cDNA Clone hiw

• Synonyms: RING-type E3 ubiquitin transferase highwire, highwire
• Plasmid: pGEX6P-1-hiw aa4979-5233
• Parent Plasmid: pGEX6P-1
• Expressed: GST-hiw aa4979-5233
• Genbank: Gene 32429 Q9NB71 NM_078604.4 → NP_511159.3
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCGATCCGAAGCTCACCCAGGATGCGGACGACATGTGCATGATATGCTTCGTTGAGGCATTGTCCTGTGCACCATCAATACATCTGGAGTGCGGTCACGTGTTCCACTATCATTGCTGCAAGGCGGTTCTGGAGAAGCGCTGGAGCGGACCACGCATCACATTTGGCTTCTCCCTGTGTCCCATCTGCAAGGCGGACATCCAGCATCCCCTGCTCTCAGATATCCTGGAGCCGATCAATGGCCTCAAACAGGACGTCAAACGCAAAGCTCTCATGCGGATCAAATACGAGGGCGTAGTAAAAGACACGGACAGCAAAAACGTCAACATGACCCAGCTGGCAATGGATCGATATGCCTATTATGTGTGCTTTAAATGCCAGAAGGCGTACTACGGCGGCGAGGCGCGCTGTGACGCGGAAATTGGCGAGAAGTTCGATCCGGAGGAGCTGGTCTGCGGCGGTTGCTCGGATGTGGCCCGGGCACAGATGTGTCCGAAGCATGGCACCGATTTCTTGGAGTACAAGTGCCGCTACTGCTGCTCGGTGGCGGTATTCTTCTGCTTTGGAACCACACACTTCTGTGACACCTGCCACGACGATTTCCAGCGACTGACCAATATACCCAAGGTCAAGTTGCCCCAGTGCCCAGCCGGACCGAAGGCCAAGCAGTTGCTGGGCGACGAATGTCCGCTGCACGTAATGCATCCGCCCACTGGCGAGGAGTTCGCCCTCGGCTGCGGCGTCTGTCGCAATGCCCAAACATTTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDPKLTQDADDMCMICFVEA
LSCAPSIHLECGHVFHYHCCKAVLEKRWSGPRITFGFSLCPICKADIQHP
LLSDILEPINGLKQDVKRKALMRIKYEGVVKDTDSKNVNMTQLAMDRYAY
YVCFKCQKAYYGGEARCDAEIGEKFDPEELVCGGCSDVARAQMCPKHGTD
FLEYKCRYCCSVAVFFCFGTTHFCDTCHDDFQRLTNIPKVKLPQCPAGPK
AKQLLGDECPLHVMHPPTGEEFALGCGVCRNAQTF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28887
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Drosophila orthologue of MYCBP2. Isoform A
• Low Temp: No