cDNA Clone FancI (mouse)

• Synonyms: FAAP43; PHF9; POG
• Plasmid: pFastbac1 6His TEV codon optimised mouse FancI M1-E1301 15TGS(MG) codon optimised FANCL tv2 Q109-E294
• Parent Plasmid: pFastbac1b
• Expressed: 6His TEV codon optimised mouse FancI M1-E1301 15TGS(MG) codon optimised FANCL tv2 Q109-E294
• Genbank: mouse FancI:codon optimised NM_145946.2 Human FANCL: codon optimised NM_018062.3 GI:167860136
• Tag: 6His

Sequence of Insert

CCATGGGCAGCAGCCATCACCATCACCATCACGAAAACCTGTATTTTCAGATGGACCTGAAGATCCTGTCCCTGGCTACCGACAAGACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACCTGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACCCTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGCTCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCGGCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTCGAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTTCGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAACTGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCTTGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGACACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCTCCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTGGCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCCACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTGTGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGCGAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGACGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTATCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCTGGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCTGCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACCTGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAGGGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCACCATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGACCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCCAAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAAGATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAACCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTGAACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCTGTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCAAAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTGCAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCATGCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGCTGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAACTTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGGTGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTAACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGCCAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCTGCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCCAGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAGTTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACCCCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGTCCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAAGAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTATGATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCTCCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATCATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTTCAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGAAGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATGACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCTGCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGTCCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACCCTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGACGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTGTGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGAAAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCAAAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAATTCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTCACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCTGCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCCTGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACCTCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGAATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCTTCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTGTCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAATCGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCACTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTCGACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTCTGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCCTGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTCTTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACACCCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGAAGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTCGAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTCCTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCGAAAAGGAAAAGGCTGCTGTCTCCACCACAATGGCTAAGGTCCTGCGCGAGACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTTCCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGAAGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATGGTGCTCCGCGAGGATGAGACTGGATCGACCGGTTCAACCGGATCCACCGGGAGCACAGGCTCTACGGGATCGACTGGATCGACCGGTTCAACGGGGAGCACTGGTTCAACAGGTTCCACCGGAAGCACAGGAAGTACAGGGTCCACTGGAAGTATGGGACAGTTTTATAGCAGCCTGATTGAAGAAATTGGCACCCTGGGCTGGGATAAACTGGTGTATGCGGATACCTGCTTTAGCACCATTAAACTGAAAGCGGAAGATGCGAGCGGCCGTGAACATCTGATTACCCTGAAACTGAAAGCCAAATATCCGGCGGAAAGCCCGGATTATTTTGTGGATTTTCCGGTGCCGTTTTGCGCGAGCTGGACCCCGCAGAGCAGCCTGATCAGCATTTATAGCCAGTTTCTGGCCGCGATTGAAAGCCTGAAAGCGTTTTGGGATGTGATGGATGAAATCGATGAAAAAACCTGGGTGCTGGAACCGGAAAAACCGCCGCGTAGCGCGACCGCGCGTCGTATTGCGCTGGGCAACAACGTGAGCATTAACATTGAAGTGGACCCGCGTCATCCGACCATGCTGCCGGAATGCTTTTTTCTGGGCGCGGATCATGTGGTGAAACCGCTGGGCATTAAACTGAGCCGTAACATTCATCTGTGGGACCCGGAAAACAGCGTGCTGCAGAACCTGAAAGATGTGCTGGAAATTGATTTTCCGGCGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHENLYFQMDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAVLKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQD
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDAS
GREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRH
PTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU53030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Propagate at 30 degrees