cDNA Clone DENND4C (mouse)

• Plasmid: pCMV5 Flag DENND4C Mouse
• Parent Plasmid: pCMV5
• Expressed: Flag DENND4C Mouse
• Genbank: A6H8H2
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GATAGAAGACAAAGGACCTAGAGTGACAGACTACTTCGTTGTGGCAGGTCTCACTGATACATCCACTCTTTTGGATCAAGAAATAAATCGTACAGATACTAACTCGATTGGTCCTAAAGCTCCAATCACAGACATTGCTGTTATTATCAAATCAGCTGGGGAAACTGTACCTGAAGGCTACACCTGTGTGGAAGCCACTCCATCAGCACTCCAAGCAAACTTGAATTATGGAAGTCTGAAGAGCCCAGAGCTTTTCCTGTGCTACAGGAGAGGGAGAGATAAGCCGCCCCTTACAGATATTGGCGTGCTATATGAAGGGAAAGAAAGGCTTATGCCAGGATGTGAAGTGATCCAAGCCACGCCGTACGGCCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCTCAAAGAATCTTTATCACCTATCGAAGGGCTCCCCCAGTGCGGTCCCAGAATTCCTTGGCCGTCACTGATATCTGTGTTATTATAACCAGTAAAGGAGAAACCCCTCCGCATACTTTCTGCAAAGTGGATAAAAATTTAAACTGTGGCATGTGGGGTTCCAACGTGTTTCTGTGTTACAAGAAGTCTGTGCCTGCTTCAAATGCAATAGCATATAAGGCTGGTTTGATTTTTCGATATCCGGAAGAAGACTATGAGTCTTTTCCGCTCTCACCATCTGTGCCTCTCTTCTGCCTTCCTATGGGAGCCACTATTGAATGCTGGGATCCTCAAATCAAATACCCACTTCCTGTTTTCTCAACATTTGTCCTGACAGGGTCTTCAGCTGAAAAGGTATATGGAGCTGCCATCCAGTTTTATGAACCTTACTCTCAAGAACGTCTAACAGAGAAACAACTTACACAGCTGGGCCTATTGACCCTCGTGGAGAAAAGAGTGGTCTCCAAACCCATCAATTCAAACAAGTGCATTTGTTTGCTTTCACACTGGCCTTTTTTTGAAGCTTTTAAGAATTTTCTTATGTTTATCTACAAAGTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTATGCAAAATATCCCTTTTCCTTCACCACAAAGACCAAGAATCCTCATCCAGCTATCAGTCCATGATGCATTTATATTATCACAGCCAGTGTCCACACCTTTACCATTAAGTGGCGCCAACTTTAGCTCCTTGCTGATGAATCTGGGTCCTGAGAACTGCGCGACATTGCTGCTGCTGGTTTTGCTCGAGAGTAAGATTCTGCTGCACTCTCTGAGGCCAGCGGTACTAACTGGGGTAGCCGAAGCTGTCGTTGCTATGATCTTTCCATTTCAGTGGCAGTGCCCATATATCCCCCTCTGTCCTCTTTCATTGGCTGGAGTGCTTAGTGCGCCTTTACCATTTATAGTTGGAGTGGACTCAAGGTACTTTGATCTTCATGACCCACCACAAGATGTTGTCTGCATTGACTTGGATACGAACACATTATATGTAGCTGATGAAAGAAAGAACATAAACTGGAAGCAGCTTCCCAAAAGGCCGTGCAAGAGTCTGCTTGGCACCCTGAGGAGGCTGTATCAGCAGCTCTGCTCAGTTCACCGAAAACCTCAAGAAAGTTCAGCAATTGAGATGACTCCAATTGAAGCAGATTACTCTTGGCAAAAAAAGATGACACAATTGGAGATGGAAATTCAGGAGACATTTTTGCGATTTATGGCGTCTATTTTAAAAGGGTACAGGTCATATCTCAGACCAATCACAGAAGCTCCTTCTAATAAAGCCACAGCTGCGGATTCATTGTTTGATCGACAGGGTTTTTTGAAAAGTCGGGATCGTGCCTATACAAAATTCTATACTCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAGTTTTGTAAGTGATAAAGATACTGGATTGGCATTTTTTGATGATTGCATCGAGAAGTTGTTTCCAGATAAAGGTGTAGAGAGAACAGAAAAGGTTGACTTGGATTCAGCAGAAGACACCAGATTGATAGAGCTAGATGATTCACAGAGAAGTGAACACACTGTATTTATAATGCCACCTGAGCCGCCTCCTGATGATGGAAATAACTTATCACCTCAGTACAGTTACACATACTTTCCAAGATTGGATCTTAAGCTTTTTGACAGCCCACAGAAGCTGAAACTGTGCTTCAATAGACATCCTCCTGGGAGTAGCATTACAAACAGCCCTGCCCTCATGGCTAAAAGGACTAAACAGGAGATAAAAACAGCTCATAAGTTGGCGAAGAGATGTTATACGAATCCACCACAATGGGCCAAGTATCTATTCAGCCACTGTTACAGTCTGTGGTTTATTTGTCTCCCAGCCTATGTTAGAGTTTCTCACCCTAAGGTCCGAGCACTCCAGCAGGCACATGATGTGCTTGTTAAGATGCGGAAGACAGACGTGGATCCACTAGATGAGGTGTGCTATCGAGTAGTCATGCAGCTTTGTGGACTTTGGGTTAATCCTGTTTTAGCAGTGAGAGTCTTATTCGAGATGAAAACTGCTAGAATAAAACCAAATGCTATCACTTATGGCTATTACAATAAGGTAGTTTTGGAGAGCCCATGGCCCAGCAGTACTCGCAGTGGTATTTTCTTGTGGACGAAAGTACGGAATGTTGTACATGGTTTGGCCCAGTTTAGGCAACCACTTAAAAAGACTGGGCAAAAATCACAGGTCTTTTCAATATCAGGCGGTCAGTCTGACCAGGGCTACGGGTCTAAGGATGAGCTGGTAAAGGAGGGTGCAGATGGTCACGCCCCTGAAGAGCACACGCCACCAGAGCTGACGACCACAGAGCTGCACATCGAAGAGGAGTGTGATATATCTGCCATTGTCTCAAAACACCTGCAGCCTACTCCTGAACCTCAGAGTCCTACGGAGCCTCCTGCATGGGGCAGCAGTATTGTGAAAGTTCCATCTGGGTTATTTGATACCAACAATAGGACAAGTACTGGTAGTACTTCAACTGTGCTATTTTCTACTCAAGCTCCAGTCGAGGATGCAGTCTTCAGTGAGGTTACTAATTTCAAGAAGAATGGTGATAGAGGAGAGAAAAAACAGAAGCATTTCCCTGAGAGGAGCTGTAGCTTTAGTTCTGAAAGTCGAGCGGGGATGCTGCTTAAGAAGAGCAGTTTGGACCTGAATTCAAGCGAAATGGCCATCATGATGGGAGCAGATGCCAAGATCCTCACAGCAGCACTGACATGTCCTAAGACTTCCCCGCCTCATGTCACAAGAACTCACAGCTTTGAGAATGTTAACTGTCATCTAGCTGACAGTAGGACTCGCATGTCAGAAGGCACCCGGGATTCTGAGCACAGATCATCTCCTGTGCTAGAGATGCTGGAGGAAAGCCAGGAGCTCCTGGAGCCTGTGGTTGGTGATAATGTGGCAGAGACTGCGGCAGAGATGACGTGTAACAGTCTACAAAGTAACAGTCACAGTGACCAGTCCAGAGACACACAAGCTGGAGCCCAAGATCCAGTGAACAAGAGGAGCAGTTCATATGCTACTAGGAAAGCCATTGAGAGGGAGGATGTGGAGACGGGACTAGATCCGTTGTCTCTTTTAGCCACTGAATGTGTAGAAAAAACGTCTGATTCTGAAGATAAGTTATTTTCTCCAGTAATTTCACGTAATCTGGCTGATGAAATTGAAAGTTACATGAACCTAAAAAGCCCACTAGGCAGTAAATCTTGTAGTATGGAATTGCATGGAGAGGGGAACCAGGAGCCTGGCTCTCCTGCTGTGTTTGCTCACCCTTTAGAAAGGAGCTCGAGTCTGCCTTCAGACCGTGGCCCGCCAGCACGAGACAGCACCGAGACTGAGAAGAGCTCCCCTGCTGTGTCCAGCTCTAAAACTCTGACTGGGCGTTTCAAGCCACAGAGTCCTTACCGCGCTTACAAAGACCGGTCCACTTCCTTATCAGCATTAGTACGTTCTTCACCAAACAGCTCTCTGGGTTCTGTAGTCAATTCTCTATCGGGGCTAAAGTTGGACAATATACTCTCAGGGCCCAAGATAGATGTCCTAAAATCTAGTATGAAACAAGCAGCAACGGTGGCCAGTAAGATGTGGGTGGCTGTAGCATCTGCCTATAGCTACTCAGATGACGAGGAGGAAACTAATAAAGATTACAGTTTCCCAGCTGGCCTAGAAGACCATCATATAGTGGGAGAGACTCTGTCGCCTAACACAAGTGTCTCAGGGTTGGTTCCCAGTGAACTGACCCAGAGCAACACAAGCCTCGGCAGTAGTAGCAGCAGTGGAGACGTTGGAAAACTGCAGTGTCCAGCAGGTGAAGTTCCATTTTCAAGAAATATCAAAGGGCAGGACTTTGAAAAGTCAGACCATGGTTCTTCTCAGAATACCAGTATGTCTAGCATCTATCAAAATTGTGCGATGGAGGTTTTGATGTCGAGTTGCTCACAGTGCAGAGCTTGTGGCGCTCTGGTTTATGATGAGGAAATCATGGCTGGATGGACAGCAGATGACTCAAATTTGAATACAACCTGCCCGTTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATGTAGAATTTAAAGACCTGCGAGGCTCTGCCAGCTTTTTCTTGAAACCAAGCACGTCTGGTGACAGTTTACAAAGCGGCAGCATTCCATCAGCCAGTGAGCCCTCTGAACACAAACCCACGTCCAGTTCAGCAGAACCTGACCTGATCAGCTTTATGGATTTCTCAAAACACAGTGAGACCATAACAGAAGAAGCAAGTTATACAGTCGAATCAAGTGATGAAATAAAGAAAACCAATGGTGATGTCCAGAGTGTGAAAATGTCATCTGTGCCTAACAGTTTATCTAAGCGAAACGTATCTTTGACTCGAAGTCACAGTGTTGGAGGCCCTCTGCAGAATATCGACTTCTCCCAGCGACCATTTCATGGCGTCTCAACAGTCAGTCTTCCAAGCAGTCTGCAGGAAGATGTGGACCATCTAGGCAAGCGACCCAGTCCTCCTCCTGTGTCTGTGCCCTATTTGAGTCCTCTAGTGCTTCGGAAGGAACTTGAATCTTTGCTAGAAAATGAAGGTGATCAGGTCATCCATACATCCTCATTCATCAATCAACACCCAATCATTTTCTGGAACCTGGTTTGGTATTTCAGACGTTTGGACCTTCCTAGCAACTTGCCAGGCCTTATTCTCACCTCTGAGCATTGTAATGGAGGTGTGCAGCTTCCTCTATCATCTTTGTCCCAGGACAGCAAACTGGTGTATATTCAACTGCTGTGGGATAATATCAACCTTCATCAGGAACCAGGAGAACCTCTGTATGTCTCTTGGAGGAACTTGAATTCTGAGAAGAAGCCGTCACTTCTGTCAGAGCAGCAGCAGGCAGCCAGCGCTTTAGTGGAGACGATCAGGCAGAGCATCCAGCAGAATGACGTTCTCAAGCCCATCAACCTGCTTTCCCAGCAAATGAAGCCAGGCACGAAACGGCAGAGGAGTTTATACAGAGAAATCCTTTTCTTATCATTAGTGTCTCTTGGGAGAGAAAACATTGATATTGAGGCTTTTGACAATGAATATGGACTCGCATACAGAAGTCTGCCTTCAGAGAGTCTTGAAAGGTTACAGAGAATCGATGCCCCACCCAGTATCAGTGTGGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATTTAAGTCGAC

Amino Acid Sequence

