cDNA Clone MAP3K12

• Synonyms: MAP3K12 mitogen-activated protein kinase kinase kinase 12 , DLK, MUK, ZPK, ZPKP1, MEKK12
• Plasmid: pSc-B-MAP3K12 short variant
• Parent Plasmid: pSC-B
• Genbank: 7786
• Species: Human

Sequence of Insert

CTCGAGATGGCTTGCCTCCATGAGACCCGAACACCCTCTCCTTCCTTTGGGGGCTT
TGTGTCTACCCTAAGTGAGGCATCCATGCGCAAGCTGGACCCAGACACTT
CTGACTGCACTCCCGAGAAGGACCTGACGCCTACCCAGTGTGTACTTCGA
GATGTGGTACCCCTTGGTGGGCAGGGTGGGGGAGGGCCCAGCCCCTCCCC
AGGTGGAGAGCCGCCCCCTGAGCCTTTTGCCAACAGTGTCCTGCAGCTAC
ATGAGCAGGATGCAGGGGGCCCAGGGGGAGCAGCTGGGTCACCTGAGAGT
CGGGCATCCAGAGTTCGAGCTGACGAGGTGCGACTGCAGTGCCAGAGTGG
CAGTGGCTTCCTTGAGGGCCTCTTTGGCTGCCTGCGCCCTGTCTGGACCA
TGATTGGCAAAGCCTACTCCACTGAGCACAAGCAGCAGCAGGAAGACCTT
TGGGAGGTCCCCTTTGAGGAAATCCTGGACCTGCAGTGGGTGGGCTCAGG
GGCCCAGGGTGCTGTCTTCCTGGGGCGCTTCCACGGGGAGGAGGTGGCTG
TGAAGAAGGTGCGAGACCTCAAAGAAACCGACATCAAGCACTTGCGAAAG
CTGAAGCACCCCAACATCATCACTTTCAAGGGTGTGTGCACCCAGGCTCC
CTGCTACTGCATCCTCATGGAGTTCTGCGCCCAGGGCCAGCTGTATGAGG
TACTGCGGGCTGGCCGCCCTGTCACCCCCTCCTTACTGGTTGACTGGTCC
ATGGGCATCGCTGGTGGCATGAACTACCTGCACCTGCACAAGATTATCCA
CAGGGATCTCAAGTCACCCAACATGCTAATCACCTACGACGATGTGGTGA
AGATCTCAGATTTTGGCACTTCCAAGGAGCTGAGTGACAAGAGCACCAAG
ATGTCCTTTGCAGGGACAGTAGCCTGGATGGCCCCTGAGGTGATCCGCAA
TGAACCTGTGTCTGAGAAGGTCGACATCTGGTCCTTTGGCGTGGTGCTAT
GGGAACTGCTGACTGGTGAGATCCCCTACAAAGACGTAGATTCCTCAGCC
ATTATCTGGGGTGTGGGAAGCAACAGTCTCCATCTGCCCGTGCCCTCCAG
TTGCCCAGATGGTTTCAAGATCCTGCTTCGCCAGTGCTGGAATAGCAAAC
CACGAAATCGCCCATCATTCCGACAGATCCTGCTGCATCTGGACATTGCC
TCAGCTGATGTACTCTCCACACCCCAGGAGACTTACTTTAAGTCCCAGGC
AGAGTGGCGGGAAGAAGTAAAACTGCACTTTGAAAAGATTAAGTCAGAAG
GGACCTGTCTGCACCGCCTAGAAGAGGAACTGGTGATGAGGAGGAGGGAG
GAGCTCAGACACGCCCTGGACATCAGGGAGCACTATGAAAGGAAGCTGGA
GAGAGCCAACAACCTGTATATGGAACTTAATGCCCTCATGTTGCAGCTGG
AACTCAAGGAGAGGGAGCTGCTCAGGCGAGAGCAAGCTTTAGAGCGGAGG
TGCCCAGGCCTGCTGAAGCCACACCCTTCCCGCGGCCCCCGAGCTTCCCT
CCCTCCATGAGCGGCCGC

Amino Acid Sequence

MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLR
DVVPLGGQGGGGPSPSPGGEPPPEPFANSVLQLHEQDAGGPGGAAGSPES
RASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDL
WEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRK
LKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWS
MGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTK
MSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSA
IIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIA
SADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRRE
ELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERR
CPGLLKPHPSRGPRASLPP*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28968
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: M1-G512 of isoform 1 intact plus the extreme C-terminal 7 residues of isoform 1; Clone contains 1.1kb deletion just in from the extreme C-terminus ( Leu513-Pro886 with a substitution P887>R) compared to NCBI ref isoform 1 NM_001193511.1 → NP_001180440.1
• Low Temp: No