cDNA Clone MYCBP2

• Plasmid: pcDNA5-FRT/TO-MYC-MYCBP2 FL precursor (+Xho1)
• Parent Plasmid: pcDNA5-FRT/TO-Myc
• Tag: Myc
• Species: Human

Sequence of Insert

GCTAGCATGCCGGTTCCCGACGGCTCCGTGGCTGCTGCGGGGCTGGGGCT
GGGGCTACCCGCCGCGGACTCCCGGGGTCACTACCAGCTGCTGCTGTCAG
GCCGGGCCCTGGCCGACCGCTACCGGAGGATTTATACCGCTGCGCTCAAT
GACAGGGACCAGGGGGGCGGCAGCGCTGGACACCCAGCCTCCAGGAATAA
GAAAATTTTAAATAAGAAGAAATTGAAAAGAAAACAGAAGAGCAAATCAA
AAGTGAAGACAAGAAGCAAGTCTGAAAACTTAGAGAATACAGTAATCATA
CCAGATATCAAACTACATAGCAATCCTTCTGCTTTCAATATTTACTGTAA
TGTACGCCATTGCGTTCTGGAATGGCAGAAGAAGGAAATATCATTGGCAG
CCGCATCTAAGAACTCTGTGCAGAGTGGAGAATCAGATAGTGATGAAGAA
GAGGAATCCAAAGAGCCCCCTATCAAGCTTCCAAAGATTATTGAGGTTGG
CCTTTGTGAAGTTTTTGAATTGATCAAAGAGACACGATTTTCTCATCCAT
CCCTGTGTCTCAGGAGTCTCCAAGCCCTGCTCAACGTGCTGCAGGGCCAG
CAGCCAGAAGGCCTCCAGTCTGAGCCACCTGAGGTCCTAGAGTCTCTCTT
CCAGCTTCTTTTGGAAATCACCGTTCGAAGTACTGGGATGAATGACAGCA
CAGGACAGTCCTTAACAGCACTTTCCTGTGCTTGCCTCTTTAGTCTGGTG
GCTTCTTGGGGAGAAACAGGAAGGACACTTCAGGCCATCTCTGCTATCCT
CACCAACAATGGAAGCCATGCTTGCCAAACTATTCAGGTGCCAACAATTC
TAAATTCGCTACAGAGAAGTGTACAAGCAGTTTTGGTGGGAAAAATTCAA
ATTCAGGACTGGTTTAGTAATGGCATTAAGAAAGCAGCTTTAATGCACAA
GTGGCCATTAAAAGAAATATCTGTTGATGAAGATGACCAATGTCTACTTC
AGAATGATGGATTTTTTCTTTATCTATTATGCAAGGATGGATTATATAAA
ATAGGCTCTGGATACAGTGGAACAGTTAGGGGCCATATATACAATTCTAC
ATCCCGTATTAGAAACAGAAAAGAAAAAAAGTCTTGGTTAGGGTATGCTC
AGGGTTATTTATTATATAGAGATGTGAATAACCACAGCATGACAGCCATA
AGGATAAGCCCTGAAACACTGGAGCAAGATGGTACTGTGATGTTACCAGA
TTGCCACACTGAAGGTCAAAATATTTTATTCACTGATGGAGAATATATTA
ATCAGATAGCTGCTTCAAGAGATGATGGCTTTGTTGTCAGAATATTTGCC
ACAAGCACTGAACCTGTTCTACAGCAAGAATTGCAACTTAAACTGGCTAG
AAAATGCTTACATGCCTGTGGTATCTCACTATTCGATCTGGAAAAGGACT
TGCATATTATAAGTACAGGATTTGATGAGGAGTCAGCAATTCTTGGTGCA
GGACGAGAGTTTGCGCTAATGAAAACAGCAAATGGAAAGATATATTACAC
TGGCAAATACCAGAGTCTTGGAATCAAACAAGGTGGTCCTTCAGCAGGAA
AATGGGTTGAGCTACCAATTACAAAATCTCCAAAGATAGTACACTTCTCA
GTTGGACACGATGGCTCTCACGCCCTTTTAGTTGCAGAAGATGGGAGCAT
ATTCTTTACAGGATCTGCTAGTAAAGGAGAAGATGGAGAATCAACTAAGA
GCAGACGGCAATCCAAACCTTATAAACCTAAAAAGATAATTAAGATGGAA
GGAAAGATTGTGGTATATACAGCCTGCAATAATGGAAGTAGTTCTGTTAT
TTCTAAAGATGGAGAACTCTACATGTTTGGAAAAGATGCCATTTACTCTG
ATAGTTCAAGTTTGGTAACTGATTTGAAGGGCCATTTTGTAACTCAGGTA
GCTATGGGCAAAGCTCACACTTGTGTTTTAATGAAGAATGGAGAGGTGTG
GACATTTGGTGTAAATAATAAAGGACAGTGTGGACGAGATACTGGTGCCA
TGAACCAAGGTGGGAAAGGGTTTGGAGTTGAAAATATGGCAACAGCAATG
GATGAAGACCTGGAAGAAGAACTAGATGAAAAAGATGAGAAGTCTATGAT
GTGCCCTCCAGGCATGCACAAATGGAAGCTGGAGCAGTGCATGGTTTGCA
CTGTCTGTGGAGACTGTACAGGTTATGGAGCCAGCTGTGTCAGTAGTGGA
CGGCCAGACAGAGTCCCCGGAGGGATCTGTGGTTGTGGTTCCGGAGAATC
TGGTTGTGCTGTGTGTGGATGTTGCAAGGCCTGTGCAAGAGAGTTAGATG
GTCAAGAGGCAAGACAAAGAGGAATTCTTGATGCAGTGAAAGAAATGATA
