cDNA Clone ISG15

• Plasmid: pTXB1-ISG15 (aa1-154) C78S T3C Mxe intein CBD
• Parent Plasmid: pTXB1
• Expressed: ISG15 (aa1-154) C78S T3C Mxe intein CBD
• Genbank: NM_005101.4
• Tag: Intein
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCGGCTGGGACCTGACGGTGAAGATGCT
GGCGGGCAACGAATTCCAGGTGTCCCTGAGCAGCTCCATGTCGGTGTCAG
AGCTGAAGGCGCAGATCACCCAGAAGATCGGCGTGCACGCCTTCCAGCAG
CGTCTGGCTGTCCACCCGAGCGGTGTGGCGCTGCAGGACAGGGTCCCCCT
TGCCAGCCAGGGCCTGGGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACA
AATCCGACGAACCTCTGAACATCCTGGTGAGGAATAACAAGGGCCGCAGC
AGCACCTACGAGGTGCGGCTGACGCAGACCGTGGCCCACCTGAAGCAGCA
AGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCG
AGGGGAAGCCCCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAG
CCCCTGAGCACCGTGTTCATGAATCTGCGCCTGTGCATCTGCGGAGATGC
ACTAGT

Amino Acid Sequence

MGCSSGGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRL
AVHPSGVALQDRVPLASQGLGPGSTVLLVVDKSDEPLNILVRNNKGRSST
YEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPL
STVFMNLRLCICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRH
GNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLW
KLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLE
AHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFIT
NGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQP
HTSLAGWEPSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pTXB1-ISG15 (aa1-154) C78S T3C Mxe intein CBD90.72 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU29486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: additional N-terminal residues (MAS) derived from translation initiation at the pTXB1 vector derived Nde1 site. Native ISG15 Met1 = Met4 of translation product (with GCSSG inserted between native Met1 and Gly2); Intein T3C mutant; Carries known SNP S83N and silent change at Val98 (GTA>GTG) compared to NCBI ref NM_005101.4; PARENTAL 45876
• Low Temp: No