cDNA Clone MCC

• Synonyms: Colorectal mutant cancer protein , CRCM, MCC regulator of WNT signaling pathway
• Plasmid: pcDNA5D-FRT/TO HA-MCC
• Parent Plasmid: pcDNA5D-FRT/TO HA
• Expressed: HA-MCC
• Genbank: NM_002387.3 → NP_002378.2 AC079465.5 (Uniprot P23508.2)
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGAATTCCGGAGTTGCCATGAAATATGGAAACGACTCCTCGGC
CGAGCTGAGTGAGCTCCATTCAGCAGCCCTGGCATCACTAAAGGGAGATA
TAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGACCTT
CTGGAAAAGAAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAG
AGAGCATGAGGATGTCCAGGAGCGAACGACGCTTCGCTATGAGGAACGCA
TCACAGAGCTCCACAGCGTCATTGCGGAGCTCAACAAGAAGATAGACCGT
CTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCAGAACTGCGATC
AGAACTCAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATGG
ACCAAGACCAGACCTCTGTCTCTATCCCCGAAAACCAGTCTACCATGGTT
ACTGCTGACATGGACAACTGCAGTGACCTGAACTCAGAACTGCAGAGGGT
GCTGACAGGGCTGGAGAATGTTGTCTGCGGCAGGAAGAAGAGCAGCTGCA
GCCTCTCCGTGGCCGAGGTGGACAgGCACATTGAGCAGCTCACCACAGCC
AGCGAGCACTGTGACCTGGCTATTAAGACAGTCGAGGAGATTGAGGGGGT
GCTTGGCCGGGACCTGTATCCCAACCTGGCTGAAGAGAGGTCTCGGTGGG
AGAAGGAGCTGGCTGGGCTGAGGGAAGAGAATGAGAGCCTGACTGCCATG
CTGTGCAGCAAAGAGGAAGAACTGAACCGGACTAAGGCCACCATGAATGC
CATCCGGGAAGAGCGGGACCGGCTCCGGAGGAGGGTCAGAGAGCTTCAAA
CTCGACTACAGAGCGTGCAGGCCACAGGTCCCTCCAGCCCTGGCCGCCTC
ACTTCCACCAACCGCCCGATTAACCCCAGCACTGGGGAGCTGAGCACAAG
CAGCAGCAGCAATGACATTCCCATCGCCAAGATTGCTGAGAGGGTGAAGC
TATCAAAGACAAGGTCCGAATCGTCATCATCTGATCGGCCAGTCCTGGGC
TCAGAAATCAGTAGCATAGGGGTATCCAGCAGTGTGGCTGAACACCTGGC
CCACTCACTTCAGGACTGCTCCAATATCCAAGAGATTTTCCAAACACTCT
ACTCACACGGATCTGCCATCTCAGAAAGCAAGATTAGAGAGTTTGAGGTG
GAAACAGAACGGCTGAATAGCCGGATTGAGCACCTCAAATCCCAAAATGA
CCTCCTGACCATAACCTTGGAGGAATGTAAAAGCAATGCCGAGAGGATGA
GCATGCTGGTGGGAAAATACGAATCCAATGCCACAGCGCTGAGGCTGGCC
TTGCAGTACAGCGAGCAGTGCATCGAAGCCTACGAACTCCTCCTGGCGCT
GGCAGAGAGTGAGCAGAGCCTCATCCTGGGGCAGTTCCGAGCGGCGGGCG
TGGGGTCCTCCCCTGGAGACCAGTCGGGGGATGAAAACATCACTCAGATG
CTCAAGCGAGCTCATGACTGCCGGAAGACAGCTGAGAATGCTGCCAAGGC
CCTGCTCATGAAGCTGGACGGCAGCTGTGGGGGAGCCTTTGCCGTGGCCG
GCTGCAGCGTGCAGCCCTGGGAGAGCCTTTCCTCCAACAGCCACACCAGC
ACAACCAGCTCCACAGCCAGTAGTTGCGACACCGAGTTCACTAAAGAAGA
CGAGCAGAGGCTGAAGGATTATATCCAGCAGCTCAAGAATGACAGGGCTG
CGGTCAAGCTGACCATGCTGGAGCTGGAAAGCATCCACATCGATCCTCTC
AGCTATGACGTCAAGCCTCGGGGAGACAGCCAGAGGCTGGATCTGGAAAA
CGCAGTGCTTATGCAGGAGCTCATGGCCATGAAGGAGGAGATGGCCGAGT
TGAAGGCCCAGCTCTACCTACTGGAGAAAGAGAAGAAGGCCCTGGAGCTG
AAGCTGAGCACGCGGGAGGCCCAGGAGCAGGCCTACCTGGTGCACATTGA
GCACCTGAAGTCCGAGGTGGAGGAGCAGAAGGAGCAGCGGATGCGATCCC
TCAGCTCCACCAGCAGCGGCAGCAAAGATAAACCTGGCAAGGAGTGTGCT
GATGCTGCCTCCCCAGCTCTGTCCCTAGCTGAACTCAGGACAACGTGCAG
CGAGAATGAGCTGGCTGCGGAGTTCACCAACGCCATTCGTCGAGAAAAGA
AGTTGAAGGCCAGAGTTCAAGAGCTGGTGAGTGCCTTGGAGAGACTCACC
AAGAGCAGTGAAATCCGACATCAGCAATCTGCAGAGTTCGTGAATGATCT
AAAGCGGGCCAACAGCAACCTGGTGGCTGCCTATGAGAAAGCAAAGAAAA
AGCATCAAAACAAACTGAAGAAGTTAGAGTCGCAGATGATGGCCATGGTG
GAGAGACATGAGACCCAAGTGAGGATGCTCAAGCAAAGAATAGCTCTGCT
AGAGGAGGAGAACTCCAGGCCACACACCAATGAAACTTCGCTTTAAgcgg
ccgc

Amino Acid Sequence

MYPYDVPDYAGSMNSGVAMKYGNDSSAELSELHSAALASLKGDIVELNKR
LQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELHSV
IAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRSMDQDQTSV
SIPENQSTMVTADMDNCSDLNSELQRVLTGLENVVCGRKKSSCSLSVAEV
DRHIEQLTTASEHCDLAIKTVEEIEGVLGRDLYPNLAEERSRWEKELAGL
REENESLTAMLCSKEEELNRTKATMNAIREERDRLRRRVRELQTRLQSVQ
ATGPSSPGRLTSTNRPINPSTGELSTSSSSNDIPIAKIAERVKLSKTRSE
SSSSDRPVLGSEISSIGVSSSVAEHLAHSLQDCSNIQEIFQTLYSHGSAI
SESKIREFEVETERLNSRIEHLKSQNDLLTITLEECKSNAERMSMLVGKY
ESNATALRLALQYSEQCIEAYELLLALAESEQSLILGQFRAAGVGSSPGD
QSGDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPW
ESLSSNSHTSTTSSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTML
ELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMAELKAQLYL
LEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSG
SKDKPGKECADAASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQ
ELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNKLK
KLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65457
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 2; Clone contains the following changes compared to the NCBI ref sequence:; Silent changes at Thr75 (ACA > ACG), Asn511 (AAC > AAT), Asp691 (GAC > GAT) and Ala708 (GCC > GCT); Also contains K190R listed SNP
• Low Temp: No