IEDKGPRVTDYFVVAGLTDTSTLLDQEINRTDTNSIGPKAPITDIAVIIKSAGETVPEGYTCVEATPSALQANLNYGSLKSPELFLCYRRGRDKPPLTDIGVLYEGKERLMPGCEVIQATPYGRCANVNNSSTTSQRIFITYRRAPPVRSQNSLAVTDICVIITSKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNAIAYKAGLIFRYPEEDYESFPLSPSVPLFCLPMGATIECWDPQIKYPLPVFSTFVLTGSSAEKVYGAAIQFYEPYSQERLTEKQLTQLGLLTLVEKRVVSKPINSNKCICLLSHWPFFEAFKNFLMFIYKVSVSGPHPLPIEKHISHFMQNIPFPSPQRPRILIQLSVHDAFILSQPVSTPLPLSGANFSSLLMNLGPENCATLLLLVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNTLYVADERKNINWKQLPKRPCKSLLGTLRRLYQQLCSVHRKPQESSAIEMTPIEADYSWQKKMTQLEMEIQETFLRFMASILKGYRSYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYTKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDCIEKLFPDKGVERTEKVDLDSAEDTRLIELDDSQRSEHTVFIMPPEPPPDDGNNLSPQYSYTYFPRLDLKLFDSPQKLKLCFNRHPPGSSITNSPALMAKRTKQEIKTAHKLAKRCYTNPPQWAKYLFSHCYSLWFICLPAYVRVSHPKVRALQQAHDVLVKMRKTDVDPLDEVCYRVVMQLCGLWVNPVLAVRVLFEMKTARIKPNAITYGYYNKVVLESPWPSSTRSGIFLWTKVRNVVHGLAQFRQPLKKTGQKSQVFSISGGQSDQGYGSKDELVKEGADGHAPEEHTPPELTTTELHIEEECDISAIVSKHLQPTPEPQSPTEPPAWGSSIVKVPSGLFDTNNRTSTGSTSTVLFSTQAPVEDAVFSEVTNFKKNGDRGEKKQKHFPERSCSFSSESRAGMLLKKSSLDLNSSEMAIMMGADAKILTAALTCPKTSPPHVTRTHSFENVNCHLADSRTRMSEGTRDSEHRSSPVLEMLEESQELLEPVVGDNVAETAAEMTCNSLQSNSHSDQSRDTQAGAQDPVNKRSSSYATRKAIEREDVETGLDPLSLLATECVEKTSDSEDKLFSPVISRNLADEIESYMNLKSPLGSKSCSMELHGEGNQEPGSPAVFAHPLERSSSLPSDRGPPARDSTETEKSSPAVSSSKTLTGRFKPQSPYRAYKDRSTSLSALVRSSPNSSLGSVVNSLSGLKLDNILSGPKIDVLKSSMKQAATVASKMWVAVASAYSYSDDEEETNKDYSFPAGLEDHHIVGETLSPNTSVSGLVPSELTQSNTSLGSSSSSGDVGKLQCPAGEVPFSRNIKGQDFEKSDHGSSQNTSMSSIYQNCAMEVLMSSCSQCRACGALVYDEEIMAGWTADDSNLNTTCPFCKSNFLPLLNVEFKDLRGSASFFLKPSTSGDSLQSGSIPSASEPSEHKPTSSSAEPDLISFMDFSKHSETITEEASYTVESSDEIKKTNGDVQSVKMSSVPNSLSKRNVSLTRSHSVGGPLQNIDFSQRPFHGVSTVSLPSSLQEDVDHLGKRPSPPPVSVPYLSPLVLRKELESLLENEGDQVIHTSSFINQHPIIFWNLVWYFRRLDLPSNLPGLILTSEHCNGGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYVSWRNLNSEKKPSLLSEQQQAASALVETIRQSIQQNDVLKPINLLSQQMKPGTKRQRSLYREILFLSLVSLGRENIDIEAFDNEYGLAYRSLPSESLERLQRIDAPPSISVEWCRKCFGAPLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU62450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has no Methionine and is waiting for further sequencing to confirm 3Flag tag at 5' end
• Low Temp: No