cDNA Clone GFP s1-10

• Plasmid: pbabe D puro-saGFP s1-10 (Human optimised)
• Parent Plasmid: pbabe D puro
• Expressed: GFP strands 1-10

Sequence of Insert

ggatccATGAGCAAGGGCGAAGAACTGTTCACCGGCGTGGTGCCCATTCT
GGTGGAACTGGACGGGGATGTGAACGGCCACAAGTTCAGCGTTAGAGGCG
AAGGCGAAGGGGATGCCACAATCGGCAAGCTGACCCTGAAGTTCATCTGC
ACCACCGGAAAGCTGCCCGTGCCTTGGCCTACACTGGTCACCACACTGAC
ATACGGCGTGCAGTGCTTCAGCAGATACCCCGACCATATGAAGCGGCACG
ACTTCTTCAAGAGCGCCATGCCTGAGGGCTACGTGCAAGAGAGAACCATC
AGCTTCAAGGACGACGGCAAGTACAAGACCAGAGCCGTGGTCAAGTTCGA
GGGCGACACCCTGGTCAACCGGATCGAGCTGAAGGGCACCGATTTCAAAG
AGGACGGCAACATCCTGGGCCACAAGCTCGAGTACAACTTCAACAGCCAC
AACGTGTACATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTT
CACCGTGCGGCACAACGTGGAAGATGGCTCTGTGCAGCTGGCCGACCACT
ACCAGCAGAACACCCCTATCGGAGATGGCCCAGTGCTGCTGCCCGACAAT
CACTACCTGAGCACCCAGACCGTGCTGAGCAAGGACCCTAACGAGAAGGG
CACATGAgcggccgc

Amino Acid Sequence

MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTT
GKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISF
KDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNV
YITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHY
LSTQTVLSKDPNEKGT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29480
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: human codon optimized version of the s1-10 fragment of GFP in 29201; Parental 33769
• Low Temp: No