cDNA Clone MAP4K2

• Plasmid: pGEX6P1 MAP4K2 444-820
• Parent Plasmid: pGEX6P1
• Expressed: GST MAP4K2 444-820
• Genbank: NM_004579.4* see notes
• Tag: GST
• Species: Human

Sequence of Insert

CTCGAGATTTCCGGCCTGGAAGTTCTGTTCCAGGGGCCCGGGGATCCCCGGAATTCCCGGGTCGACGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTG
AGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGC
TGTCACCTGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACG
CTGGAGAAGCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACC
TCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCT
GTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGC
CTGCTCCTGCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGG
TGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGG
CCTGACGCCCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGC
TGTGTGAGGATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACA
GTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCT
TGGGGCCCACAGAGACATCATCCGGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAA
GCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAE
EGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRN
PYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQ
VIQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETRIFRVLGAHRDIIRESIPTDNPEA
HSNLYILTGHQSTY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56472
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Has change L976R compared with NM_004579.4. DU58696 has this corrected to wild-type Leucine.
• Low Temp: No