cDNA Clone CFLAR isoform 1 (FLIP-L)

• Synonyms: CASP8 and FADD-like apoptosis regulator isoform 1 [Homo sapiens]. FLIP-L
• Plasmid: pGEX6P HALO Thr-FLIP-L CFLAR isoform 1
• Parent Plasmid: pGEX 3C HALO Thr
• Expressed: GST-HALO-CFLAR
• Genbank: NM_003879.5 U97074.1
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GCGGCCGCGTatgtctgctgaagtcatccatcaggttgaagaagcacttg
atacagatgagaaggagatgctgctctttttgtgccgggatgttgctata
gatgtggttccacctaatgtcagggaccttctggatattttacgggaaag
aggtaagctgtctgtcggggacttggctgaactgctctacagagtgaggc
gatttgacctgctcaaacgtatcttgaagatggacagaaaagctgtggag
acccacctgctcaggaaccctcaccttgtttcggactatagagtgctgat
ggcagagattggtgaggatttggataaatctgatgtgtcctcattaattt
tcctcatgaaggattacatgggccgaggcaagataagcaaggagaagagt
ttcttggaccttgtggttgagttggagaaactaaatctggttgccccaga
tcaactggatttattagaaaaatgcctaaagaacatccacagaatagacc
tgaagacaaaaatccagaagtacaagcagtctgttcaaggagcagggaca
agttacaggaatgttctccaagcagcaatccaaaagagtctcaaggatcc
ttcaaataacttcaggctccataatgggagaagtaaagaacaaagactta
aggaacagcttggcgctcaacaagaaccagtgaagaaatccattcaggaa
tcagaagcttttttgcctcagagcatacctgaagagagatacaagatgaa
gagcaagcccctaggaatctgcctgataatcgattgcattggcaatgaga
cagagcttcttcgagacaccttcacttccctgggctatgaagtccagaaa
ttcttgcatctcagtatgcatggtatatcccagattcttggccaatttgc
ctgtatgcccgagcaccgagactacgacagctttgtgtgtgtcctggtga
gccgaggaggctcccagagtgtgtatggtgtggatcagactcactcaggg
ctccccctgcatcacatcaggaggatgttcatgggagattcatgccctta
tctagcagggaagccaaagatgttttttattcagaactatgtggtgtcag
agggccagctggaggacagcagcctcttggaggtggatgggccagcgatg
aagaatgtggaattcaaggctcagaagcgagggctgtgcacagttcaccg
agaagctgacttcttctggagcctgtgtactgcggacatgtccctgctgg
agcagtctcacagctcaccatccctgtacctgcagtgcctctcccagaaa
ctgagacaagaaagaaaacgcccactcctggatcttcacattgaactcaa
tggctacatgtatgattggaacagcagagtttctgccaaggagaaatatt
atgtctggctgcagcacactctgagaaagaaacttatcctctcctacaca
taagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSPEFPGRLERPRMSA
EVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVG
DLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEIGED
LDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLE
KCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRL
HNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGI
CLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHR
DYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPK
MFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEFKAQKRGLCTVHREADFFW
SLCTADMSLLEQSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYMYDW
NSRVSAKEKYYVWLQHTLRKKLILSYT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours silent mutation nt a1260g (silent at P420) compared to ref sequence shown
• Low Temp: No