cDNA Clone CHFR

• Synonyms: E3 ubiquitin-protein ligase CHFR isoform 1, RNF116, RNF196, checkpoint with forkhead and ring finger domains
• Plasmid: pGEX6P-1-CHFR isoform 1
• Parent Plasmid: pGEX6P-1
• Expressed: GST-CHFR isoform 1
• Genbank: NM_001161344.1 → NP_001154816.1
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCC
CTGGGGACGGCTCCTGCGTCTGGGCGCGGAGGAGGGCGAGCCGCACGTCC
TCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGACCTT
TCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGT
GGATGAAAAATCAGGTCAGGTGACACTGGAAGATACCAGCACCAGTGGAA
CAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGACATGCCCTTTACAG
ACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAA
CGTGGCATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAG
AATCCTTTGAAGCTAACAAGGAAAATGTGTTCCATGGGACCAAAGATACC
TCAGGTGCAGGTGCAGGGCGAGGGGCCGATCCCCGGGTCCCTCCGTCGTC
GCCCGCCACTCAGGTGTGCTTTGAGGAACCACAGCCATCAACATCGACGT
CAGACCTCTTCCCCACAGCCTCGGCCTCTTCCACGGAGCCTTCTCCTGCA
GGGCGAGAGCGTTCCTCCAGTTGTGGGTCTGGGGGTGGTGGCATCTCCCC
TAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTCCAGCTTTGCCT
CAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAG
GATCAGGAGGATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGA
CCTTGACCTGAACGGGCAGTTGTTGGTCGCACAACCGCGTAGAAATGCCC
AAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGATG
GAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGT
GAGTTTGCAGCCCTGCATGCACACGTTCTGCGCGGCTTGCTACTCGGGCT
GGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTCCCGTGGAGCGG
ATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCA
GCATCCAGACAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCA
GGAATAAAATCACTCAAGACATGCTGCAGCCCAAAGTCAGGCGGTCTTTT
TCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTGA
CAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTC
CTGAGTACAGAAGGCAGGCGGCGCAGCCTCCCCACTGCCCAGCACCCGAG
GGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCCTCCACGTCCGT
CAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCC
ACGCCCTGTGCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAG
CGCGAGCAGGACCCGCGTGTCGCCCCTCAGCAGTGTGCGGTCTGCCTGCA
GCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGCT
GCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGC
GTGCTGAACAACAACAGCTACGAGTCAGACATCCTGAAGAATTACCTGGC
AACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAGCCTCGTGGCTC
TCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACC
GTTCTGTGTTACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCA
GTATCGGCAGAACATTCCTGCTTCCGAGTTGCCAGTGGCCGTAACATCCC
GTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCAC
CACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAACTA
Agcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMERPEEGKQSPPPQPWGRL
LRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKS
GQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYL
YESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQ
VCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSG
PSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLN
GQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQP
CMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDK
SRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESS
DISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTT
AVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCH
LYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGL
TWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQN
IPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent change at R382 (AGA > AGG) compared to NCBI ref
• Low Temp: No