cDNA Clone hiw

• Synonyms: RING-type E3 ubiquitin transferase highwire, highwire
• Plasmid: pGEX6P-1-hiw aa4979-5233 C5115S
• Parent Plasmid: pGEX6P-1
• Expressed: GST-hiw aa4979-5233 C5115S
• Genbank: Gene 32429 Q9NB71 NM_078604.4 → NP_511159.3
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCGATCCGAAGCTCACCCAGGATGCGGACGACATGTGCATGATATG
CTTCGTTGAGGCATTGTCCTGTGCACCATCAATACATCTGGAGTGCGGTC
ACGTGTTCCACTATCATTGCTGCAAGGCGGTTCTGGAGAAGCGCTGGAGC
GGACCACGCATCACATTTGGCTTCTCCCTGTGTCCCATCTGCAAGGCGGA
CATCCAGCATCCCCTGCTCTCAGATATCCTGGAGCCGATCAATGGCCTCA
AACAGGACGTCAAACGCAAAGCTCTCATGCGGATCAAATACGAGGGCGTA
GTAAAAGACACGGACAGCAAAAACGTCAACATGACCCAGCTGGCAATGGA
TCGATATGCCTATTATGTGTGCTTTAAATGCCAGAAGGCGTACTACGGCG
GCGAGGCGCGCTcTGACGCGGAAATTGGCGAGAAGTTCGATCCGGAGGAG
CTGGTCTGCGGCGGTTGCTCGGATGTGGCCCGGGCACAGATGTGTCCGAA
GCATGGCACCGATTTCTTGGAGTACAAGTGCCGCTACTGCTGCTCGGTGG
CGGTATTCTTCTGCTTTGGAACCACACACTTCTGTGACACCTGCCACGAC
GATTTCCAGCGACTGACCAATATACCCAAGGTCAAGTTGCCCCAGTGCCC
AGCCGGACCGAAGGCCAAGCAGTTGCTGGGCGACGAATGTCCGCTGCACG
TAATGCATCCGCCCACTGGCGAGGAGTTCGCCCTCGGCTGCGGCGTCTGT
CGCAATGCCCAAACATTTTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDPKLTQDADDMCMICFVEA
LSCAPSIHLECGHVFHYHCCKAVLEKRWSGPRITFGFSLCPICKADIQHP
LLSDILEPINGLKQDVKRKALMRIKYEGVVKDTDSKNVNMTQLAMDRYAY
YVCFKCQKAYYGGEARSDAEIGEKFDPEELVCGGCSDVARAQMCPKHGTD
FLEYKCRYCCSVAVFFCFGTTHFCDTCHDDFQRLTNIPKVKLPQCPAGPK
AKQLLGDECPLHVMHPPTGEEFALGCGVCRNAQTF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28913
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Drosophila orthologue of MYCBP2. Isoform A
• Low Temp: No