cDNA Clone ALPK1

• Plasmid: pcDNA5-FRT/TO-FLAG-ALPK1 [K1067M]
• Parent Plasmid: pcDNA5-FRT/TO-FLAG
• Expressed: FLAG-ALPK1 [K1067M]
• Genbank: NM_025144.4 → NP_079420.3 GI: 1519314014
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTGGAAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCCAGCGAGTTAAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCCTTCGTGCCTGAAAAGTGGCAGTACAAACAAGCCGTGGGCCCAGAGGACAAAACAAACCTGAAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGTCCCTGAGGGCCTCCATCCTCGCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTTCCTGTATGGGCTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCCACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTTTTAAAAGCAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACCTGGCTGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGAGGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGAGTTAATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGGGCCTCTCCACGTCGCTAGGTATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTTTAAAAACAATCCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAAGCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTGAATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTGAAAAACTTACATCTGTGTGAAGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTTACTGGAAAACAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGTCTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAGCTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGTCAGGACAGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAAGTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGGTTGGATAAACTTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCACAGCACCATACTTCGGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGAAAGATGCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAAAACATAGATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGACACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAGAAACTGGACCCATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCGGACTACAGCAATGGTGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCCAGTGCTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTATCACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAGTGTTCCACTGCCTTGTCTGAGGAGCTAGAGAATGACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAGCTTCATGATCTCTCTCTTCAGGAACCCAACAATGACAATTTGGAGCCTTCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCAGGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAGGAAGTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCTTCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATATGGGCACACATCCTTCAGTCCAAAAAGAAGAAGCCTTTGAAATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGGCAGGGGAAAGAGCAGGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTCCCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCACCCTTAGACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCATGCTGCCATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCAGAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGCATGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCGCCTGGTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTCCTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGCAGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAATCACCTGCATTTTCCAGTGGTTCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCATTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTTGAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAATACGGGGGTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAAAAATCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTGAAGAAAAAAGGCAGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGGTATGTTGGGAtgGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATGTGGAGCGACAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAGGACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAATTGTCAAATAACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTGGCCTATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGTTGGGTAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCACTCCGTTGATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAATAACCAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAGAAACCATGCACATAGgcggccgc

Amino Acid Sequence

MDYKDDDDKGSMNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRC
RALLPSELRTLIQEAKEMKWPFVPEKWQYKQAVGPEDKTNLKDVIGAGLQ
QLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQP
ATPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDK
VLVQSVCIQIRGQILQKLGMWYEAAELIWASIVGYLALPQPDKKGLSTSL
GILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLLSAAEACKLAAAFS
AYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTK
RDDEPVTGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKL
YNFSTSSRSQDREALSQEVMSVIAQVKEHLQVQSFSNVDDRSYVPESFEC
RLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKDAKTGVC
ITALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNW
THSDAFRVSLDQDVETETEPSDYSNGEGAVFNKSLSGSQTSSAWSNLSGF
SSSASWEEVNYHVDDRSARKEPGKEHLVDTQCSTALSEELENDREGRAMH
SLHSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAG
LLEGAPEGIQEVRNMGPRNTSAHSRPSYRSASWSSDSGRPKNMGTHPSVQ
KEEAFEIIVEFPETNCDVKDRQGKEQGEEISERGAGPTFKASPSWVDPEG
ETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQNPDSRKSGGPV
AEQGIDPDASTVDEEGQLLDSMDVPCTNGHGSHRLCILRQPPGQRAETPN
SSVSGNILFPVLSEDCTTTEEGNQPGNMLNCSQNSSSSSVWWLKSPAFSS
GSSEGDSPWSYLNSSGSSWVSLPGKMRKEILEARTLQPDDFEKLLAGVRH
DWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLGDYLT
VKKKGRQRNAFWVHHLHQEEILGRYVGMDYKEQKGLWHHFTDVERQMTAQ
HYVTEFNKRLYEQNIPTQIFYIPSTILLILEDKTIKGCISVEPYILGEFV
KLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGL
IYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSM
EKPCT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-FLAG-ALPK1 [K1067M] 456.67 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65680
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41457; [K1067M] Kinase Dead
• Low Temp: No