cDNA Clone MYCBP2

• Plasmid: pSc-B-MYCBP2-Val2340-Phe4640 C4520A
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

ctcgagGTTTATGAAAATTATTCATTTGAAGAACTACGTTTTGCATCACC
AACTCCTAAGAGACCCAGTGAGAATATGCTGATCCGTGTCAATAATGATG
GGACTTATTGTGCAAATTGGACTCCAGGGGCTATTGGACTCTACACTCTT
CATGTTACCATTGATGGCATTGAAATCGATGCTGGTCTGGAAGTAAAAGT
AAAAGACCCACCAAAAGGGATGATACCACCAGGAACTCAGTTGGTCAAAC
CAAAGTCTGAACCTCAGCCTAATAAGGTTCGAAAATTTGTGGCCAAGGAC
AGTGCGGGGCTTCGCATCCGTAGCCACCCTTCCCTTCAGAGTGAGCAGAT
AGGCATAGTGAAAGTCAATGGAACTATCACTTTTATTGATGAGATCCATA
ATGATGATGGTGTGTGGCTGAGGCTGAATGATGAGACAATAAAGAAGTAT
GTCCCTAACATGAATGGTTACACTGAAGCCTGGTGCCTCTCTTTTAATCA
ACATCTTGGCAAGAGTCTTCTGGTCCCTGTTGACGAATCTAAAACTAATA
CTGATGACTTTTTCAAAGACATAAACTCCTGCTGCCCACAGGAAGCAACA
ATGCAAGAACAAGATATGCCATTCTTGCGAGGAGGGCCAGGCATGTACAA
GGTAGTGAAGACGGGACCTTCAGGTCACAACATCAGAAGCTGCCCTAACC
TTAGAGGTATCCCAATTGGAATGTTAGTTCTGGGAAACAAAGTCAAAGCA
GTGGGAGAGGTAACCAATTCTGAAGGGACATGGGTGCAACTGGATCAGAA
CAGCATGGTAGAGTTCTGTGAGAGTGATGAAGGAGAGGCATGGTCCTTAG
CTAGAGACAGAGGCGGAAACCAGTACCTCCGACATGAAGATGAACAAGCT
CTTCTGGATCAGAATTCTCAAACTCCTCCTCCAAGCCCTTTCTCAGTGCA
AGCTTTTAATAAAGGGGCAAGTTGCAGTGCCCAAGGATTTGATTATGGAC
TCGGAAATAGCAAAGGTGATCGAGGAAACATCTCAACATCTTCTAAACCA
GCCTCTACATCAGGAAAATCAGAGCTGTCCTCTAAACACAGCAGATCGCT
TAAACCTGATGGACGTATGAGCCGGACTACTGCTGATCAGAAGAAGCCAA
GGGGCACAGAAAGTTTATCTGCTAGTGAATCCCTCATCTTAAAATCTGAT
GCTGCAAAGTTGAGGTCAGATTCCCACAGTAGGTCATTATCCCCCAACCA
TAACACCTTGCAGACATTGAAATCTGATGGGAGGATGCCTTCTAGCTCCA
GAGCTGAATCCCCAGGACCAGGTTCTCGGTTGTCGTCTCCTAAGCCAAAG
ACTCTCCCAGCCAATAGGTCTAGCCCATCGGGTGCTAGTTCTCCACGCTC
CTCCTCACCACATGATAAAAATCTACCTCAAAAAAGTACTGCTCCTGTTA
AGACAAAGCTTGATCCTCCTCGGGAACGTTCTAAATCAGACTCTTACACA
CTTGATCCAGATACCCTCCGCAAGAAGAAAATGCCCCTCACAGAACCTTT
GAGAGGACGGTCAACGTCACCAAAACCAAAATCAGTACCAAAGGATTCTA
CAGATTCCCCTGGATCTGAAAATAGAGCTCCCTCTCCCCATGTGGTACAG
GAAAACCTCCACAGTGAGGTGGTCGAAGTCTGCACCTCAAGTACTTTAAA
AACAAATAGTCTAACAGACAGCACCTGCGATGACAGCAGTGAATTTAAGA
GTGTGGATGAAGGTTCAAATAAAGTTCATTTTAGCATTGGAAAAGCACCA
CTGAAAGATGAACAGGAAATGAGAGCATCTCCCAAAATAAGTCGAAAATG
TGCTAATAGACACACCAGGCCCAAAAAAGAAAAATCGAGTTTTCTTTTCA
AAGGAGATGGATCCAAGCCTTTAGAGCCAGCCAAGCAAGCCATGTCTCCT
TCTGTGGCCGAATGTGCCAGAGCTGTGTTTGCTTCCTTCCTCTGGCATGA
AGGCATAGTACATGATGCAATGGCTTGTTCTTCTTTCCTAAAGTTTCATC
CTGAACTTTCCAAAGAACATGCTCCTATAAGGAGTAGTTTAAATAGCCAA
CAACCTACAGAGGAAAAAGAAACCAAGTTAAAAAATAGACATTCATTAGA
AATATCATCTGCACTGAATATGTTTAATATTGCACCCCATGGACCAGATA
TATCTAAGATGGGTAGCATCAACAAAAACAAGGTATTGTCTATGCTTAAG
GAACCACCTCTGCATGAAAAATGTGAGGATGGGAAAACCGAGACCACTTT
TGAAATGTCCATGCATAACACAATGAAGTCTAAGTCTCCTCTTCCCTTAA
CTTTACAACATTTAGTGGCTTTTTGGGAAGACATCTCTTTGGCTACTATC
AAAGCTGCTTCCCAGAATATGATTTTTCCAAGTCCTGGTTCCTGTGCAGT