CCTTTAGATCTTCTTTTAGCTGTCCCAGTGCCCGGGGTTAACATTGAAGA
ACACCTTCAGTTACGACAAGAAGAAAAACGGCAACGTGTAATCAGAAGGC
ACAGATTAGAGGAAGGAAGAGGCCCCCTTGTATTTGCTGGTCCTATTTTT
ATGAACCATCGAGAACAGGCTCTAGCCAGACTCAGATCCCATCCAGCACA
GCTAAAGCATAAACGGGACAAGCACAAAGATGGAAGTGGAGAAAGAGGCG
AAAAGGATGCAAGCAAAATCACAACATACCCTCCAGGCTCTGTGCGATTT
GACTGTGAGCTCCGGGCAGTCCAAGTCAGCTGTGGATTTCACCATTCAGT
GGTTTTAATGGAAAATGGAGATGTCTATACATTTGGTTATGGGCAGCATG
GGCAGCTAGGACATGGAGATGTCAACTCCAGGGGATGTCCCACTCTTGTT
CAAGCATTGCCAGGCCCTAGCACACAAGTCACTGCAGGCAGCAACCATAC
GGCAGTACTTTTAATGGATGGACAGGTCTTCACATTTGGAAGTTTTTCTA
AAGGACAACTGGGCAGACCAATTTTGGATGTGCCATATTGGAATGCAAAG
CCAGCTCCCATGCCTAACATTGGATCAAAATATGGAAGAAAAGCTACTTG
GATAGGTGCAAGTGGGGACCAAACTTTTTTACGAATTGATGAAGCACTTA
TTAATTCTCATGTACTTGCTACATCAGAAATTTTTGCCAGTAAACACATA
ATAGGCTTGGTACCTGCTTCTATATCAGAACCTCCTCCATTTAAATGCCT
TCTGATAAATAAAGTGGATGGGAGTTGTAAAACTTTTAATGACTCAGAAC
AAGAGGATCTGCAAGGATTTGGTGTGTGTCTTGATCCTGTATACGATGTA
ATTTGGAGGTTTCGACCAAATACTAGAGAGCTGTGGTGTTACAATGCGGT
GGTTGCTGATGCCAGGCTTCCCTCTGCAGCAGACATGCAGTCCAGATGTA
GTATCCTAAGTCCTGAACTTGCCTTACCAACAGGATCAAGGGCCCTCACT
ACCCGATCTCATGCAGCTTTGCACATTTTAGGTTGTCTTGATACCTTGGC
AGCTATGCAGGACTTAAAAATGGGTGTTGCAAGTACAGAGGAAGAGACTC
AAGCAGTAATGAAGGTTTATTCTAAAGAAGATTATAGTGTGGTAAACAGG
TTTGAAAGTCATGGAGGAGGCTGGGGTTATTCTGCCCATTCAGTAGAAGC
TATACGTTTCAGTGCCGACACTGATATTTTACTTGGTGGTCTTGGTCTGT
TTGGAGGTAGAGGAGAATATACTGCTAAAATTAAGCTGTTTGAATTGGGT
CCTGATGGAGGAGATCATGAAACTGATGGTGACCTTCTTGCAGAGACTGA
TGTATTGGCTTATGACTGTGCTGCTAGAGAAAAATATGCAATGATGTTTG
ATGAGCCTGTTCTCCTGCAAGCTGGGTGGTGGTATGTGGCATGGGCCCGA
GTGTCAGGACCCAGCAGTGACTGTGGATCTCATGGACAGGCATCTATTAC
CACAGATGATGGGGTTGTTTTCCAGTTCAAGAGTTCAAAGAAATCAAATA
ATGGTACAGATGTTAATGCGGGTCAGATACCTCAGTTATTATACAGACTT
CCAACCAGTGATGGCAGTGCTTCAAAAGGCAAACAGCAAACCAGTGAACC
TGTACACATTTTAAAGAGGTCTTTTGCAAGAACTGTCTCAGTGGAATGTT
TTGAGTCATTGTTGAGTATTCTTCACTGGAGCTGGACCACCTTAGTCTTA
GGAGTTGAAGAACTTAGAGGATTAAAAGGATTCCAGTTCACAGCTACACT
CCTAGATTTAGAGAGACTGCGCTTTGTGGGTACCTGTTGTCTGAGGTTAT
TGCGTGTCTATACCTGTGAAATTTACCCAGTGTCAGCTACAGGAAAAGCA
GTTGTAGAAGAAACTAGCAAATTAGCAGAGTGTATTGGAAAAACCAGAAC
TTTGTTAAGAAAAATTTTATCAGAAGGAGTTGATCACTGCATGGTGAAAT
TGGATAATGATCCTCAAGGATATCTCAGTCAACCCTTGAGTCTTCTAGAA
GCTGTCCTTCAGGAATGTCATAATACTTTCACTGCCTGCTTTCATTCTTT
CTACCCAACTCCTGCCTTACAGTGGGCTTGCCTTTGTGATCTGCTGAATT
GTTTGGATCAGGATATCCAAGAAGCAAACTTCAAGACATCAAGTAGCCGA
CTCCTTGCAGCTGTTATGTCAGCTCTGTGTCACACGTCTGTTAAGCTGAC
TTCCATCTTCCCGATTGCGTATGATGGAGAAGTATTACTACGATCAATTG
TTAAACAAGTTAGTACAGAGAACGACTCAACACTAGTTCATCGTTTTCCC
CTTTTGGTGGCACATATGGAAAAACTCAGCCAGAGTGAAGAGAATATCTC
AGGGATGACAAGCTTCCGTGAAGTTCTGGAGAAAATGCTGGTCATTGTTG
TGCTACCAGTCAGGAACAGCCTGAGGAGAGAAAATGAACTCTTCTCCTCC
CACCTCGTCTCTAACACCTGTGGATTACTGGCCAGCATTGTCAGTGAACT
