cDNA Clone DENND4C [mouse]

• Plasmid: pET15b 6HIS SUMO DENND4C Mouse 32-671
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO DENND4C mouse 32-671
• Genbank: A6H8H2
• Tag: 6His
• Tag: SUMO
• Species: Mouse

Sequence of Insert

GGATCTATGACAGATACTAACTCGATTGGTCCTAAAGCTCCAATCACAGACATTGCTGTTATTATCAAATCAGCTGGGGAAACTGTACCTGAAGGCTACACCTGTGTGGAAGCCACTCCATCAGCACTCCAAGCAAACTTGAATTATGGAAGTCTGAAGAGCCCAGAGCTTTTCCTGTGCTACAGGAGAGGGAGAGATAAGCCGCCCCTTACAGATATTGGCGTGCTATATGAAGGGAAAGAAAGGCTTATGCCAGGATGTGAAGTGATCCAAGCCACGCCGTACGGCCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCTCAAAGAATCTTTATCACCTATCGAAGGGCTCCCCCAGTGCGGTCCCAGAATTCCTTGGCCGTCACTGATATCTGTGTTATTATAACCAGTAAAGGAGAAACCCCTCCGCATACTTTCTGCAAAGTGGATAAAAATTTAAACTGTGGCATGTGGGGTTCCAACGTGTTTCTGTGTTACAAGAAGTCTGTGCCTGCTTCAAATGCAATAGCATATAAGGCTGGTTTGATTTTTCGATATCCGGAAGAAGACTATGAGTCTTTTCCGCTCTCACCATCTGTGCCTCTCTTCTGCCTTCCTATGGGAGCCACTATTGAATGCTGGGATCCTCAAATCAAATACCCACTTCCTGTTTTCTCAACATTTGTCCTGACAGGGTCTTCAGCTGAAAAGGTATATGGAGCTGCCATCCAGTTTTATGAACCTTACTCTCAAGAACGTCTAACAGAGAAACAACTTACACAGCTGGGCCTATTGACCCTCGTGGAGAAAAGAGTGGTCTCCAAACCCATCAATTCAAACAAGTGCATTTGTTTGCTTTCACACTGGCCTTTTTTTGAAGCTTTTAAGAATTTTCTTATGTTTATCTACAAAGTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTATGCAAAATATCCCTTTTCCTTCACCACAAAGACCAAGAATCCTCATCCAGCTATCAGTCCATGATGCATTTATATTATCACAGCCAGTGTCCACACCTTTACCATTAAGTGGCGCCAACTTTAGCTCCTTGCTGATGAATCTGGGTCCTGAGAACTGCGCGACATTGCTGCTGCTGGTTTTGCTCGAGAGTAAGATTCTGCTGCACTCTCTGAGGCCAGCGGTACTAACTGGGGTAGCCGAAGCTGTCGTTGCTATGATCTTTCCATTTCAGTGGCAGTGCCCATATATCCCCCTCTGTCCTCTTTCATTGGCTGGAGTGCTTAGTGCGCCTTTACCATTTATAGTTGGAGTGGACTCAAGGTACTTTGATCTTCATGACCCACCACAAGATGTTGTCTGCATTGACTTGGATACGAACACATTATATGTAGCTGATGAAAGAAAGAACATAAACTGGAAGCAGCTTCCCAAAAGGCCGTGCAAGAGTCTGCTTGGCACCCTGAGGAGGCTGTATCAGCAGCTCTGCTCAGTTCACCGAAAACCTCAAGAAAGTTCAGCAATTGAGATGACTCCAATTGAAGCAGATTACTCTTGGCAAAAAAAGATGACACAATTGGAGATGGAAATTCAGGAGACATTTTTGCGATTTATGGCGTCTATTTTAAAAGGGTACAGGTCATATCTCAGACCAATCACAGAAGCTCCTTCTAATAAAGCCACAGCTGCGGATTCATTGTTTGATCGACAGGGTTTTTTGAAAAGTCGGGATCGTGCCTATACAAAATTCTATACTCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAGTTTTGTAAGTGATAAAGATACTGGATTGGCATTTTTTGATGATTGCATCGAGAAGTTGTTTCCAGATAAAGGTGTAGAGAGAACAGAAAAGGTTGACTTGGATTCAGCAGAAGACACCTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMTDTNSIGPKAPITDIAVIIKSAGETVPEGYTCVEATPSALQANLNYGSLKSPELFLCYRRGRDKPPLTDIGVLYEGKERLMPGCEVIQATPYGRCANVNNSSTTSQRIFITYRRAPPVRSQNSLAVTDICVIITSKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNAIAYKAGLIFRYPEEDYESFPLSPSVPLFCLPMGATIECWDPQIKYPLPVFSTFVLTGSSAEKVYGAAIQFYEPYSQERLTEKQLTQLGLLTLVEKRVVSKPINSNKCICLLSHWPFFEAFKNFLMFIYKVSVSGPHPLPIEKHISHFMQNIPFPSPQRPRILIQLSVHDAFILSQPVSTPLPLSGANFSSLLMNLGPENCATLLLLVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNTLYVADERKNINWKQLPKRPCKSLLGTLRRLYQQLCSVHRKPQESSAIEMTPIEADYSWQKKMTQLEMEIQETFLRFMASILKGYRSYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYTKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDCIEKLFPDKGVERTEKVDLDSAEDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62662
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker after senp cleavage deleted to make clone flush
• Low Temp: No