cDNA Clone MAP4K2 (human)

• Plasmid: pFastBac-6His-TEV-HALO-C3-MAP4K2
• Parent Plasmid: pFastBac-6His-TEV-HALO-C3
• Genbank: NM_004579.4
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GTCGACGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTTCGAGCTGCTGCAGCGTGTGGGGGCCGGGACCTATGGCGACGTCTACAAGGCCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTAGACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAGGAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGTCTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGATCCACAGAGACATCAAGGGGGCCAACCTTCTCCTCACTCTCCAGGGAGATGTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGCCAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCCCTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCACCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGCCGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTCTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAAGCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCACACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCTGAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTCCCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTCACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATGAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTATTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATGGGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCCAGCAGAGGAGCCTCTGTCCAGTCCCCCAGGAACCCTGCCCCCACCTCCTTCAGGCCCCAACAGCTCCCCACTGCTGCCCACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCTGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGCCCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAGGATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCCACAGAGACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMAEIGTGFPFDPHYVEVLGERMHYV
DVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKP
DLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKG
IAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPM
GVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEY
MDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQ
EDNPDLIGSEIARWLSTLEISGLEVLFQGPGSGIQRPTSTRCCGMCRCRT
RGTASSCCSVWGPGPMATSTRPATRSRPNWPP*R*SS*TQGTTSAPSSRK
SPSCVSAATPMWWPTLAATSGMTACGSAWSSAEGAPCRRFTMPLGPWRSG
RLPTSAERH*RGSTTCILRGRSTETSRGPTFSSLSREMSNWLTLGCQAS*
QRLWPRGGLSLGLPTGWLPRWLLWSAKVATMSYVTSGPWASLPLSWASCS
PLCSTCTP*GP*CSCRRAASSRPN*EIRLAGPRISTTFSNWP*PRILRRG
RQQRSSCSTRSRLSSSLGPSSHSCWTKPVTLIWGPPPLRTVSWRPMTCFQ
TPFTPGGSTAQPRGPPRRSSFTR*NLAPHAGRKLTH*MSRGRKSGHYWER
KS*VGACCSRSRRPWRKGV*LFGQPQNSRSWTPQTIPWEPSSGPRS*GHS
PLTLQQRSLCPVPQEPCPHLLQAPTAPHCCPRPGPP*SSGRILRGHPATG
SPQLPRCIWAPASPRSSMAAPCGSTLLSPGFTLLLGTSSWW*GPRKASTH
STCMNCMRIRWRS*FHIAAPGSTA*TTCCCHSQGNPRTSGPMTSQACLSS
GGYSNRFPSPSPPTASPSASSPGALLCPPRFLTPKAACSVVWCGTPTRVP
PSCWPPCPPACSCCSGMSRCRSFCC*RTSPALCPAQLGCWSRWCWMGRSC
RRCVLGPRGLRGPAAASCSMSCPWRLA*RPTSSSHLRGSQARPSR*SRWT
GTQS*SALNAV*GLSTCRASPRPHWHLS*PLISPSRLWCACRTVCWPSGA
MGCKAEAWIPMR*PRRSQMKQGSSECLGPTETSSWRAFPLTTQRRTATST
SSRATRAPT

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU29312
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FRAMESHIFT MUTANT - oligos ordered to correct
• Low Temp: No