GACAGTGTCAGCCCTGGGATCTGAGGTTGATGGACTTAATTCTCTTCACT
CTGTAAAAGCTAGTGCTAACCGATTTACAAAAACAAGTCAGGGCAGAAGT
TGGAACACTGGGAACGGGTCCCCTGATGCAATCTGTTTTTCAGTAGACAA
ACCTGGAATAGTTGTGGTTGGTTTCTCTGTCTATGGAGGAGGTGGAATTC
ATGAATATGAATTAGAGGTGTTAGTTGATGATAGTGAACATGCAGGAGAT
TCAACTCATTCCCACAGATGGACATCTCTGGAATTAGTGAAAGGAACGTA
CACAACGGATGACTCACCCAGTGATATAGCTGAGATCAGACTTGACAAAG
TGGTTCCTTTAAAGGAAAATGTTAAATATGCTGTGCGCTTGAGGAACTAT
GGAAGCCGTACAGCCAATGGAGATGGAGGAATGACCACAGTTCAGTGCCC
TGATGGTGTGACATTCACATTCAGCACGTGCAGCTTGAGCAGTAACGGCA
CAAACCAAACCAGAGGACAGATCCCACAGATACTCTACTATAGGAGTGAA
TTTGATGGAGATTTACAATCCCAACTTCTGAGTAAAGCCAATGAAGAAGA
TAAAAACTGTAGCAGAGCATTGTCTGTTGTAAGCACTGTCGTTCGAGCCT
CTAAGGACCTCCTGCACAGAGCTCTTGCTGTGGATGCTGATGACATTCCA
GAACTGCTGAGTTCTTCCAGTCTGTTTTCCATGCTGCTCCCCCTTATTAT
AGCCTACATAGGACCAGTAGCTGCTGCTATTCCCAAGGTGGCTGTAGAAG
TCTTTGGCCTTGTCCAACAATTGCTTCCGTCAGTTGCCATTTTGAATCAG
AAGTATGCACCGCCTGCCTTCAACCCTAATCAGTCGACAGATAGCACCAC
AGGAAACCAGCCTGAACAGGGCCTCTCTGCTTGTACAACCTCCAGTCACT
ATGCTGTCATAGAGAGTGAGCACCCGTATAAACCTGCCTGTGTGATGCAT
TACAAGGTGACATTCCCAGAATGCGTGAGGTGGATGACAATCGAATTTGA
CCCTCAGTGTGGTACTGCACAGTCAGAAGATGTCCTTCGTTTGTTGATTC
CTGTCAGAACTGTTCAGAATTCAGGATATGGACCAAAATTGACATCTGTT
CATGAAAATCTTAATTCATGGATAGAATTAAAGAAATTTTCAGGATCCTC
TGGGTGGCCTACTATGGTTTTGGTGTTGCCAGGAAATGAGGCCCTTTTTT
CATTGGAGACTGCATCAGATTATGTGAAAGATGACAAAGCTTCTTTCTAT
GGTTTTAAGTGTTTTGCAATTGGATATGAATTTAGCCCTGGACCTGATGA
GGGAGTCATCCAATTGGAAAAAGAATTAGCCAATCTTGGTGGGGTTTGTG
CAGCAGCTCTGATGAAGAAGGACCTAGCACTTCCTATTGGTAATGAATTA
GAAGAAGACCTTGAAATTCTTGAGGAGGCTGCATTGCAGGTGTGCAAAAC
CCATTCTGGAATTCTTGGAAAGGGTCTAGCTCTTTCTCATTCACCAACTA
TATTAGAAGCACTTGAGGGAAATTTACCACTCCAAATCCAAAGCAATGAA
CAGTCTTTTCTGGATGATTTTATTGCCTGTGTCCCAGGATCAAGTGGTGG
AAGGCTTGCAAGGTGGCTTCAGCCAGATTCATATGCGGATCCTCAGAAAA
CATCTTTGATCCTGAATAAGGATGATATTCGTTGTGGTTGGCCTACCACC
ATAACTGTTCAAACAAAAGACCAGTATGGGGATGTGGTACATGTTCCCAA
TATGAAGGTGGAAGTGAAAGCTGTCCCTGTTTCTCAGAAAAAAATGTCTT
TACAACAAGATCAAGCAAAGAAACCTCAAAGGATTCCTGGCAGTCCTGCA
GTAACAGCTGCATCTTCTAATACTGACATGACTTATGGAGGGCTGGCATC
ACCAAAGCTAGATGTTTCATATGAACCAATGATAGTGAAGGAAGCTCGAT
ATATTGCCATAACAATGATGAAGCTCGAGGTTTATGAAAATTATTCATTT
GAAGAACTACGTTTTGCATCACCAACTCCTAAGAGACCCAGTGAGAATAT
GCTGATCCGTGTCAATAATGATGGGACTTATTGTGCAAATTGGACTCCAG
GGGCTATTGGACTCTACACTCTTCATGTTACCATTGATGGCATTGAAATC
GATGCTGGTCTGGAAGTAAAAGTAAAAGACCCACCAAAAGGGATGATACC
ACCAGGAACTCAGTTGGTCAAACCAAAGTCTGAACCTCAGCCTAATAAGG
TTCGAAAATTTGTGGCCAAGGACAGTGCGGGGCTTCGCATCCGTAGCCAC
CCTTCCCTTCAGAGTGAGCAGATAGGCATAGTGAAAGTCAATGGAACTAT
CACTTTTATTGATGAGATCCATAATGATGATGGTGTGTGGCTGAGGCTGA
ATGATGAGACAATAAAGAAGTATGTCCCTAACATGAATGGTTACACTGAA
GCCTGGTGCCTCTCTTTTAATCAACATCTTGGCAAGAGTCTTCTGGTCCC
