cDNA Clone IKKa

• Plasmid: pCMV5 FLAG2 IKKa
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG IKKa
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGTCTGTC TGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCGC CTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGAT TATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCTG AAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATAC TGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTG TGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTG GGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAAAACCT GAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAAT TGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTT TTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCT TACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATG TATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGC ATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAG ATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAATAGCCT TTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTGA ATTGCGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAG CCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACA CATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACCACCTG ATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATA AATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCC TCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGATA GCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCA TTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAG CAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGAAGCAG TGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTCAGGGA CAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAA AATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGG AGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAG ATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAAT GGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGG AGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGAAGAGC CCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAGCGTGC CATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACA GTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAG GACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTG TAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGTA ATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAAGGCAG AAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCTGCCCG GTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCAG CATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTG GTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTT GAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGAGGAAC AGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAATGAGCG GCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACD
VPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLC
TSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLIVEPMENWLQL
MLNCDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRG
CDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKA
KLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDH
SYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQ
TSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51461
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6th Dec 2017 NM_001278.4 update now calls residue 282 Trp (W)
• Low Temp: No