cDNA Clone ISG15

• Plasmid: pSc-B-ISG15 (aa1-154) C78S T3C intein overlap
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

MGCSSGGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRL
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDAL

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29472
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG inserted after Met1 and 3' end overlaps intein (to Spe1 site) and includes T3C mutation. No stop. For cloning into Nhe1 Spe1 sites of pTXB1
• Low Temp: No