TGTTGACGAATCTAAAACTAATACTGATGACTTTTTCAAAGACATAAACT
CCTGCTGCCCACAGGAAGCAACAATGCAAGAACAAGATATGCCATTCTTG
CGAGGAGGGCCAGGCATGTACAAGGTAGTGAAGACGGGACCTTCAGGTCA
CAACATCAGAAGCTGCCCTAACCTTAGAGGTATCCCAATTGGAATGTTAG
TTCTGGGAAACAAAGTCAAAGCAGTGGGAGAGGTAACCAATTCTGAAGGG
ACATGGGTGCAACTGGATCAGAACAGCATGGTAGAGTTCTGTGAGAGTGA
TGAAGGAGAGGCATGGTCCTTAGCTAGAGACAGAGGCGGAAACCAGTACC
TCCGACATGAAGATGAACAAGCTCTTCTGGATCAGAATTCTCAAACTCCT
CCTCCAAGCCCTTTCTCAGTGCAAGCTTTTAATAAAGGGGCAAGTTGCAG
TGCCCAAGGATTTGATTATGGACTCGGAAATAGCAAAGGTGATCGAGGAA
ACATCTCAACATCTTCTAAACCAGCCTCTACATCAGGAAAATCAGAGCTG
TCCTCTAAACACAGCAGATCGCTTAAACCTGATGGACGTATGAGCCGGAC
TACTGCTGATCAGAAGAAGCCAAGGGGCACAGAAAGTTTATCTGCTAGTG
AATCCCTCATCTTAAAATCTGATGCTGCAAAGTTGAGGTCAGATTCCCAC
AGTAGGTCATTATCCCCCAACCATAACACCTTGCAGACATTGAAATCTGA
TGGGAGGATGCCTTCTAGCTCCAGAGCTGAATCCCCAGGACCAGGTTCTC
GGTTGTCGTCTCCTAAGCCAAAGACTCTCCCAGCCAATAGGTCTAGCCCA
TCGGGTGCTAGTTCTCCACGCTCCTCCTCACCACATGATAAAAATCTACC
TCAAAAAAGTACTGCTCCTGTTAAGACAAAGCTTGATCCTCCTCGGGAAC
GTTCTAAATCAGACTCTTACACACTTGATCCAGATACCCTCCGCAAGAAG
AAAATGCCCCTCACAGAACCTTTGAGAGGACGGTCAACGTCACCAAAACC
AAAATCAGTACCAAAGGATTCTACAGATTCCCCTGGATCTGAAAATAGAG
CTCCCTCTCCCCATGTGGTACAGGAAAACCTCCACAGTGAGGTGGTCGAA
GTCTGCACCTCAAGTACTTTAAAAACAAATAGTCTAACAGACAGCACCTG
CGATGACAGCAGTGAATTTAAGAGTGTGGATGAAGGTTCAAATAAAGTTC
ATTTTAGCATTGGAAAAGCACCACTGAAAGATGAACAGGAAATGAGAGCA
TCTCCCAAAATAAGTCGAAAATGTGCTAATAGACACACCAGGCCCAAAAA
AGAAAAATCGAGTTTTCTTTTCAAAGGAGATGGATCCAAGCCTTTAGAGC
CAGCCAAGCAAGCCATGTCTCCTTCTGTGGCCGAATGTGCCAGAGCTGTG
TTTGCTTCCTTCCTCTGGCATGAAGGCATAGTACATGATGCAATGGCTTG
TTCTTCTTTCCTAAAGTTTCATCCTGAACTTTCCAAAGAACATGCTCCTA
TAAGGAGTAGTTTAAATAGCCAACAACCTACAGAGGAAAAAGAAACCAAG
TTAAAAAATAGACATTCATTAGAAATATCATCTGCACTGAATATGTTTAA
TATTGCACCCCATGGACCAGATATATCTAAGATGGGTAGCATCAACAAAA
ACAAGGTATTGTCTATGCTTAAGGAACCACCTCTGCATGAAAAATGTGAG
GATGGGAAAACCGAGACCACTTTTGAAATGTCCATGCATAACACAATGAA
GTCTAAGTCTCCTCTTCCCTTAACTTTACAACATTTAGTGGCTTTTTGGG
AAGACATCTCTTTGGCTACTATCAAAGCTGCTTCCCAGAATATGATTTTT
CCAAGTCCTGGTTCCTGTGCAGTTCTTAAAAAGAAAGAGTGTGAGAAAGA
GAATAAGAAGTCCAAAAAGGAAAAAAAGAAAAAAGAAAAGGCAGAAGTTA
GGCCCAGGGGTAATTTGTTTGGAGAGATGGCCCAGCTGGCAGTAGGAGGA
CCAGAGAAAGATACCATCTGTGAACTGTGTGGGGAGTCACATCCATACCC
GGTGACCTATCACATGAGACAAGCTCACCCAGGTTGTGGCCGATATGCTG
GTGGACAAGGTTACAATAGCATTGGGCATTTTTGTGGAGGATGGGCTGGT
AACTGTGGTGATGGTGGCATAGGAGGAAGCACTTGGTATCTGGTATGTGA
TCGCTGTAGAGAAAAATACCTCCGCGAAAAACAGGCTGCTGCAAGGGAGA
AGGTCAAACAATCTAGGAGAAAGCCAATGCAAGTCAAGACCCCTCGTGCC
TTGCCCACCATGGAAGCTCACCAGGTGATTAAAGCCAATGCACTCTTCCT
GCTGTCCCTGAGCAGTGCAGCAGAACCGAGCATTCTGTGTTACCATCCTG
CAAAGCCATTCCAATCTCAGTTGCCCAGTGTAAAAGAAGGCATTTCTGAG
GATCTTCCTGTGAAAATGCCTTGTCTCTACCTGCAGACATTAGCTAGGCA
TCATCATGAAAATTTTGTGGGCTATCAAGATGACAATCTATTCCAGGATG
