cDNA Clone Mindy3

• Plasmid: pGEX Mindy3 C139A (Drosophila)
• Expressed: GST-C3-Mindy3 C139A (Drosophila)
• Genbank: NM_133110.3
• Tag: GST
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCATGAACGAGAAAATCGTGCGGGAGCAGCGCGGCGGCGAAGATTCCCCATCCTCCGTCTCGGCGAAAAGTGCAACGGCAGCGGCGTCAGCGTCGACAGCGTCGATGACGTTTGCCTCGGCGGCCTTGGAATCGCACAAGACGACCACCATCACGACCGCCAGCAGCAGCCCTCGCACCAGTGGAGCAAGTGCATCCGCCTCGTCATCCTCTCGATCCGCATCTGCACCTGCATCGTCGTCCAGCCAGCAGGAAAAGCAACCGATGCTGAATGCCACCGATATGCGCGAACTGCGCGAAATCAAGCAGTTGCTGTGGGGCGACAATGTGCGCGAAGATGTATTCAAGCGTTGGTCTCAAGGATTCGAGTTTAGTAAAGTGGAACCGTCTGCATTGGTGCAGAAGCAGGGCGGTCCTGCTGCTGTGATAGCGCCGGTGCAGGCGTACTTGCTTAAGATTATTATCATGGACTTGCCAGGCATCAAGCTCTCGGAGATTTCCCTCGACAAATCGCAGAACCTGCTGATCCAGGCATTGTGTGACATCCTGAAGAATTGTCGGGCGCCGCGGTACCGCATCGTCCATCTCTTGCGCCGACGAGGAAACGCTACAGAGGCTGGATCAACCAAAAAGCGCTCACCGGCTGGCGAGGAAGAATCTGCTTTAGCTGGCCAAGCGGCAGGATCTTCCGAAGAGGTGGAGGAGGCGGCAGAAGCCACTCCCGCCTCGGTCAGCAAGTTGTCCCAGGCCCTGCAGCTCGAACACGATATGCACCAGGAACTGTCGCCGGATGAGTTCCACGAGCGCCTGCACACGCTCCACTTTAAGAATATCGCAGCCGTGGCCCGCTACTATATGGAGAATTACGACCAGCTGGCGCACACATACGGCGTGCTGCTGTTCATGTACTCGGTGTTCCTCACCAAGGGCTTGGAGCTGGTTGCGGCCGATATATCGGACACGTCGGAGCCCCTGATACACAGCACATACGGCTATGGCGGCCAGTCGCTTATTAACCTGATGCTAACTGGGCGAGCAGTTGCCCATGTCTGGGATAATGAGCAGGACGTTGGCGGACTGAAGCTGCGTGGCATCTGTGAGCAGAGCGACATCGGCTTTATAACACTGATGGAGGAAATGCGTTACTGCACGGTGGGCTCCTTCTTCAAGAACCCACGCTATCCCGTATGGGTGATGGGGTCCGATACGCATTTAACCGTTCTGTTCAGCAACGAAAAGAGGCTTGTCTCACCAGAGACGCCCTCGGAAACTGGTCGTCGGATCTTCAAATCGTACGACCCGGAGGGCAACAATTTCATATCGACCACAATGCTGCGCGAAGTTCTCATCGCCTTGAATCTGGTCAGCGAGCCGGCCTATGTCGCCCTCATGCAGAAGCGTCTTGATCCGGAGAACTTGGGCATTATACTGCTAAATGCGTTCATGGACGAGTTCTTCCCGCTGGAGAGCCGCTCCACGCCGGATACGTTCGAGCTGATGCACTACAACGGCATACCGGGCTCCAATGAGAACAACAAGGTGCGCTACTATTGCGGCACGGCCATTTTGCTGGAAGGCGATCTGAAGTCGGTCTGCACCTCGAACCCGATGGTCACCTGCCTGCAGACCAAGTGGCCAAATATCGAAATAAACTGGCACGACGGCCATATGCCCTCCTTGAATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNEKIVREQRGGEDSPSSVSAKSATAAASASTASMTFASAALESHKTTTITTASSSPRTSGASASASSSSRSASAPASSSSQQEKQPMLNATDMRELREIKQLLWGDNVREDVFKRWSQGFEFSKVEPSALVQKQGGPAAVIAPVQAYLLKIIIMDLPGIKLSEISLDKSQNLLIQALCDILKNCRAPRYRIVHLLRRRGNATEAGSTKKRSPAGEEESALAGQAAGSSEEVEEAAEATPASVSKLSQALQLEHDMHQELSPDEFHERLHTLHFKNIAAVARYYMENYDQLAHTYGVLLFMYSVFLTKGLELVAADISDTSEPLIHSTYGYGGQSLINLMLTGRAVAHVWDNEQDVGGLKLRGICEQSDIGFITLMEEMRYCTVGSFFKNPRYPVWVMGSDTHLTVLFSNEKRLVSPETPSETGRRIFKSYDPEGNNFISTTMLREVLIALNLVSEPAYVALMQKRLDPENLGIILLNAFMDEFFPLESRSTPDTFELMHYNGIPGSNENNKVRYYCGTAILLEGDLKSVCTSNPMVTCLQTKWPNIEINWHDGHMPSLN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59950
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate C139 to alanine in 59102. Cheers!
• Low Temp: No