TCTTAAAAAGAAAGAGTGTGAGAAAGAGAATAAGAAGTCCAAAAAGGAAA
AAAAGAAAAAAGAAAAGGCAGAAGTTAGGCCCAGGGGTAATTTGTTTGGA
GAGATGGCCCAGCTGGCAGTAGGAGGACCAGAGAAAGATACCATCTGTGA
ACTGTGTGGGGAGTCACATCCATACCCGGTGACCTATCACATGAGACAAG
CTCACCCAGGTTGTGGCCGATATGCTGGTGGACAAGGTTACAATAGCATT
GGGCATTTTTGTGGAGGATGGGCTGGTAACTGTGGTGATGGTGGCATAGG
AGGAAGCACTTGGTATCTGGTATGTGATCGCTGTAGAGAAAAATACCTCC
GCGAAAAACAGGCTGCTGCAAGGGAGAAGGTCAAACAATCTAGGAGAAAG
CCAATGCAAGTCAAGACCCCTCGTGCCTTGCCCACCATGGAAGCTCACCA
GGTGATTAAAGCCAATGCACTCTTCCTGCTGTCCCTGAGCAGTGCAGCAG
AACCGAGCATTCTGTGTTACCATCCTGCAAAGCCATTCCAATCTCAGTTG
CCCAGTGTAAAAGAAGGCATTTCTGAGGATCTTCCTGTGAAAATGCCTTG
TCTCTACCTGCAGACATTAGCTAGGCATCATCATGAAAATTTTGTGGGCT
ATCAAGATGACAATCTATTCCAGGATGAAATGAGATATCTACGTTCAACA
TCTGTACCTGCCCCGTATATATCAGTAACTCCTGATGCAAGTCCTAATGT
ATTTGAAGAGCCAGAGAGCAATATGAAGTCTATGCCACCAAGTTTAGAAA
CCAGTCCCATAACTGATACTGATCTTGCAAAGAGAACTGTCTTCCAAAGA
TCATACTCAGTTGTTGCTTCCGAATATGATAAACAACACTCCATTTTACC
TGCACGAGTTAAAGCTATTCCTAGAAGAAGAGTTAACAGTGGAGACACTG
AAGTTGGTTCTTCCCTTTTGAGACATCCGTCTCCTGAGCTTTCTCGGCTA
ATCTCAGCCCACAGCTCTCTTTCTAAAGGAGAACGAAATTTCCAGTGGCC
AGTTTTAGCTTTTGTTATACAACATCATGATCTAGAAGGTCTTGAAATAG
CAATGAAACAGGCCCTAAGGAAATCTGCTTGTCGAGTTTTTGCTATGGAG
GCTTTCAACTGGCTTCTGTGTAATGTCATCCAAACCACTTCTCTCCATGA
TATTCTGTGGCATTTTGTGGCATCACTGACTCCTGCACCAGTGGAACCAG
AGGAAGAAGAGGATGAAGAAAATAAAACAAGCAAAGAAAATTCAGAACAA
GAGAAAGATACAAGAGTATGTGAACATCCACTCTCAGACATAGTGATTGC
CGGGGAAGCTGCTCATCCTTTACCACACACCTTTCACCGCTTGCTGCAGA
CCATCTCAGACCTTATGATGTCTCTCCCCAGCGGCAGTTCATTACAGCAA
ATGGCCCTGAGGTGCTGGAGTCTCAAATTCAAGCAATCTGATCACCAGTT
CCTTCATCAGAGCAACGTCTTTCATCACATTAACAATATTTTGTCAAAGT
CAGATGATGGCGATAGTGAAGAGAGTTTTAGCATCAGTATACAGTCTGGC
TTTGAAGCTATGAGTCAGGAATTATGCATAGTAATGTGCTTAAAGGACTT
AACCAGCATTGTTGACATAAAAACTTCAAGCCGACCTGCCATGATTGGCA
GTTTGACAGACGGCTCCACAGAAACCTTTTGGGAATCAGGAGATGAAGAT
AAAAACAAAACTAAGAACATCACCATCAACTGTGTAAAAGGAATCAATGC
CCGCTATGTGTCTGTTCACGTGGACAATTCCCGAGATCTTGGGAATAAAG
TTACCTCAATGACCTTCTTAACTGGCAAAGCAGTAGAAGATTTGTGCAGA
ATAAAGCAGGTTGATCTGGATTCCAGGCACATTGGCTGGGTAACAAGTGA
ACTTCCAGGAGGGGATAATCACATCATAAAAATTGAATTAAAAGGCCCAG
AAAATACACTGAGAGTTCGACAAGTCAAAGTCCTGGGCTGGAAAGATGGT
GAAAGCACAAAAATAGCTGGCCAGATTTCAGCCAGTGTGGCCCAGCAGAG
GAACTGTGAAGCTGAGACTCTGCGAGTATTCAGACTGATTACGTCTCAAG
TATTTGGAAAGCTCATCTCTGGAGATGCTGAACCTACACCAGAACAAGAG
GAAAAAGCACTATTGTCATCACCTGAAGGAGAAGAAAAAGTATACAATGC
AACATCAGATGCTGACCTGAAAGAACATATGGTTGGAATCATATTCAGCA
GGAGTAAGCTGACTAACTTACAAAAACAGGTGTGTGCTCATATTGTCCAA
GCTATTCGCATGGAAGCTACCAGAGTCCGTGAAGAATGGGAACATGCTAT
ATCAAGCAAAGAAAATGCCAATTCTCAGCCAAATGATGAAGATGCCTCCT
CTGATGCCTACTGCTTTGAGCTGCTCTCTATGGTTTTAGCACTGAGTGGC
TCTAACGTTGGCCGGCAATATCTGGCTCAACAGCTAACCCTGCTTCAGGA
