cDNA Clone ALPK1

• Plasmid: pcDNA5-FRT/TO-HA-ALPK1
• Parent Plasmid: pcDNA5-FRT/TO-HA
• Expressed: HA-ALPK1
• Genbank: NM_025144.4 → NP_079420.3 GI: 1519314014
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAA
GCAAGTGCTGGATCAGCTCTTGTTGGAAGCGCCAGATGTGTCGGAAGAGG
ACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCCAGCGAGTTAAGG
ACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCCTTCGTGCCTGAAAA
GTGGCAGTACAAACAAGCCGTGGGCCCAGAGGACAAAACAAACCTGAAGG
ATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGTCCCTGAGGGCCTCC
ATCCTCGCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGA
CCGGTTCCTGTATGGGCTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCA
AAGGTCTCCACAAGTTGCAGCCAGCCACGCCAATTGCCCCGCAGGTGGTT
ATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTTTTAAAAGCAGA
GTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACCTGGC
TGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAGTCGGTCTGTATACAG
ATCAGAGGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGA
GTTAATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCGGATA
AAAAGGGCCTCTCCACGTCGCTAGGTATACTGGCAGACATCTTTGTTTCC
ATGAGCAAGAACGATTATGAAAAGTTTAAAAACAATCCACAAATTAATTT
GAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAAGCCT
GCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACA
GCTGTGAATATCCGTGGCACGTGTTTATTGTCCTACAGTAGTTCAAATGA
CTGTCCTCCAGAATTGAAAAACTTACATCTGTGTGAAGCCAAAGAGGCCT
TTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTTACTGGAAAA
CAGGAGCTTCACAGCTTTGTCAAAGCTGCTTTCGGTCTCACCACAGTGCA
CAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCAGCAAGTCAGCTCT
GTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGT
CAGGACAGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGT
GAAGGAACATTTACAAGTTCAAAGCTTCTCAAATGTAGATGACAGATCTT
ATGTTCCCGAGAGTTTCGAGTGCAGGTTGGATAAACTTATCTTGCATGGG
CAAGGGGATTTCCAAAAAATCCTTGACACCTATTCACAGCACCATACTTC
GGTGTGTGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGA
AAGATGCAAAAACAGGAGTCTGCATCACTGCTCTAAAAACAGAAATAAAA
AACATAGATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGA
CACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGGGAACTCA
GAAGGGGAGGAAGGAGAAACTGGACCCATTCTGATGCATTTCGAGTCTCC
TTGGATCAAGATGTGGAGACTGAGACTGAGCCATCGGACTACAGCAATGG
TGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCCAGTG
CTTGGAGCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTG
AATTATCACGTTGACGACAGGTCAGCCAGAAAAGAGCCTGGCAAAGAACA
TCTGGTGGACACTCAGTGTTCCACTGCCTTGTCTGAGGAGCTAGAGAATG
ACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAGCTTCATGATCTC
TCTCTTCAGGAACCCAACAATGACAATTTGGAGCCTTCTCAAAATCAGCC
ACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCATAATACCCCAGGCA
TTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATC
CAGGAAGTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCCAGACC
CTCATATCGTTCTGCTTCTTGGTCTTCTGATTCTGGTAGGCCCAAGAATA
TGGGCACACATCCTTCAGTCCAAAAAGAAGAAGCCTTTGAAATAATTGTT
GAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGGCAGGGGAAAGAGCA
GGGAGAAGAAATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTC
CCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAAGCACTGAAGATGCA
CCCTTAGACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCAT
GCTGCCATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAAAATCCTGACT
CCAGAAAAAGTGGTGGCCCAGTCGCAGAGCAGGGCATCGACCCTGATGCC
TCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGCATGGATGTTCCCTG
CACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCGCCTG
GTCAGAGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTC
CCTGTCCTCAGCGAGGACTGCACTACCACAGAGGAAGGAAATCAGCCTGG
AAACATGCTAAACTGCAGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGC
TGAAATCACCTGCATTTTCCAGTGGTTCTTCTGAGGGGGACAGCCCTTGG
TCCTATCTGAATTCCAGTGGGAGTTCTTGGGTTTCATTGCCGGGAAAGAT
GAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTTGAAA
AGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAAT
ACGGGGGTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTT
GTTAAAATATTCAAAAAAATCTGAACTGTGGACGGCCCAGGAAACTATTG
TCTATTTGGGGGACTACTTGACTGTGAAGAAAAAAGGCAGACAAAGAAAT
GCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGGTATGT
TGGGAAAGACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATG
TGGAGCGACAGATGACCGCACAGCACTATGTGACAGAATTTAACAAGAGA
CTCTATGAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAAT
ACTACTGATTTTAGAGGACAAGACAATAAAGGGATGTATCAGTGTGGAGC
CTTACATACTGGGAGAATTTGTAAAATTGTCAAATAACACGAAAGTGGTG
AAAACAGAATACAAAGCCACAGAATATGGCTTGGCCTATGGCCATTTTTC
TTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGTTGGG
TAACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCAC
TCCGTTGATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAATTTT
TTACTTCTTTAATAACCAGCATGTGGAATGTAATGAAATCTGCCATCGTC
TTTCTTTGACTAGACCTTCAATGGAGAAACCATGCACATAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKWQYKQAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQELHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQVKEHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKDAKTGVCITALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQDVETETEPSDYSNGEGAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHLVDTQCSTALSEELENDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAGLLEGAPEGIQEVRNMGPRNTSAHSRPSYRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKDRQGKEQGEEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQNPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSMDVPCTNGHGSHRLCILRQPPGQRAETPNSSVSGNILFPVLSEDCTTTEEGNQPGNMLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMRKEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSMEKPCT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-HA-ALPK1228.41 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65773
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41456
• Low Temp: No