cDNA Clone HOIP

• Plasmid: pGEX6P-1-HOIP R480-D636 V492E/I495E/Q613A/L617A/F620A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-HOIP R480-D636 V492E/I495E/Q613A/L617A/F620A
• Genbank: NM_017999.5
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCGCCAAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGaGAGCATGgaaCGGGAAGGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTACTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAACCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTAgcGCGCCAACGCgcAGAGCCCgcCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCTTCCTGGGATtgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRQDKMREEGLQLESMEREG
EAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGA
FSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQAL
FQHGGDVSRALTELARQRAEPARQRLWDSGPEPTPSWD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-HOIP R480-D636 V492E/I495E/Q613A/L617A/F620A154.22 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65914
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-term GST tag for bacterial expression of D-UBA domain of HOIP.
• Low Temp: No