAVSVALQKVQQLEEMGQTDGPDGQNP
EKMFQNLCKITRVLLWRYTSIPTAVEESGKKKGKSISLLCLEGLLRIFNTMQQLYAARIPQFLQALDITDGDAEEADINVTEKAAFQIRQFQRSLVNQLS
SAEDDFNSKETQLLITILSTLSKLLDPGSQQFLQFLTWTVKICKENALEDLSCCKGLLTLLFSLHVLYKSPVSLLRELAQDIHACLGDIDQDVEIESRSH
FAIVNVKTAAPTVCLLVLGQADKVLEEVDWLIKRLTILGSDTSEDSTQASNQTQALEKGVILQLGTLLTVFHELVQTALPAGSCVDSLLRSLSKTYAILT
SLIKHYIQACRSTSNTVPGRLEKLVKLSGSHLTPQCYSFITYVQNIHSESLSFAEEKKKKKKEDETAVVSTVMAKVLRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINASMLDSVLQEQNTEDAENEPDNNQSGTAEQPDENQEPQKKRRRKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Recommended propagation at 30 degrees.; Parental: pGEX6P-1-HALO (DU24133)