TCTCTTCTCGCTGCTTCACACAGCCTCTCCTAGAGTCCAGAGACAGGTAA
CCTCTTTACTAAGAAGAGTTTTGCCTGAAGTAACCCCTAGTCGTCTGGCC
AGCATCATAGGAGTGAAATCCCTCCCCCCAGCAGATATCAGTGATATCAT
TCACTCAACAGAGAAAGGAGACTGGAATAAGCTGGGTATCTTGGACATGT
TTCTAGGATGCATTGCCAAAGCACTCACTGTACAGCTAAAAGCCAAAGGA
ACCACCATCACTGGAACAGCTGGTACCACTGTGGGCAAAGGAGTTACAAC
AGTTACTCTTCCGATGATTTTCAATTCCAGTTATCTCCGACGAGGTGAAA
GTCATTGGTGGATGAAGGGCTCAACCCCTACCCAGATCTCAGAGATCATC
ATTAAACTTATCAAGGATATGGCAGCAGGTCATCTGTCAGAAGCTTGGTC
CCGAGTGACAAAAAATGCTATTGCAGAAACCATCATTGCCTTGACCAAGA
TGGAAGAAGAATTTAGGTCTCCAGTGAGATGTATTGCAACAACTAGACTC
TGGCTTGCTCTCGCATCCCTATGTGTTCTTGATCAGGACCACGTAGATCG
TCTCTCCTCGGGGAGATGGATGGGAAAGGATGGACAACAAAAACAAATGC
CTATGTGTGATAACCATGATGATGGTGAAACTGCAGCAATCATTTTATGC
AATGTCTGTGGAAATTTATGTACAGACTGTGACAGATTCCTTCACCTTCA
TCGAAGAACCAAAACTCATCAAAGACAGGTCTTCAAAGAAGAAGAAGAAG
CTATAAAGGTTGACCTTCATGAAGGTTGTGGTAGAACCAAATTGTTCTGG
TTGATGGCACTGGCAGATTCTAAAACAATGAAGGCAATGGTGGAATTCCG
AGAACACACAGGCAAACCCACCACGAGTAGCTCAGAAGCATGTCGCTTCT
GTGGTTCCAGGAGTGGAACAGAGTTATCTGCTGTTGGCAGTGTTTGTTCT
GATGCAGATTGCCAGGAATACGCTAAGATAGCCTGTAGTAAGACGCATCC
TTGTGGCCATCCATGCGGGGGTGTTAAAAACGAAGAGCACTGTCTGCCCT
GTCTACACGGCTGTGACAAAAGTGCCACAAGCCTGAAGCAAGACGCCGAT
GACATGTGCATGATATGTTTCACCGAAGCGCTCTCGGCAGCACCAGCCAT
TCAGCTGGATTGTAGTCACATATTCCACTTACAGTGCTGTCGGCGAGTAT
TAGAAAATCGATGGCTTGGCCCAAGGATAACATTTGGATTTATATCTTGT
CCCATTTGCAAGAACAAAATTAATCACATAGTACTAAAAGACCTACTTGA
TCCAATAAAAGAACTCTATGAGGATGTCAGAAGAAAAGCCTTAATGAGAT
TGGAATATGAAGGTCTGCATAAGAGTGAAGCTATCACAACTCCTGGTGTG
AGGTTTTATAATGACCCAGCTGGCTATGCAATGAATAGATATGCATATTA
TGTGTGCTACAAATGCAGAAAGGCATATTTTGGTGGTGAAGCTCGCgcCG
ATGCTGAGGCTGGACGGGGAGATGATTATGATCCCAGAGAGCTCATTTGT
GGTGCCTGTTCTGATGTTTCCAGGGCTCAGATGTGTCCCAAACATGGCAC
AGACTTTTTGGAATATAAATGTCGCTACTGCTGTTCAGTGGCTGTTTTTT
TCTGTTTTGGAACAACACATTTTTGTAATGCTTGTCATGATGATTTTCAA
AGAATGACTAGCATTCCTAAGGAAGAACTACCACACTGTCCTGCAGGTCC
CAAAGGCAAGCAGTTAGAAGGAACTGAATGTCCACTCCATGTTGTTCATC
CACCCACTGGGGAAGAGTTTGCTCTGGGATGTGGAGTGTGCAGAAATGCC
CACACTTTTTAGgcggccgc

Amino Acid Sequence

VYENYSFEELRFASPTPKRPSENMLIRVNNDGTYCANWTPGAIGLYTLHV
TIDGIEIDAGLEVKVKDPPKGMIPPGTQLVKPKSEPQPNKVRKFVAKDSA
GLRIRSHPSLQSEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVP
NMNGYTEAWCLSFNQHLGKSLLVPVDESKTNTDDFFKDINSCCPQEATMQ
EQDMPFLRGGPGMYKVVKTGPSGHNIRSCPNLRGIPIGMLVLGNKVKAVG
EVTNSEGTWVQLDQNSMVEFCESDEGEAWSLARDRGGNQYLRHEDEQALL
DQNSQTPPPSPFSVQAFNKGASCSAQGFDYGLGNSKGDRGNISTSSKPAS
TSGKSELSSKHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAA
KLRSDSHSRSLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTL
PANRSSPSGASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLD
PDTLRKKKMPLTEPLRGRSTSPKPKSVPKDSTDSPGSENRAPSPHVVQEN
