cDNA Clone IKKA

• Plasmid: pSC-B IKKa
• Parent Plasmid: pSC-B
• Genbank: NM_001278.4
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC
CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGTCTGTC
TGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCGC
CTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGAT
TATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCTG
AAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATAC
TGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTG
TGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTG
GGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAAAACCT
GAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAAT
TGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTT
TTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCT
TACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATG
TATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGC
ATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAG
ATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAATAGCCT
TTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTGA
ATTGGGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAG
CCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACA
CATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACCACCTG
ATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATA
AATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCC
TCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGATA
GCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCA
TTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAG
CAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGAAGCAG
TGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTCAGGGA
CAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAA
AATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGG
AGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAG
ATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAAT
GGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGG
AGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGAAGAGC
CCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAGCGTGC
CATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACA
GTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAG
GACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTG
TAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGTA
ATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAAGGCAG
AAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCTGCCCG
GTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCAG
CATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTG
GTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTT
GAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGAGGAAC
AGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAATGAGCG
GCCGC

Amino Acid Sequence

MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLEL
STKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSG
GDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENI
VLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTA
TVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSG
EVRFSSHLPQPNSLCSLIVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRC
FVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTG
SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFAS
RSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTY
GISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYG
RRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRV
LKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEI
WHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTP
QDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Trp282 restored according to NCBI NM_001278.4 update Dec 2017; clone harbours g802>a (Val268Ile) listed SNP plus two silent mutations:; a1932>g (silent at G644); c1971>a (silent at A657)
• Low Temp: No