cDNA Clone CTNNB1

• Synonyms: beta catenin
• Plasmid: pBabeD puro FLAG CTNNB1 isoform 1
• Parent Plasmid: pBabeD puro FLAG
• Expressed: FLAG-CTNNB1 isoform 1
• Genbank: NM_001098209.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGCTACTCAAGCTGATTTGATGGAGTTGGACATGGCCATGGA
ACCAGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGG
ACTCTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGT
AAAGGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGA
GTGGGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGATA
TTGATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATG
TTCCCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGA
TGCTGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGC
TGAAACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTT
GCCACACGTGCAATCCCTGAACTGACAAAACTGCTAAATGACGAGGACCA
GGTGGTGGTTAATAAGGCTGCAGTTATGGTCCATCAGCTTTCTAAAAAGG
AAGCTTCCAGACACGCTATCATGCGTTCTCCTCAGATGGTGTCTGCTATT
GTACGTACCATGCAGAATACAAATGATGTAGAAACAGCTCGTTGTACCGC
TGGGACCTTGCATAACCTTTCCCATCATCGTGAGGGCTTACTGGCCATCT
TTAAGTCTGGAGGCATTCCTGCCCTGGTGAAAATGCTTGGTTCACCAGTG
GATTCTGTGTTGTTTTATGCCATTACAACTCTCCACAACCTTTTATTACA
TCAAGAAGGAGCTAAAATGGCAGTGCGTTTAGCTGGTGGGCTGCAGAAAA
TGGTTGCCTTGCTCAACAAAACAAATGTTAAATTCTTGGCTATTACGACA
GACTGCCTTCAAATTTTAGCTTATGGCAACCAAGAAAGCAAGCTCATCAT
ACTGGCTAGTGGTGGACCCCAAGCTTTAGTAAATATAATGAGGACCTATA
CTTACGAAAAACTACTGTGGACCACAAGCAGAGTGCTGAAGGTGCTATCT
GTCTGCTCTAGTAATAAGCCGGCTATTGTAGAAGCTGGTGGAATGCAAGC
TTTAGGACTTCACCTGACAGATCCAAGTCAACGTCTTGTTCAGAACTGTC
TTTGGACTCTCAGGAATCTTTCAGATGCTGCAACTAAACAGGAAGGGATG
GAAGGTCTCCTTGGGACTCTTGTTCAGCTTCTGGGTTCAGATGATATAAA
TGTGGTCACCTGTGCAGCTGGAATTCTTTCTAACCTCACTTGCAATAATT
ATAAGAACAAGATGATGGTCTGCCAAGTGGGTGGTATAGAGGCTCTTGTG
CGTACTGTCCTTCGGGCTGGTGACAGGGAAGACATCACTGAGCCTGCCAT
CTGTGCTCTTCGTCATCTGACCAGCCGACACCAAGAAGCAGAGATGGCCC
AGAATGCAGTTCGCCTTCACTATGGACTACCAGTTGTGGTTAAGCTCTTA
CACCCACCATCCCACTGGCCTCTGATAAAGGCTACTGTTGGATTGATTCG
AAATCTTGCCCTTTGTCCCGCAAATCATGCACCTTTGCGTGAGCAGGGTG
CCATTCCACGACTAGTTCAGTTGCTTGTTCGTGCACATCAGGATACCCAG
CGCCGTACGTCCATGGGTGGGACACAGCAGCAATTTGTGGAGGGGGTCCG
CATGGAAGAAATAGTTGAAGGTTGTACCGGAGCCCTTCACATCCTAGCTC
GGGATGTTCACAACCGAATTGTTATCAGAGGACTAAATACCATTCCATTG
TTTGTGCAGCTGCTTTATTCTCCCATTGAAAACATCCAAAGAGTAGCTGC
AGGGGTCCTCTGTGAACTTGCTCAGGACAAGGAAGCTGCAGAAGCTATTG
AAGCTGAGGGAGCCACAGCTCCTCTGACAGAGTTACTTCACTCTAGGAAT
GAAGGTGTGGCGACATATGCAGCTGCTGTTTTGTTCCGAATGTCTGAGGA
CAAGCCACAAGATTACAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTC
TCTTCAGAACAGAGCCAATGGCTTGGAATGAGACTGCTGATCTTGGACTT
GATATTGGTGCCCAGGGAGAACCCCTTGGATATCGCCAGGATGATCCTAG
CTATCGTTCTTTTCACTCTGGTGGATATGGCCAGGATGCCTTGGGTATGG
ACCCCATGATGGAACATGAGATGGGTGGCCACCACCCTGGTGCTGACTAT
CCAGTTGATGGGCTGCCAGATCTGGGGCATGCCCAGGACCTCATGGATGG
GCTGCCTCCAGGTGACAGCAATCAGCTGGCCTGGTTTGATACTGACCTGT
AAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSMATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGA
TTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMT
RAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNL
INYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIM
RSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPA
LVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKT
NVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWT
TSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLS
DAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVC
QVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHY
GLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQL
LVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIV
IRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAP
LTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMA
WNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEM
GGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29691
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 1; Silent change at T214 (ACC > ACT) ; Parental 37983
• Low Temp: No