cDNA Clone FancI (Xenopus)

• Plasmid: pFastbac1 6His TEV V5 Xenopus FancI K524R (with mutations - see notes)
• Parent Plasmid: pFastbac1
• Expressed: 6His TEV V5 Xenopus FancI K524R (with mutations - see notes)
• Genbank: NM_001171211.1 GI:284005540*
• Tag: FLAG
• Species: Xenopus laevis

Amino Acid Sequence

MHHHHHHENLYFQGKPIPNPLLGLDSTMDQKILSLAAEEQNDGLQSYLQNLKETELSEIITKHAVKGKDCGALLRGVFKGSPCSHDVAVRRRLAVYRHCI
QLVESGDLQREVASEIMGLLMLEVHHFPGASLIELANLFADAVKAGTLSNGKSLDLFPTVLTALSSKESLAYGKGELTGEEFKKQLINSLCSSRWDPQSV
IFLTSMFRDVSLSAEELQFVVEKVLRMLSKLDLQEMPPLVYQLLLLSAKGSKKNIVEGIITIFNDLDQKQLVQQENSESLDLEDATIPQDQLHHVEGTII
LHIVFAIKLDQELGRELLKYLKAGQQGDSSKILCPFSAALLLSVSRIHRFQEQVFDFLKSTILRGYKDLQFHQSSKFLQDLVPQPVCVSSILLETVKNSV
YGWDHVTQGLVELGFILMDSFGPKAAFGNKVVELISVHSRTPSQQACQLGSRILLETFKVHEPIRSEILEQVLNRVITKAATPVTHFIDLLSDIVVSAPL
ILQNSSSKVTEAFDHLSFLPLTTVQGLLKAVQPLLKISMSMRDSLILVLRRAMFSSQIDARKSAVAGFLLLLRNFKVLGSLSSSQCSQAIGASQIQVDVH
MRYNAAANEAFCLEILGSLRRCLSQQADVRLMLYEGFYDVLRRNSQLASSVMQTLLSQLKRYYEPEPDLLPPLKLEGCITAQGDHIFLQEPLAHLLCCIH
HCLQWYKSSLQQHRNPDEDDDDDDQTGCQQDLNDIMESITRRMIKCDLEDFELDKSADFSLASGVGVKNNIYAVLVMGICEVLIEYNFIIANFSKSKFED
ILGLFKCYSKLSDILKEKAAKGRQPGNNKTARSLISMTFVSTLLTALFRDSTRSHEESLSILRANVDFMRYSVCVALQKIQQLEETGVTDGPDGQNSEKM
FRSLCEITRVLMWRYTSIPAAAENPGKKDKGKTISLLCLEGLLRVFNTVQQRYPSKIPQFLTALDALGDEDEEGSREINVTEKAAFQIKQFQRSLINQLS
GGEDDFNSKEALLLVSILSTLSRLLAPSSQQFVQMLSWTVKICKETNIEDVQFCKGIMNLLFSLHVQFKSPVSVLRELCQDIHGHLGDIDQDIEVEKQSH
FATVSLKTVAPTVTLLVLGQAAKVLEEVDWLIIKLKGLLGSEKLSTEDLTQTSNARVPIEKAIILQLGTLLTACHELVQTALPAGSCTDTLLKELAKMYT
ILTSVVKYYLQICSSHGGQISARLEKLVKLSGSHLTPQCYAFITYVQNIHSESLSLAAEKKKKKKEEALTATSAKILRDTKPIPNLIFAIEQYEKFLIHL
SKKSKVNLMQYMKLSTSRDFRINAATLDAALQEKGSEDEENEPDNEQAVTEEESQEPKKKRRRK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU50989
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Changes to NM_001171211.1 GI:284005540 in this clone: ; a1013g (D338G), g2342a (C781Y), c3245t (A1082V), t3731c (V1244A) and silent change c3753t.; Derived from clone provided by Mark Frost (Helen Walden group).