AAATGAGATATCTACGTTCAACATCTGTACCTGCCCCGTATATATCAGTA
ACTCCTGATGCAAGTCCTAATGTATTTGAAGAGCCAGAGAGCAATATGAA
GTCTATGCCACCAAGTTTAGAAACCAGTCCCATAACTGATACTGATCTTG
CAAAGAGAACTGTCTTCCAAAGATCATACTCAGTTGTTGCTTCCGAATAT
GATAAACAACACTCCATTTTACCTGCACGAGTTAAAGCTATTCCTAGAAG
AAGAGTTAACAGTGGAGACACTGAAGTTGGTTCTTCCCTTTTGAGACATC
CGTCTCCTGAGCTTTCTCGGCTAATCTCAGCCCACAGCTCTCTTTCTAAA
GGAGAACGAAATTTCCAGTGGCCAGTTTTAGCTTTTGTTATACAACATCA
TGATCTAGAAGGTCTTGAAATAGCAATGAAACAGGCCCTAAGGAAATCTG
CTTGTCGAGTTTTTGCTATGGAGGCTTTCAACTGGCTTCTGTGTAATGTC
ATCCAAACCACTTCTCTCCATGATATTCTGTGGCATTTTGTGGCATCACT
GACTCCTGCACCAGTGGAACCAGAGGAAGAAGAGGATGAAGAAAATAAAA
CAAGCAAAGAAAATTCAGAACAAGAGAAAGATACAAGAGTATGTGAACAT
CCACTCTCAGACATAGTGATTGCCGGGGAAGCTGCTCATCCTTTACCACA
CACCTTTCACCGCTTGCTGCAGACCATCTCAGACCTTATGATGTCTCTCC
CCAGCGGCAGTTCATTACAGCAAATGGCCCTGAGGTGCTGGAGTCTCAAA
TTCAAGCAATCTGATCACCAGTTCCTTCATCAGAGCAACGTCTTTCATCA
CATTAACAATATTTTGTCAAAGTCAGATGATGGCGATAGTGAAGAGAGTT
TTAGCATCAGTATACAGTCTGGCTTTGAAGCTATGAGTCAGGAATTATGC
ATAGTAATGTGCTTAAAGGACTTAACCAGCATTGTTGACATAAAAACTTC
AAGCCGACCTGCCATGATTGGCAGTTTGACAGACGGCTCCACAGAAACCT
TTTGGGAATCAGGAGATGAAGATAAAAACAAAACTAAGAACATCACCATC
AACTGTGTAAAAGGAATCAATGCCCGCTATGTGTCTGTTCACGTGGACAA
TTCCCGAGATCTTGGGAATAAAGTTACCTCAATGACCTTCTTAACTGGCA
AAGCAGTAGAAGATTTGTGCAGAATAAAGCAGGTTGATCTGGATTCCAGG
CACATTGGCTGGGTAACAAGTGAACTTCCAGGAGGGGATAATCACATCAT
AAAAATTGAATTAAAAGGCCCAGAAAATACACTGAGAGTTCGACAAGTCA
AAGTCCTGGGCTGGAAAGATGGTGAAAGCACAAAAATAGCTGGCCAGATT
TCAGCCAGTGTGGCCCAGCAGAGGAACTGTGAAGCTGAGACTCTGCGAGT
ATTCAGACTGATTACGTCTCAAGTATTTGGAAAGCTCATCTCTGGAGATG
CTGAACCTACACCAGAACAAGAGGAAAAAGCACTATTGTCATCACCTGAA
GGAGAAGAAAAAGTATACAATGCAACATCAGATGCTGACCTGAAAGAACA
TATGGTTGGAATCATATTCAGCAGGAGTAAGCTGACTAACTTACAAAAAC
AGGTGTGTGCTCATATTGTCCAAGCTATTCGCATGGAAGCTACCAGAGTC
CGTGAAGAATGGGAACATGCTATATCAAGCAAAGAAAATGCCAATTCTCA
GCCAAATGATGAAGATGCCTCCTCTGATGCCTACTGCTTTGAGCTGCTCT
CTATGGTTTTAGCACTGAGTGGCTCTAACGTTGGCCGGCAATATCTGGCT
CAACAGCTAACCCTGCTTCAGGATCTCTTCTCGCTGCTTCACACAGCCTC
TCCTAGAGTCCAGAGACAGGTAACCTCTTTACTAAGAAGAGTTTTGCCTG
AAGTAACCCCTAGTCGTCTGGCCAGCATCATAGGAGTGAAATCCCTCCCC
CCAGCAGATATCAGTGATATCATTCACTCAACAGAGAAAGGAGACTGGAA
TAAGCTGGGTATCTTGGACATGTTTCTAGGATGCATTGCCAAAGCACTCA
CTGTACAGCTAAAAGCCAAAGGAACCACCATCACTGGAACAGCTGGTACC
ACTGTGGGCAAAGGAGTTACAACAGTTACTCTTCCGATGATTTTCAATTC
CAGTTATCTCCGACGAGGTGAAAGTCATTGGTGGATGAAGGGCTCAACCC
CTACCCAGATCTCAGAGATCATCATTAAACTTATCAAGGATATGGCAGCA
GGTCATCTGTCAGAAGCTTGGTCCCGAGTGACAAAAAATGCTATTGCAGA
AACCATCATTGCCTTGACCAAGATGGAAGAAGAATTTAGGTCTCCAGTGA
GATGTATTGCAACAACTAGACTCTGGCTTGCTCTCGCATCCCTATGTGTT
CTTGATCAGGACCACGTAGATCGTCTCTCCTCGGGGAGATGGATGGGAAA
GGATGGACAACAAAAACAAATGCCTATGTGTGATAACCATGATGATGGTG
