cDNA Clone DD1

• Plasmid: pGEX C3 HALO thrombin-DD1
• Parent Plasmid: pGEX C3 HALO thrombin
• Expressed: GST-HALO-DD1
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGCTGATCACCGTGTACTGCGTGCGGAGGGACCTCTCCGAGGTCACCTTCTCTCTCCAGGTCAGCCCCGACTTTGAGCTCCGAAACTTCAAGGTCCTCTGCGAAGCGGAGTCCAGAGTCCCCGTCGAAGAGATCCAGATCATCCACATGGAGCGACTCCTCATCGAGGACCACTGTTCCCTGGGCTCCTACGGCCTCAAAGATGGCGATATCGTGGTTTTACTGCAGAAGGACAATGTGGGACCTCGGGCTCCAGGGCGTGCCCCGAACCAGCCTCGTGTAGACTTCAGTGGCATTGCGGTGCCTGGGACGTCCAGCTCCCGTCCACAGCACCCTGGACAGCAGCAGCAGCGCACACCCGCTGCCCAGCGGTCACAGGGCTTGGCGTCAGGAGAGAAGGTGGCCGGCCTGCAAGGTCTGGGCAGCCCCGCCCTGATCCGCAGCATGCTGCTCTCCAACCCCCACGATCTGTCCCTGCTCAAGGAACGCAACCCTCCCTTGGCGGAAGCCCTGCTCAGCGGAAGCCTTGAGACCTTTTCTCAGGTGCTGATGGAGCAGCAAAGGGAAAAGGCCTTGAGAGAGCAAGAGAGGCTTCGTCTCTACACAGCCGACCCACTGGATCGGGAAGCTCAGGCCAAAATAGAAGAGGAAATCCGGCAGCAAAACATTGAAGAAAACATGAATATAGCGATAGAAGAGGCCCCCGAGAGTTTTGGACAAGTGACGATGCTCTACATTAACTGCAAAGTGAATGGGCATCCTTTGAAGGCTTTTGTTGACTCGGGCGCCCAGATGACCATTATGAGCCAGGCTTGTGCCGAGCGATGTAACATCATGAGGCTGGTGGACCGACGGTGGGCTGGGGTTGCTAAAGGAGTGGGCACACAGAGAATTATTGGCCGTGTTCATCTAGCTCAGATTCAAATTGAAGGTGATTTCTTACAGTGCTCTTTCTCCATACTTGAGGATCAACCCATGGATATGCTTCTAGGCCTAGATATGCTCCGGAGACATCAATGTTCCATCGATTTGAAGAAAAATGTGCTGGTCATCGGCACCACTGGCACGCAGACTTATTTTCTTCCTGAGGGAGAGTTGCCCTTATGCTCTAGGATGGTAAGTGGGCAAGATGAGTCTTCGGACAAGGAAATTACACATTCAGTCATGGATTCAGGACGAAAAGAGCATTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMLITVYCVRRDLSE
VTFSLQVSPDFELRNFKVLCEAESRVPVEEIQIIHMERLLIEDHCSLGSY
GLKDGDIVVLLQKDNVGPRAPGRAPNQPRVDFSGIAVPGTSSSRPQHPGQ
QQQRTPAAQRSQGLASGEKVAGLQGLGSPALIRSMLLSNPHDLSLLKERN
PPLAEALLSGSLETFSQVLMEQQREKALREQERLRLYTADPLDREAQAKI
EEEIRQQNIEENMNIAIEEAPESFGQVTMLYINCKVNGHPLKAFVDSGAQ
MTIMSQACAERCNIMRLVDRRWAGVAKGVGTQRIIGRVHLAQIQIEGDFL
QCSFSILEDQPMDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTYFLPEG
ELPLCSRMVSGQDESSDKEITHSVMDSGRKEH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29233
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 24192
• Low Temp: No