Plasmid
pcDNA5-FRT-TO-MBP-3FLAG-ALPK1 K1067M-C-FLAG
Parent Plasmid
pcDNA5-FRT-TO-MBP
Expressed
MBP-3FLAG-ALPK1 K1067M-FLAG
Genbank
80216
Tag
FLAG
MBP
Species
Human
Amino Acid Sequence
View Sequence
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGGMDYKDHDGDYKDHDIDYKDDDDKGSMNNQKVVAVLLQECKQ
VLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKW
QYKQAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDR
FLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARISVNSGKLLKAEY
ILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAEL
IWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLS
LLKEFDHHLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDC
PPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQELHSFVKAAFGLTTVHR
RLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQVK
EHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSV
CEVFESDCGNNKNEQKDAKTGVCITALKTEIKNIDTVSTTQEKPHCQRDT
GISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQDVETETEPSDYSNGE
GAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHL
VDTQCSTALSEELENDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQ
QQMPLTPFSPHNTPGIFLAPGAGLLEGAPEGIQEVRNMGPRNTSAHSRPS
YRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKDRQGKEQG
EEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISML
PCSSFTPNWPVQNPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSMDVPCT
NGHGSHRLCILRQPPGQRAETPNSSVSGNILFPVLSEDCTTTEEGNQPGN
MLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMR
KEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLL
KYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVG
MDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTIL
LILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSY
EFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFY
FFNNQHVECNEICHRLSLTRPSMEKPCTDYKDDDDK*
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGGMDYKDHDGDYKDHDIDYKDDDDKGSMNNQKVVAVLLQECKQ
VLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKW
QYKQAVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDR
FLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARISVNSGKLLKAEY
ILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAEL
IWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLS
LLKEFDHHLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDC
PPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQELHSFVKAAFGLTTVHR
RLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQVK
EHLQVQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSV
CEVFESDCGNNKNEQKDAKTGVCITALKTEIKNIDTVSTTQEKPHCQRDT
GISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQDVETETEPSDYSNGE
GAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHL
VDTQCSTALSEELENDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQ
QQMPLTPFSPHNTPGIFLAPGAGLLEGAPEGIQEVRNMGPRNTSAHSRPS
YRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKDRQGKEQG
EEISERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISML
PCSSFTPNWPVQNPDSRKSGGPVAEQGIDPDASTVDEEGQLLDSMDVPCT
NGHGSHRLCILRQPPGQRAETPNSSVSGNILFPVLSEDCTTTEEGNQPGN
MLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMR
KEILEARTLQPDDFEKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLL
KYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVG
MDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTIL
LILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSY
EFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFY
FFNNQHVECNEICHRLSLTRPSMEKPCTDYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Full Snapgene Map
pcDNA5-FRT-TO-MBP-3FLAG-ALPK1 K1067M-C-FLAG680.75 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU65884
Unit Source
Location
PPU
MTA
Notes
Parental 40934
3FLAG inserted with annealed oligos RT12030/31 into 65764
3FLAG frame with MBP then restored with NW7639/40
3FLAG inserted with annealed oligos RT12030/31 into 65764
3FLAG frame with MBP then restored with NW7639/40
Low Temp
No
Price
£125.00
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