AAACTGCAGCAATCATTTTATGCAATGTCTGTGGAAATTTATGTACAGAC
TGTGACAGATTCCTTCACCTTCATCGAAGAACCAAAACTCATCAAAGACA
GGTCTTCAAAGAAGAAGAAGAAGCTATAAAGGTTGACCTTCATGAAGGTT
GTGGTAGAACCAAATTGTTCTGGTTGATGGCACTGGCAGATTCTAAAACA
ATGAAGGCAATGGTGGAATTCCGAGAACACACAGGCAAACCCACCACGAG
TAGCTCAGAAGCATGTCGCTTCTGTGGTTCCAGGAGTGGAACAGAGTTAT
CTGCTGTTGGCAGTGTTTGTTCTGATGCAGATTGCCAGGAATACGCTAAG
ATAGCCTGTAGTAAGACGCATCCTTGTGGCCATCCATGCGGGGGTGTTAA
AAACGAAGAGCACTGTCTGCCCTGTCTACACGGCTGTGACAAAAGTGCCA
CAAGCCTGAAGCAAGACGCCGATGACATGTGCATGATATGTTTCACCGAA
GCGCTCTCGGCAGCACCAGCCATTCAGCTGGATTGTAGTCACATATTCCA
CTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGGCTTGGCCCAAGGA
TAACATTTGGATTTATATCTTGTCCCATTTGCAAGAACAAAATTAATCAC
ATAGTACTAAAAGACCTACTTGATCCAATAAAAGAACTCTATGAGGATGT
CAGAAGAAAAGCCTTAATGAGATTGGAATATGAAGGTCTGCATAAGAGTG
AAGCTATCACAACTCCTGGTGTGAGGTTTTATAATGACCCAGCTGGCTAT
GCAATGAATAGATATGCATATTATGTGTGCTACAAATGCAGAAAGGCATA
TTTTGGTGGTGAAGCTCGCTGCGATGCTGAGGCTGGACGGGGAGATGATT
ATGATCCCAGAGAGCTCATTTGTGGTGCCTGTTCTGATGTTTCCAGGGCT
CAGATGTGTCCCAAACATGGCACAGACTTTTTGGAATATAAATGTCGCTA
CTGCTGTTCAGTGGCTGTTTTTTTCTGTTTTGGAACAACACATTTTTGTA
ATGCTTGTCATGATGATTTTCAAAGAATGACTAGCATTCCTAAGGAAGAA
CTACCACACTGTCCTGCAGGTCCCAAAGGCAAGCAGTTAGAAGGAACTGA
ATGTCCACTCCATGTTGTTCATCCACCCACTGGGGAAGAGTTTGCTCTGG
GATGTGGAGTGTGCAGAAATGCCCACACTTTTTAGgcggccgc

Amino Acid Sequence

MEQKLISEEDLGSFEEFASMPVPDGSVAAAGLGLGLPAADSRGHYQLLLSGRALADRYRRIYTAALNDR
DQGGGSAGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENLENTVIIPD
IKLHSNPSAFNIYCNVRHCVLEWQKKEISLAAASKNSVQSGESDSDEEEE
SKEPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQP
EGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVAS
WGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQIQ
DWFSNGIKKAALMHKWPLKEISVDEDDQCLLQNDGFFLYLLCKDGLYKIG
SGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDVNNHSMTAIRI
SPETLEQDGTVMLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATS
TEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGR
EFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVG
HDGSHALLVAEDGSIFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGK
IVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVTDLKGHFVTQVAM
GKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDE
DLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRP
DRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPL
DLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEEGRGPLVFAGPIFMN
HREQALARLRSHPAQLKHKRDKHKDGSGERGEKDASKITTYPPGSVRFDC
ELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGCPTLVQA
LPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDVPYWNAKPA
PMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFASKHIIG
LVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQGFGVCLDPVYDVIW
RFRPNTRELWCYNAVVADARLPSAADMQSRCSILSPELALPTGSRALTTR
SHAALHILGCLDTLAAMQDLKMGVASTEEETQAVMKVYSKEDYSVVNRFE
SHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRGEYTAKIKLFELGPD
GGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQAGWWYVAWARVS
GPSSDCGSHGQASITTDDGVVFQFKSSKKSNNGTDVNAGQIPQLLYRLPT
SDGSASKGKQQTSEPVHILKRSFARTVSVECFESLLSILHWSWTTLVLGV
EELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYTCEIYPVSATGKAVV
EETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDPQGYLSQPLSLLEAV
LQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQDIQEANFKTSSSRLL
AAVMSALCHTSVKLTSIFPIAYDGEVLLRSIVKQVSTENDSTLVHRFPLL
VAHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVRNSLRRENELFSSHL
VSNTCGLLASIVSELTVSALGSEVDGLNSLHSVKASANRFTKTSQGRSWN
TGNGSPDAICFSVDKPGIVVVGFSVYGGGGIHEYELEVLVDDSEHAGDST
HSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLKENVKYAVRLRNYGS
RTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTRGQIPQILYYRSEFD
GDLQSQLLSKANEEDKNCSRALSVVSTVVRASKDLLHRALAVDADDIPEL
LSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLVQQLLPSVAILNQKY
APPAFNPNQSTDSTTGNQPEQGLSACTTSSHYAVIESEHPYKPACVMHYK
VTFPECVRWMTIEFDPQCGTAQSEDVLRLLIPVRTVQNSGYGPKLTSVHE
NLNSWIELKKFSGSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGF
KCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALMKKDLALPIGNELEE
DLEILEEAALQVCKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQS
FLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTIT
VQTKDQYGDVVHVPNMKVEVKAVPVSQKKMSLQQDQAKKPQRIPGSPAVT
AASSNTDMTYGGLASPKLDVSYEPMIVKEARYIAITMMKLEVYENYSFEE
LRFASPTPKRPSENMLIRVNNDGTYCANWTPGAIGLYTLHVTIDGIEIDA
GLEVKVKDPPKGMIPPGTQLVKPKSEPQPNKVRKFVAKDSAGLRIRSHPS
LQSEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVPNMNGYTEAW
CLSFNQHLGKSLLVPVDESKTNTDDFFKDINSCCPQEATMQEQDMPFLRG
GPGMYKVVKTGPSGHNIRSCPNLRGIPIGMLVLGNKVKAVGEVTNSEGTW
VQLDQNSMVEFCESDEGEAWSLARDRGGNQYLRHEDEQALLDQNSQTPPP
SPFSVQAFNKGASCSAQGFDYGLGNSKGDRGNISTSSKPASTSGKSELSS
KHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSR
SLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSG
ASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKM
PLTEPLRGRSTSPKPKSVPKDSTDSPGSENRAPSPHVVQENLHSEVVEVC
TSSTLKTNSLTDSTCDDSSEFKSVDEGSNKVHFSIGKAPLKDEQEMRASP
KISRKCANRHTRPKKEKSSFLFKGDGSKPLEPAKQAMSPSVAECARAVFA
SFLWHEGIVHDAMACSSFLKFHPELSKEHAPIRSSLNSQQPTEEKETKLK
NRHSLEISSALNMFNIAPHGPDISKMGSINKNKVLSMLKEPPLHEKCEDG
KTETTFEMSMHNTMKSKSPLPLTLQHLVAFWEDISLATIKAASQNMIFPS
PGSCAVLKKKECEKENKKSKKEKKKKEKAEVRPRGNLFGEMAQLAVGGPE
KDTICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNC
GDGGIGGSTWYLVCDRCREKYLREKQAAAREKVKQSRRKPMQVKTPRALP
TMEAHQVIKANALFLLSLSSAAEPSILCYHPAKPFQSQLPSVKEGISEDL
PVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVTP
DASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYDK
QHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSKGE
RNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVIQ
TTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEKDTRVCEHPL
SDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKFK
QSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFEAMSQELCIV
MCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITINC
VKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIKQVDLDSRHI
GWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQISA
SVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEGE
EKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVRE
EWEHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSNVGRQYLAQQ
LTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASIIGVKSLPPA
DISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTTV
GKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIKLIKDMAAGH
LSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVLD
QDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNVCGNLCTDCD
RFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMK
AMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKIA
CSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDMCMICFTEAL
SAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHIV
LKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDPAGYAM
NRYAYYVCYKCRKAYFGGEARCDAEAGRGDDYDPRELICGACSDVSRAQM
CPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEELP
HCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29760
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CORRECTED aa Met1-Lys2339 amplified from 29671 with NW5195/96 and inserted downstream of the N-terminal MYC tag in Nhe/Xho cut 58354 backbone (containing Val2340-Phe4640) using NEBbuilder to give MYC tagged MYCBP2 full-length with the following changes relative to Virdee group ref sequence (Virdee MYCBP2 ref attached below):; Contains listed SNP A1667V - this SNP equates to A1705V in NM_015057.4. Silent mutations: K125 (AAA > AAG), Y1096 (TAT > TAC), L1739 (TTG > TTA), C2006 (TGT > TGC). There is an artificial Xho1 site (to be removed next) at nt 6966 (deriving from strategy to join the two half lengths of full-length MYCBP2 Nhe1-Xho1-Not1). ; N-terminal MYC tag and extraneous residues upstream of the native start site (position M20 of fusion) derives from Parental 44556 - ; ***possible 1bp deletion in N-terminal tag which would have frame implications - under investigation while next step to remove Xho1 site is underway***
• Low Temp: No