cDNA Clone IRAK3

• Plasmid: pCMV5 FLAG IRAK3 F177G C291H S293D
• Parent Plasmid: pCMV5 FLAG
• Expressed: FLAG IRAK3 F177G C291H S293D
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACAC
GCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTC
TGGACAGCTGCGACGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTT
TCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGG
TAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGA
CCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCT
ATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAG
TTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATG
TCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTG
CTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTT
CCACAAAGACTTCCTAATTGGAGAAGGAGAGATTggTGAGGTATACAGAG
TGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAA
AAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGT
TTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTA
CAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACA
CTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCA
CATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGC
ACAACGTTCAACCATGCTCGGTCATCcaTGGCgaTATATCAAGTGCAAAC
ATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGC
ACACTTCCGGTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCA
GCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAG
GGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAAT
GGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCC
AGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCA
TGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTC
TGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGT
TAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCC
AGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTG
TCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATG
ATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATA
GACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTAT
GTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTT
GCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATA
GTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTC
AGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTT
CCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAAgcg
gccgc

Amino Acid Sequence

MDYKDDDDKGSMAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGAL
GWRGLAERLSSSWLDVRHIEKYVDQGKSGTRELLWSWAQKNKTIGDLLQV
LQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLI
PEHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIGEVYRVEIQNLTY
AVKLFKQEKKMQCKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCL
IYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHYLHNVQPCSV
IHGDISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLW
YMPEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRE
LMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEV
LNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNL
LPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSC
EESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDE
YEQYKKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: One silent mutation at G40 (c120t) and a SNP at I147V.
• Low Temp: No