LHSEVVEVCTSSTLKTNSLTDSTCDDSSEFKSVDEGSNKVHFSIGKAPLK
DEQEMRASPKISRKCANRHTRPKKEKSSFLFKGDGSKPLEPAKQAMSPSV
AECARAVFASFLWHEGIVHDAMACSSFLKFHPELSKEHAPIRSSLNSQQP
TEEKETKLKNRHSLEISSALNMFNIAPHGPDISKMGSINKNKVLSMLKEP
PLHEKCEDGKTETTFEMSMHNTMKSKSPLPLTLQHLVAFWEDISLATIKA
ASQNMIFPSPGSCAVLKKKECEKENKKSKKEKKKKEKAEVRPRGNLFGEM
AQLAVGGPEKDTICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGH
FCGGWAGNCGDGGIGGSTWYLVCDRCREKYLREKQAAAREKVKQSRRKPM
QVKTPRALPTMEAHQVIKANALFLLSLSSAAEPSILCYHPAKPFQSQLPS
VKEGISEDLPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSV
PAPYISVTPDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSY
SVVASEYDKQHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLIS
AHSSLSKGERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAF
NWLLCNVIQTTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEK
DTRVCEHPLSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMA
LRCWSLKFKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFE
AMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKN
KTKNITINCVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIK
QVDLDSRHIGWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGES
TKIAGQISASVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEK
ALLSSPEGEEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAI
RMEATRVREEWEHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSN
VGRQYLAQQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASI
IGVKSLPPADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTT
ITGTAGTTVGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIK
LIKDMAAGHLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWL
ALASLCVLDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNV
CGNLCTDCDRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLM
ALADSKTMKAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDA
DCQEYAKIACSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDM
CMICFTEALSAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPI
CKNKINHIVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRF
YNDPAGYAMNRYAYYVCYKCRKAYFGGEARADAEAGRGDDYDPRELICGA
CSDVSRAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRM
TSIPKEELPHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHT
F*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29005
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K2403 and H3239 restored. Clone has two silent mutations at K3288 (AAA > AAG) and S2782 (TCA > TCG) relative to NCBI ref.; Clone has a shorter N-terminus than NCBI ref NM_015057.4 such that Val2340-Phe4640 here actually corresponds to V2378-F4678 of full-length NM_015057.4
• Low Temp: No