cDNA Clone FOXD2

• Plasmid: pCS2+-DU-HA-FOXD2 full-length
• Parent Plasmid: pCS2+-DU-HA
• Expressed: HA-FOXD2
• Genbank: NM_004474.3 → NP_004465.3 Gene 2306
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCCCCGGGGAATTCATGACCCTGGGCAGCTGCTGCTGCGAGATCAT
GTCCTCCGAGAGCTCCCCGGCCGCGCTGTCCGAGGCCGACGCAGACATAG
ACGTGGTGGGCGGCGGCAGCGGCGGGGGGGAGCTCCCAGCTCGCTCCGGG
CCCCGCGCCCCCCGGGACGTGCTCCCCCACGGCCACGAGCCTCCCGCGGA
GGAAGCCGAGGCAGACTTAGCCGAGGACGAGGAGGAGTCTGGTGGCTGCT
CGGACGGCGAGCCCCGCGCTCTGGCGTCCCGGGGGGCGGCGGCCGCAGCG
GGGAGCCCGGGGCCAGGCGCCGCGGCGGCCCGCGGCGCAGCGGGGCCCGG
GCCGGGACCGCCGTCGGGGGGCGCGGCGACGCGGAGCCCGCTGGTGAAGC
CGCCCTACTCGTACATCGCGCTCATCACCATGGCCATCCTGCAGAGCCCC
AAGAAGCGGCTGACGTTGAGCGAGATCTGCGAGTTCATCAGCGGCCGCTT
CCCCTACTACCGGGAGAAGTTCCCCGCCTGGCAGAACAGCATCCGCCACA
ACCTCTCTCTCAACGACTGCTTCGTCAAGATCCCCCGCGAGCCGGGCAAC
CCGGGCAAGGGCAACTACTGGACGCTGGACCCGGAGTCGGCCGACATGTT
CGACAACGGCAGCTTCCTGCGGCGTCGCAAGCGCTTCAAGCGGCAGCCCC
TGCCGCCGCCGCACCCACACCCGCACCCTCACCCGGAGCTGCTGCTGCGT
GGCGGGGCCGCGGCGGCGGGGGATCC

Amino Acid Sequence

MYPYDVPDYAGSPGEFMTLGSCCCEIMSSESSPAALSEADADIDVVGGGS
GGGELPARSGPRAPRDVLPHGHEPPAEEAEADLAEDEEESGGCSDGEPRA
LASRGAAAAAGSPGPGAAAARGAAGPGPGPPSGGAATRSPLVKPPYSYIA
LITMAILQSPKKRLTLSEICEFISGRFPYYREKFPAWQNSIRHNLSLNDC
FVKIPREPGNPGKGNYWTLDPESADMFDNGSFLRRRKRFKRQPLPPPHPH
PHPHPELLLRGGAAAAGDPGAFLPGFAAYGAYGYGYGLALPAYGAPPPGP
APHPHPHPHPHAFAFAAAAAAAPCQLSVPPGRAAAPPPGPPTASVFAGAG
SAPAPAPASGSGPGPGPAGLPAFLGAELGCAKAFYPASLSPPAAGTAAGL
PTALLRQGLKTDAGGGAGGGGAGAGQRPSFSIDHIMGHGGGGAAPPGAGE
GSPGPPFAAAAGPGGQAQVLAMLTAPALAPVAGHIRLSHPGDALLSSGSR
FASKVAGLSGCHF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29323
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FOXD2 initiating Met is M17 of fusion protein; Clone contains Ala368Pro listed SNP. Clone also contains an extra PH repeat immediately prior to Ala294 compared to NCBI ref; Parental 28162. Synthetic fragment comprising first 750bp ligated Bam-HI into precursor 29214.
• Low Temp: No